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Biomedical subjects

C Chan

Publications and source records attributed to C Chan.

At least 253 records · Page 14Linked to original sources
PubMed1984

Identification and preliminary mapping with monoclonal antibodies of a herpes simplex virus 2 glycoprotein lacking a known type 1 counterpart.

The properties of herpes simplex virus 2 (HSV-2)-specific proteins reactive with monoclonal antibody H966 derived from mice immunized with HSV-2 strain G are reported. The reactive proteins contained in infected cell lysates subjected to electrophoresis in denaturing gels and transferred to nitrocellulose sheets form a relatively sharp band characteristic of Mr 124,000 proteins and a diffuse, more slowly migrating band. Antigens reactive with H966 were detected on the surface of viable, unfixed cells. The electrophoretic mobility of the H966-reactive proteins made in the presence of tunicamycin was more rapid than that of the proteins made in the absence of the drug. Direct evidence that the HSV-2-specific antigen was a glycoprotein emerged from purification of [14C]glucosamine-labeled proteins with similar electrophoretic mobilities by immunoabsorption to H966 bound to Sepharose beads. Analyses of the reactivity of HSV-1 X HSV-2 recombinants indicated the gene specifying the glycoprotein maps in the S component of the DNA. The glycoprotein detected by H966 has no known counterpart in HSV-1 and corresponds to the glycoprotein previously designated as gC of HSV-2 and reported to map to the right of gC specified by HSV-1. Inasmuch as an HSV-2 gene colinear with HSV-1 gC has been reported to specify a glycoprotein currently designated as gC of HSV-2 by Para et al. [J. Virol. 45, 1223-1227 (1983)], the glycoprotein identified by H966 should be designated as gG.

Animals↗
PubMed1984

The murine IL 2 receptor. II. Monoclonal anti-IL 2 receptor antibodies as specific inhibitors of T cell function in vitro.

We assessed the dependency of a variety of immune responses for IL 2 in vitro by using anti-IL 2 receptor monoclonal antibodies as specific inhibitors of IL 2 function. The generation of allogeneic cytotoxic T lymphocyte (CTL) responses and maximal thymocyte proliferation to phytohemagglutinin (PHA) and IL 1 was readily susceptible to inhibition by these antibodies. Furthermore, the IL 2 receptor-positive, IL 2-responsive cell in the CTL cultures expressed killer cell activity. A greater variability in susceptibility to anti-IL 2 receptor antibody inhibition was noted for proliferation of T cells to concanavalin A, PHA, or allogeneic cells. Under certain conditions, however, each of these responses was almost completely inhibited. In most instances, the failure to block a response could be accounted for by either high levels of endogenous IL 2 production or high density of cell surface IL 2 receptors, which represent two known variables that influence the level of inhibition by these antibodies. Analysis of IL 2 receptor expression by mitogen-stimulated T cells suggested that accessory cells may play a role in the optimal expression of the IL 2 receptor. These experiments demonstrate that IL 2 is the predominant growth factor by which T lymphocytes proliferate, but do not exclude the possibility of an IL 2-independent pathway for growth.

Animals↗
PubMed1983

Continuous five-day infusion of PALA and 5FU: a pilot phase II trial.

In order to determine whether a simultaneous infusion of N-(phospho-n-acetyl)-L-aspartic acid (PALA) was able to increase the rate of antitumor response to 5-fluorouracil (5-FU), a pilot study was conducted. Of 10 evaluable patients with previously drug-untreated colorectal carcinoma, there were only two partial responses, lasting 2.3 and 1.6 months. No partial responses were observed in three evaluable patients with soft tissue sarcomas. The dose-limiting toxicity was dermatitis. The simultaneous infusion of PALA and 5-FU is not likely to produce a high number of antitumor responses of long duration in patients with colorectal carcinoma.

Adult↗
PubMed1983

Fine specificity of cloned insulin-specific T cell hybridomas: evidence supporting a role for tertiary conformation.

Two groups of cloned insulin-specific T cell hybridomas were derived from the fusion of (BALB/c X A/J)F1 T cells with the BW5147 tumor line. Group I responded only to the immunogen pork insulin and failed to respond to rat insulin, which differs only at amino acids A4 and B3. The second group of T cell hybridomas exhibited a broader pattern of reactivity to heterologous insulins because they could be stimulated with the B chain-identical pork, beef, sheep, and human insulins, as well as by isolated oxidized B chain. These hybridomas, however, displayed a much greater reactivity to pork insulin than to beef insulin or to the oxidized B chain. Comparison of the reactivity profiles of these two groups of hybridomas with the three-dimensional structure of the insulin molecule strongly suggested that group I cells were recognizing an immunogenic moiety composed of residues A4 and/or B3 in association with A chain loop determinants and that group II hybrids recognized a moiety composed of the amino acid sequence of the B chain and the A chain loop. Both groups of hybrids were restricted to the high-responder I-Ad allele but could be distinguished by their pattern of alloreactivity. We were unable to show any effect of a panel of monoclonal anti-insulin antibodies on the induction of interleukin 2 secretion by these hybridomas or to demonstrate the presence of idiotypic determinants on these T hybrids by using a panel of anti-idiotypic reagents raised against the monoclonal anti-insulin antibodies.

Animals↗
PubMed1983

Herpes zoster ophthalmicus with contralateral hemiparesis. A case report and review of the literature.

An 80-year-old man developed right herpes zoster ophthalmicus complicated by contralateral hemiparesis after right cataract extraction. Although herpes zoster ophthalmicus is a frequent occurrence among the elderly, the association with contralateral hemiparesis is probably overlooked by many ophthalmologists. A review of the literature has been made to outline the etiology of this problem and to make diagnosis more likely. The varicella virus probably spreads in a retrograde fashion from the gasserian ganglion toward the cavernous sinus to involve cranial nerves 3, 4, or 6. Involvement of the ipsilateral carotid arterial system by the progressive inflammatory reaction can result in the contralateral hemiparesis. Standard therapy includes topical steroids and topical antibiotics for the ocular and surface lesions. The efficacy of systemic steroids to minimize the hemiparesis is still in question.

Aged↗
PubMed1983

Resistance to hepatotoxins acquired by hepatocytes during liver regeneration.

The appearance of resistance to a number of hepatotoxins in primary cultures of hepatocytes prepared at various time intervals up to 2 weeks after partial hepatectomy is the major focus in this study. Resistance to the cytocidal effect of aflatoxin B1, 2-acetylaminofluorene, N-hydroxy-2-acetylaminofluorene, methotrexate, or methyl methanesulfonate shows a progressive increase until 48 hr and then returns to the resting level of susceptibility by 2 weeks. The genesis of mutagens from 2-acetylaminofluorene and aflatoxin B1 by S-9 liver fractions shows a decrease from and return to control values after partial hepatectomy that parallels the resistance. The levels of total cellular cytochromes P-450 also decrease following partial hepatectomy and remain from 28 to 36% less than those of controls for at least 1 week. The glutathione and total soluble sulfhydryl ("glutathione") content increase following partial hepatectomy, and the pattern is consistent with a partial role for glutathione in the resistance phenomenon as it relates to 2-acetylaminofluorene. The possible relationship between resistance to the cytocidal effects in vitro and the resistance to inhibition of cell proliferation in vivo during liver carcinogenesis is discussed.

2-Acetylaminofluorene↗
PubMed1982

Nature of the antigenic complex recognized by T lymphocytes. VIII. Specific inhibition of the stimulatory capacity of antigen-pulsed hapten-modified peritoneal exudate cells by anti-hapten antibody.

When macrophages which were pulsed with soluble protein antigens and then modified with 2,4,6-trinitrobenzenesulfonic acid (TNBS) were cultured with primed T cells in the presence of anti-2,4,6-trinitrophenyl (TNP) antiserum, a marked inhibition of the T cell proliferative response specific for the soluble protein antigen under study was observed. Inhibition of the proliferative response required that the protein antigen under study be easily susceptible to conjugation with TNBS. A lag period of 1-3 h must elapse following pulse exposure to the protein antigen and prior to TNP modification for anti-TNP antibody to exert an inhibitory effect, which suggests that processing of antigen in an intracellular site might occur with subsequent reexpression of antigen on the macrophage surface. It was hypothesized that anti-TNP antibody inhibits T cell proliferation by capping and shedding of TNP-modified determinants of the nominal antigen as well as TNP-modified cell surface proteins from the macrophage membrane. The failure of others to demonstrate an effect of anti-antigen antibody on T cell activation can best be explained as secondary to the low density of antigenic determinants on the macrophage membrane with resultant low-affinity binding of antibody to antigen.

Animals↗
PubMed1982

Isocratic high-performance liquid chromatographic method for the determination of tricyclic antidepressants and metabolites in plasma.

An isocratic high-performance liquid chromatographic method for the determination of six tricyclic antidepressants and their major metabolites is presented. Hexane containing 0.5% diethylamine was used as an extraction solvent to minimize adsorption onto glass. A reversed-phase cyanopropylsilane column was used with a mobile phase consisting of 70% acetonitrile and 30% 0.03 M acetate buffer, pH 7.0. Good identification and quantitation were obtained by the use of both UV detection at 245 nm and spectrofluorometric detection with an excitation wavelength of 276 nm and an emission filter with a 370-nm cutoff. A minimum detectable limit of less than 5 ng/ml of plasma is possible with this system. The reproducibility and precision of the method are shown from the analysis of samples containing 20-400 ng/ml in plasma.

Adsorption↗
PubMed1982

Inexpensive fiber optic two-dimensional densitometer for the high-resolution quantitation of autoradiogram grain densities.

The design and construction of a simple, low cost high performance fiber optic 2-dimensional microdensitometer is described. With this instrument the film to be quantitated is placed upon a back-lit frosted glass bench and scanned with an optic fiber probe attached to a transparent micromanipulator. The emerging light is transported by the fiber to the photocell of a direct reading spectrophotometer. The resulting numerical data can either be transcribed, portrayed on a recorder or entered into the memory of a microdata processor for further analysis and comparisons. The instrument was sensitive to very small differences in optical density and could resolve lines 100 but not 30 micrometers apart. It was precise, reliable and easy to build. With the use of this equipment many previously undetected, significant local brain glucose utilization differences were quantitated in groups of unrestrained rodents, maintained in several behavioral states.

Animals↗
PubMed1982

Induction of transplantation tolerance in guinea pigs by spleen allografts. II. Responses in the mixed leukocyte reaction correlate with the tolerant state.

We have developed a model for the induction of transplantation tolerance in the guinea pig by vascularized spleen allografts. Spleen allografts from strain 13 to strain 2 hosts frequently survived in healthy recipients without clinical GVHD or induced clinical GVHD. (2 x 13)F1 to strain 2 spleen allografts survived indefinitely without inducing GVHD. In contrast, strain 2 spleen allografts were rejected by strain 13 hosts. An excellent correlation was observed between the clinical course and the degree of reactivity to donor strain stimulator cells in the MLR. Animals that had rejected their grafts had normal or enhanced proliferative responses in the MLR. Strain 2 hosts with long-term surviving strain 13 or (2 x 13)F1 grafts had markedly suppressed anti-13 responses. Animals with GVHD had a suppressed MLR toward donor strain stimulator cells with simultaneous reactivity to host strain stimulator cells. Cells capable of suppressing the response of normal host strain cells to donor strain stimulators were present in some long-term surviving animals and may be responsible in part for the maintenance of the tolerant state.

Animals↗
PubMed1981

Medical staff and hospital administration uses of discharge abstract data.

In summary, this article suggests several useful approaches whereby physicians and hospital administrators can efficiently sort through large amounts of discharge abstract data to identify problem areas, particularly those concerning quality of care and utilization of services, and to formulate strategies for improvement. Approaches and steps suggested include: . Review objectives-determining variables of greatest concern; . Standards-determining acceptable values or levels of performance; . Focus of review-determining the unit of review, such as specific departments, diseases, or physicians; and . Quality and reliability of discharge abstract data-determining the data to use by undertaking four levels of edits: technical edits, logical edits, ratio edits, and categorical edits. A model flowchart provided in this article serves as a conceptual framework by which potential problem areas can be studied during a patient's hospitalization to precisely identify the interaction, the time, and the cause/association of the problem.

Decision Making↗
PubMed1980

Important issues in the drug treatment of schizophrenia.

The large body of research demonstrating the effectiveness of antipsychotic drugs in the treatment of acute schizophrenia is selectively reviewed. Research evidence relevant to the following issues is assessed; indications for selective treatment; characteristics of drug responders and nonresponders; indications for high dosage phenothiazine treatment; indications for maintenance therapy; benefits and risks of antipsychotic drugs. Recommendations are made concerning areas of psychopharmacologic research that require further development.

Antipsychotic Agents↗