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Biomedical subjects

C Cavadini

Publications and source records attributed to C Cavadini.

25 records · Page 2Linked to original sources
PubMed1994

Malondialdehyde measurement in urine.

Malondialdehyde (MDA) is an end product of lipid peroxidation and is a frequently measured index of these processes. The thiobarbituric acid (TBA) test is commonly used to measured MDA, but its specificity is questionable due to the presence of interfering chromogens. Wade and van Rij described in 1988 a method which removes these chromogens by HPLC. However, the sensitivity and the resolution of this method was not adequate for measurements of MDA in urine. We have improved this method by replacing TBA with diethylthiobarbituric acid (DETBA). The less polar MDA-DETBA complexes were isolated on Bakerbond cartridges and quantified by HPLC without interference. MDA was detectable using a fluorescence or ultraviolet detector at picomole levels. This technique was applied to urine samples obtained from ten burns patients on different days following their hospitalization. Urinary MDA in burns patients was very high and reached 18.6 mumol/mmol creatinine in one patient compared with a mean value of 0.23 mumol/mmol creatinine in healthy controls. Maximum MDA levels were attained on the third day for the majority of patients and remained, on average, much higher than normal even after 20 days. Using this method, picomole quantities of MDA can be easily and specifically detected in urine samples. This method is useful for assessing an oxidative stress.

Burns↗
PubMed1994

[Unrecognised intake of trace elements in polytraumatized and burnt patients].

Recommended trace element doses during parenteral nutrition have been revised many times and increased, especially in surgical patients. Blood products are known to provide significant amounts of trace elements under certain circumstances. In intensive care patients crystalloids and colloids are also given in large amounts. This study aimed at determining the magnitude of the unrecognized trace element administrations after major burns and/or trauma. Fifteen patients burnt 36 +/- 11% (mean +/- SD) of body surface area, aged 34 +/- 8 years, admitted to the Burns Centre and 11 trauma patients with an Injury Severity Score of 29 +/- 6 points, aged 40 +/- 13 years, admitted to the surgical Intensive Care Unit in a Swiss University Hospital. Prospective study of intakes and urinary excretion from the first post-injury day (D1) to D7. Copper and zinc were analyzed by flame atomic absorption spectrophotometry, and selenium by fluorimetry. The actual trace element administrations were much larger than those prescribed in the 3 groups of patients, and were significantly above the most recent parenteral recommended daily allowance = RDA (2.3 times RDA for copper, 5 times for selenium and zinc in Group 2, which received the largest i.v. supplements). There greatest provision of the 3 elements was by the blood products (packed red cells and frozen plasma) and by the albumin solutions (0.5 mg.L-1 Cu, 90 micrograms.L-1 Se and 2.1 mg.L-1 Zn in the 20% solutions). During the resuscitation phase, crystalloids provided a significant amount of copper (0.14 mg.L-1 NaCl 0.9%, none in dextrose) and zinc (0.3 mg.L-1 of any crystalloid), whereas selenium was not detected.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1992

Selenium losses in 10 burned patients.

To determine the selenium (Se) losses and balances, 10 patients with burns of 32 +/- 9% (mean +/- SD) of total body surface and aged 36 +/- 9 years were studied from D1 (first post-injury) unitl D7. Cutaneous losses were extracted from the textiles surrounding the patients. Serum and urine were collected until D20. Exudative losses of nitrogen decreased progressively (mean 8.7 +/- 3.8 g/24H). Se was detectable in wound seepage only during excision-grafting: mean operative loss was 342 +/- 191 mug. Mean urinary Se excretion was 41 +/- 13 mug/24H. Operative cutaneous losses led to some negative balances. Serum Se and glutathione peroxydase (GSHPx) were depressed until D20. Serum Se was related to N intake (p < 0.001). The decreased Se and GSHPx levels reflect a deficiency state, which measured losses did not explain, but limitations of the measurement methods prevented detection of Se cutaneous losses 100 mug/24H.

Journal Article↗
PubMed1992

Cutaneous copper and zinc losses in burns.

To measure the exudative cutaneous copper (Cu) and zinc (Zn) losses in burns, 10 patients, aged 36 +/- 9 years (mean +/- s.d.) with burns covering 33 +/- 10 per cent of the total body surface area, were studied from the first postburn day (D1) until D7. All intakes and losses were analysed for Cu, Zn and nitrogen (N) content. Cutaneous losses were extracted from textiles surrounding the patients. Urinary excretions were 0.12 +/- 0.06mg/24h for Cu, 0.9 +/- 0.6mg/24h for Zn, and 14.1 +/- 4.4g/24h for N. Mean daily exudative losses through wound seepage from D1 to D7 were 4.7 +/- 2.1mg/24h for Cu, 27.1 +/- 14.4mg/24h for Zn, and 8.7 +/- 3.8g/24h for N. The cumulated mean losses over 7 days were 37mg for Cu, and 212mg for Zn, representing respectively 20-40 per cent and 5-10 per cent of normal body content. Serum Cu and Zn levels were strongly depressed. The urinary Cu/N ratios correlated with clinical improvement. We conclude that the exudative Cu and Zn losses during the first week postburn contribute significantly to the increased nutrient requirements in burns.

Adult↗
PubMedDate not supplied

Influence of large intakes of trace elements on recovery after major burns.

Because Cu, Se, and Zn are involved in immune and antioxidative defense mechanisms and tissue repair, deficiencies might aggravate complications classically observed with burns. After measuring massive cutaneous trace element losses in 10 burn patients, our aim in this study was to determine whether large intravenous intakes of Cu, Zn, and Se can modify serum trace element levels and recovery after major burns. Ten patients, aged 34 +/- 6 yr (mean +/- SD), admitted to the burns center of a Swiss university hospital with thermal burns on 41 +/- 9% of their body surface were studied prospectively, with trace element balance studies from day 1 (D1) to D7 postinjury. Urine and blood samples were also collected on D10, D15, D20, and D25. The patients were divided into two groups of five and received either standard (group 1, control) or greatly increased (group 2, treatment: 4.5 mg Cu, 190 micrograms Se, and 40 mg Zn/day) trace element intakes. Energy and protein intake and wound treatment were similar in both groups. The treatment group was characterized by improved Cu, Se, and Zn status (increase in serum levels and various protein indicators), a much larger leukocyte increase between D4 and D14 (mainly neutrophils), and shorter hospital stay (45 days) compared with the untreated group (57 days). Grafting requirements were more extensive in group 1. Although severity of injury and wound treatment were similar in the groups, the duration of hospitalization was lower in the treated group. Further studies are required to determine whether this is related to trace element supplementation.

Adult↗