Search PubMed⌕ Search

Biomedical subjects

C Casanova

Publications and source records attributed to C Casanova.

At least 37 records · Page 2Linked to original sources
PubMed2000

Responses of neurons in the cat posteromedial lateral suprasylvian cortex to moving texture patterns.

The posteromedial lateral suprasylvian cortex represents a point of convergence between the geniculostriate and extrageniculostriate visual pathways. Given its purported role in motion analysis and the conflicting reports regarding the texture sensitivity of this area, we have investigated the response properties of cells in PMLS to moving texture patterns ("visual noise"). In contrast to previous reports, we have found that a large majority of cells (80.1%) responds to the motion of a texture pattern with sustained discharges. In general, responses to noise were more broadly tuned for direction compared to gratings; however, direction selectivity appeared more pronounced in response to noise. The majority of cells was selective for drift velocity of the noise pattern (mean optimal velocity: 26.7 degrees /s). Velocity tuning was comparable to that of its principal thalamic input, the lateral posterior pulvinar nucleus. In general, responsiveness of cells in the posteromedial lateral suprasylvian cortex increased with increasing texture element size, although some units were tuned to smaller element sizes than the largest presented. Finally, the magnitude of these noise responses was dependent on the area of the visual field stimulated. In general, a stimulus corresponding to roughly twice the size of the receptive field was required to elicit an equivalent half-maximal response to that for gratings. The results of this study indicate that the majority of cells in the posteromedial lateral suprasylvian cortex can be driven by the motion of a fine texture field, and highlight the importance of this area in motion analysis.

Animals↗
PubMed2000

Long-term controlled trial of nocturnal nasal positive pressure ventilation in patients with severe COPD.

STUDY OBJECTIVES: To determine the 1-year efficacy of noninvasive positive pressure ventilation (NPPV) added to long-term oxygen therapy (LTOT) in patients with stable severe COPD. PATIENT SELECTION AND METHODS: We prospectively randomized 52 patients with severe COPD (FEV(1) < 45%) to either NPPV plus "standard care" (96% patients with LTOT) or to standard care alone (93% patients with LTOT). The outcomes measured included the following: rate of acute COPD exacerbations; hospital admissions; intubations; and mortality at 3 months, 6 months, and 12 months. The patients were also evaluated at 3 months and 6 months for dyspnea using the Medical Research Council and Borg scales, gas exchange, hematocrit, pulmonary function, cardiac function with echocardiogram, and neuropsychological performance. RESULTS: One-year survival was similar in both groups (78%). The number of acute exacerbations was similar at all time points in patients receiving NPPV, compared with control subjects. The number of hospital admissions was decreased at 3 months in the NPPV group (5% vs 15% of patients, p < 0.05), but this difference was not seen at 6 months (18% vs 19%, respectively). The only beneficial differences were observed in the Borg dyspnea rating, which dropped from 6 to 5 (p < 0.039), and in one of the neuropsychological tests (psychomotor coordination) for the NPPV group at 6 months. CONCLUSIONS: Our study indicates that over 1 year, NPPV does not affect the natural course of the disease and is of marginal benefit in outpatients with severe COPD who are in stable condition.

Acute Disease↗
PubMed1999

Chagas disease in an area of recent occupation in Cochabamba, Bolivia.

INTRODUCTION: A descriptive, entomological and seroepidemiological study on Chagas disease was conducted in a place of recent occupation on the outskirts of Cochabamba, Bolivia: Avaroa/Primer de Mayo (population:3,000), where the socio-economic level is low and no control measures have been made available. METHODS: The immunofluorescent antibody test (IFAT) was used for IgG and IgM anti-Trypanosoma cruzi antibodies in filter paper bloodspot eluates from 128 subjects (73 females, 55 males) selected by systematic sampling. Concerning each subject age, gender, birthplace, occupation, duration of residence and building materials used in their houses were recorded. Vectors were captured both in domestic and peridomestic environments. RESULTS: Seropositive, 12.5% (16/128): females, 15.1% (11/73); males, 9.1% (5/55). Average time of residence: 6.1 years for the whole population sample and 7.4 years for the seropositive subjects. Most houses had adobe walls (76. 7%, n= 30), galvanized iron rooves (86.7%) and earthen floors (53. 4%) 80% of the walls had crevices. One hundred forty seven specimens of Triatoma infestans were captured, of which 104 (70.7%) were domestic, and 1 peridomestic Triatoma sordida. Precipitin host identification: birds, 67.5%; humans, 27.8%; rodents, 11.9%; dogs, 8. 7%; cats, 1.6%. House infestation and density indices were 53.3 and 493.0 respectively. We found 21 (14.3%) specimens of T. infestans infected with trypanosomes, 18 (85.7%) of which in domestic environments. DISCUSSION: The elements for the vector transmission of Chagas disease are present in Avaroa/Primer de Mayo and the ancient custom of keeping guinea pigs indoors adds to the risk of human infection. In neighboring Cochabamba, due to substandard quality control, contaminated blood transfusions are not infrequent, which further aggravates the spread of Chagas disease. Prompt action to check the transmission of this infection, involving additionally the congenital and transfusional modes of acquisition, is required.

Adolescent↗
PubMed1998

Motion integration in a thalamic visual nucleus.

Thalamic nuclei have long been regarded as passive relay stations for sensory information en route to higher level processing in the cerebral cortex. Recently, physiological and theoretical studies have reassessed the role of the thalamus and it has been proposed that thalamic nuclei may actively participate with cortical areas in processing specific information. In support of this idea, we now show that a subset of neurons in an extrageniculate visual nucleus, the lateral-posterior pulvinar complex, can signal the true direction of motion of a plaid pattern, indicating that thalamic cells can integrate different motion signals into a coherent moving percept. This is the first time that these computations have been found to occur outside the higher-order cortical areas. Our findings implicate extrageniculate cortico-thalamo-cortical loops in the dynamic processing of image motion, and, more generally, as basic computational modules involved in analysing specific features of complex visual scenes.

Animals↗
PubMed1998

Persistent pulmonary hypertension of the neonate and asymmetric growth restriction.

OBJECTIVE: To evaluate the possible associations between persistent pulmonary hypertension of the neonate, need for extra-corporeal membranous oxygenation, small for gestational age (SGA), and low ponderal index for gestational age in infants with persistent pulmonary hypertension of the neonate and in matched controls. METHODS: Eighty-six infants with persistent pulmonary hypertension of the neonate delivered from 1991 to 1994 at our hospital were matched with 430 contemporaneous control singleton neonates. Birth weight and ponderal indices (100 x weight/length3) less than the tenth percentile for gestational age and gender were defined as SGA and low ponderal index, respectively. We assessed associations between these markers, the presence of persistent pulmonary hypertension of the neonate, and the need for extracorporeal membranous oxygenation. RESULTS: Low ponderal index was associated with persistent pulmonary hypertension of the neonate (odds ratio [OR] 5.4), whereas SGA was not. Low ponderal index (OR 4.0) was an independent correlate of persistent pulmonary hypertension of the neonate after adjustment with logistic regression for 5-minute Apgar scores less than 7, umbilical arterial pH less than 7.10, and presence of meconium. Low ponderal index was associated with need for extracorporeal membranous oxygenation in neonates with persistent pulmonary hypertension (P < .001). CONCLUSION: Fetal developmental events may significantly affect neonatal pulmonary status. Diminished neonatal nutritional status, as measured by low ponderal index for gestational age, is associated with increased risk of persistent pulmonary hypertension of the neonate and severity of the disease process.

Case-Control Studies↗
PubMed1998

Spatial frequency processing in posteromedial lateral suprasylvian cortex does not depend on the projections from the striate-recipient zone of the cat's lateral posterior-pulvinar complex.

It is generally considered that the posteromedial part of the cat's lateral suprasylvian cortex is involved in the analysis of image motion. The main afferents of the posteromedial lateral suprasylvian cortex come from a direct retinogeniculate pathway and indirect retinotectal and retino-geniculo-cortical pathways. Removal of the primary visual cortex does not affect the spatial and temporal processing of suprasylvian cortex cells suggesting that these properties are derived from thalamic input. We have investigated the possibility that the striate-recipient zone of the lateral posterior nucleus-pulvinar complex may be responsible for the spatial (and temporal) frequency processing in posteromedial lateral suprasylvian cortex since these two regions establish strong bidirectional connections and share many visual properties. Experiments were done on anaesthetized normal adult cats. Visual responses in suprasylvian cortex were recorded before, during, and after the deactivation of the lateral part of the lateral posterior nucleus accomplished by the injection of lidocaine or GABA. Results can be summarized as follows. A total of 64 cells was tested. Out of this number, 11 units were affected by the deactivation of the lateral part of lateral posterior nucleus and one cell, by the blockade of pulvinar. For all cells, except one, the effect consisted in a global reduction of the evoked discharge rate suggesting that the thalamo-suprasylvian cortex projections are excitatory in nature. We did not find any significant differences in the optimal spatial frequency, nor in the width of the tuning function, whether the grating was presented at half- or saturation contrast. In addition, there were no significant differences between the low- and high cut-off spatial frequency values computed before and after the deactivation of the lateral posterior nucleus. No specific changes were observed in the contrast sensitivity function of the posteromedial lateral suprasylvian cortex cells. Similar results were observed with respect to the temporal frequency tuning functions. Deactivating the lateral posterior nucleus did not modify the direction selectivity nor the organization of the subregions of the lateral suprasylvian cortex "classical" receptive fields. The absence of strong changes in posteromedial lateral suprasylvian cortex cell response properties following the functional blockade of the lateral posterior nucleus suggests that the projections from this part of the thalamus are not essential to generate the spatial characteristics of most posteromedial lateral suprasylvian cortex receptive fields. These properties may be derived from other thalamic inputs (e.g., medial interlaminar nucleus) and/or from the intrinsic computation of the afferent signals within the lateral suprasylvian cortex. On the other hand, it is possible that the lateral posterior nucleus lateral suprasylvian cortex loop may be involved in other functions such as the analysis of complex motion as suggested by the findings from our and other groups.

Animals↗
PubMed1998

The impact of patient experience on practice: the acceptability of emergency contraceptive pills in inner-city clinics.

OBJECTIVES: This article reports on a study of 119 women who sought and used emergency contraceptive pills (ECPs) at Planned Parenthood of New York City (PPNYC) clinics between June 1996 and May 1997. It focuses on their satisfaction with the method, their attitudes toward ECPs, their reactions to the service, and the impact their perceptions had on changing the provision of care. METHOD: The PPNYC clinical protocol employed the Yuzpe method and fairly conservative procedures, including restrictive screening, a pelvic examination for all new patients, and limited appointment slots. A two-part survey captured information on patient experience with ECPs. RESULTS: The largest group of respondents (40%) found out about ECPs from friends. Almost 90% of the women were using contraception before their visit to the clinic. Sixty-eight percent reported that they sought ECPs because the condom failed. In the follow-up, a majority (57%) reported that they intended to change or had changed their method of contraception--more than three-quarters to a hormonal contraceptive. While generally satisfied with the service, many respondents were cautious of more extensive distribution of ECPs. CONCLUSION: The survey results had a profound impact on services: PPNYC revised the ECP protocol, developed a staff training package, expanded its service, and planned a multidimensional public media campaign. Further research, including a closer examination of participants' cautious attitude toward unrestricted distribution of ECPs, will be needed as PPNYC expands access to ECP.

Adolescent↗
PubMed1997

Dose-dependent inhibitory effects of angiotensin II on visual responses of the rat superior colliculus: AT1 and AT2 receptor contributions.

Angiotensin II (Ang II) has traditionally been regarded as a peripherally circulating and acting hormone involved in fluid homeostasis and blood pressure regulation. With the rather recent localization of Ang II receptors within the mammalian brain, renewed interest has emerged in the hope of elucidating the central impact and function of this hormone. One region that has been clearly demonstrated to express Ang II receptors is the superior colliculus (SC). This mesencephalic structure plays an important role in sensory visuomotor integration. Receptors for Ang II (of both the AT1 and AT2 subtypes) have been localized within the superficial layers of this structure, i.e. the areas that are visually responsive. In the hopes of characterizing the role of Ang II in the SC, we have attempted to physiologically activate these receptors in vivo and observe the effects of Ang II on visually evoked responses. In the attempt to identify the receptor subtype(s) responsible in mediating these effects, Ang II was injected concomitantly with selective receptor ligands. Experiments were performed on adult rats prepared in classical fashion for electrophysiological studies. Through microinjection of Ang II, and the simultaneous recording of visually evoked potentials to flash stimulation, we have observed that this peptide yields a strong suppressive effect on visual neuronal activity. By injecting Ang II at various concentrations (10(-3)-10(-10) M), we have further observed that the effects of this peptide express a dose-related dependency. Injection of Ang II in progressively more ventral layers yielded less pronounced effects, demonstrating physiologically the discrete localization of these receptors in the stratum griseum superficiale. Coinjection of Ang II with Losartan yielded a near complete blockade of Ang II suppressive effects, suggesting that AT1 receptors play a prominent role in mediating these responses. However, coinjection of Ang II with PD 123,319 yielded a slight, yet significant partial blockade. Coinjection of Ang II with both the AT1 and AT2 receptor antagonists yielded a complete blockade of the Ang II effect. Finally, some of the results suggest that the AT2 receptor ligand CGP 42,112 may possess agonist properties. Taken together, these findings suggest that the AT1 receptor is predominantly involved in mediating Ang II responses in the SC and there also appears to be some indication of AT2 receptor involvement. However, the underlying mechanisms (such as receptor interactions), the exact specificity of the ligands used, and the possibility of other receptor subtype implication have yet to be explored fully.

Angiotensin II↗
PubMed1997

Contribution of area 17 to cell responses in the striate-recipient zone of the cat's lateral posterior-pulvinar complex.

The cat's lateral posterior-pulvinar complex (LP-pulvinar) contains three main representations of the visual field. The lateral part of the LP nucleus (LPl or striate-recipient zone) is the only region of these extrageniculate nuclei which receives afferents from the primary visual cortex. We investigated the contribution of area 17 to the response properties (orientation and spatial frequency tuning functions) of LPl neurons by cooling or lesioning the visual cortex. Responses of 40 LPl cells were studied before, during and after the reversible cooling of the striate cortex. When tested for orientation, a total of 10 units out of 28 was affected (36%). For most of these cells (eight of 10), cooling the visual cortex yielded a reduction of the cells' visual responses without altering their orientation-selectivity (there was no significant change in the orientation tuning width). For only two cells, inactivation led to an increase in the response amplitude. Also, blocking the visual cortex never modified the direction-selectivity of LPl cells. When tested for spatial frequency, 12 neurons out of 33 were affected (36%) by the experimental protocol. In most cases, we observed a reduction in the responses at each spatial frequency tested, with no change in tuning bandwidth. For only three LPl cells, the effects of inactivation of the visual cortex were restricted to specific spatial frequencies, altering the profile of the spatial frequency tuning function. In five cats, removing area 17 reduced the proportion of visual neurons in LPl and the spared visually evoked responses were noticeably depressed. Despite the reduction in responsiveness, a few LPl receptive fields within the cortical scotoma were still sensitive to the orientation and/or direction of a moving stimulus. This last observation suggests that some properties in LPl could be generated either by circuits intrinsic to the LPl or by afferents from extrastriate cortical areas. Overall, these results indicate that projections from the visual cortex to the striate-recipient zone of the LP-pulvinar complex are mainly excitatory. Despite the strong impact of the area 17 projections, our data suggest that the extrastriate cortex could also play a role in the establishment of response properties in the cat's LPl.

Animals↗
PubMed1997

Apomorphine modifies the visual responses of cells in the rabbit's lateral geniculate nucleus.

It has been shown that enhancing or reducing dopaminergic activity in the retina modifies the balance between center and surround responses of retinal neurons such as ganglion cells. We investigated how these changes are reflected in the dorsal lateral geniculate nucleus (dLGN) by studying the effects of injections of apomorphine, a mixed D1 and D2 agonist of dopamine, on the visual responses of geniculate cells. Experiments were carried out on anesthetized adult pigmented rabbits. A varnished tungsten microelectrode was used to record single-unit activity in the dLGN. The flash electroretinogram was also recorded to monitor retinal changes and to confirm the success of the injections. Apomorphine was injected intravitreally or intravenously. The results can be summarized as follows. Apomorphine decreased the amplitude of the b-wave of the electroretinogram. For most dLGN cells, apomorphine produced a strong reduction in response amplitude evoked by sine-wave grating stimuli, presented at various spatial frequencies. Responses to flashing spots were also reduced but to a much lesser extent than those to gratings. In addition, the balance between the responses to small and large spots changed in favor of large stimuli. Consequently, after injection of apomorphine, the geniculate cells were preferentially activated by large-sized flashing stimuli. These data suggest that apomorphine can reduce the spatial contrast sensitivity of cells in the dLGN. This effect could be mediated by the reduction of the strength of lateral inhibition at the retinal level.

Animals↗
PubMed1997

[Type 1 Gaucher disease in a pediatric patient: 12 year's evolution].

A 14 years old patient, diagnosed in early childhood of type 1 Gaucher's disease is reported to show his unusual genotype and clinical course. The patient is heterozygous for mutation D409H, that in the homozygous patient determines an aggressive disease accompanied by neurological signs, and for a new mutation, which must be considered of mild effects. Early detection of bone involvement is emphasized as a factor to consider when indicating of enzyme replacement therapy.

Alleles↗
PubMed1996

Responses to moving texture patterns of cells in the striate-recipient zone of the cat's lateral posterior-pulvinar complex.

We have studied the response properties of cells in the lateral part of the lateral posterior nucleus or striate-recipient zone (LPl) of the lateral posterior nucleus-pulvinar complex to the motion of textured patterns [visual noise]. Our purpose was to determine basic noise response characteristics and to compare these properties to that of cells in area 17 known to project to the LPl. Practically all LPl cells (87%) responded to the motion of visual noise. The evoked discharges were either sustained or characterized by several bursts. On average, as found in cortex, LPl neurons were more broadly tuned for the direction of noise than that of gratings (bandwidths of 49 and 36 degrees, respectively; t-test, P < 0.005). Noise tuning function in LPl was comparable to that found in cortex (mean of 48 degrees). One third of the LPl units did not exhibit any preferences for drift direction of noise. Such cells were virtually not encountered in the striate cortex. This group of LPl cells was generally not tuned for grating direction. For practically all LPl cells, responses to noise varied as a function of drift velocity. The mean optimal velocity was 27.5 degrees/s with mean bandwidth of 2.5 octaves. LPl cells were sensitive to a broader range of velocities than complex cells in area 17. The results of the present study showed that visual noise is an appropriate stimulus for studying motion sensitivity of cells in the LPl. It also revealed that the noise response properties, such as direction and velocity tuning functions, are very similar to those reported in the striate cortex. The exact contribution of area 17 in the visual noise responsiveness of LPl cells remains to be determined. This study provides additional evidence that the lateral posterior nucleus-pulvinar complex may be involved in many aspects of visual processing.

Animals↗
PubMed1996

Motion sensitivity and stimulus interactions in the striate-recipient zone of the cat's lateral posterior-pulvinar complex.

The cat's lateral posterior-pulvinar complex (LP-pulvinar) establishes reciprocal connections with the anterior ectosylvian visual (AEV) and lateral suprasylvian (LS) cortices; two regions which are believed to be involved in motion analysis. We have investigated the motion sensitivity of neurons in the LP-pulvinar complex by: (1) studying the responses properties of cells in the striate-recipient zone of the LP nucleus (LPI) to the drift of a two-dimensional texture pattern (visual noise); and (2) determining the extent to which the latter stimulus can modify the spatial frequency tuning function of LPI cells. Experiments were carried out on anesthetized normal adult cats. Almost all LPI cells (55 out of 63, 87%) responded to the motion of visual noise. For most units (39 out of 55, 71%), responses varied as a function of the direction of motion (bandwidth of 49 degrees). One-third of the LPI units did not exhibit any preference for drift direction of noise. For practically all LPI cells, responses to noise varied as a function of drift velocity. Optimal velocities were distributed from 2 to 35 degrees/s with a mean value of 27.5 degrees/s (means bandwidth of 2.5 octaves). The influence of visual noise on the spatial frequency tuning function of 22 LPI cells was also studied. For half of LPI cells, responses at all spatial frequencies were reduced when the grating and the texture pattern were moving in opposite directions (anti phase condition). This masking effect of noise was rarely observed when both stimuli were drifted in the same direction (in phase condition). These results suggest that the LP-pulvinar complex may be part of extrageniculate pathways involved in the analysis of motion of visual targets and/or the analysis of the relative movement between an object and its surrounding environment.

Animals↗
PubMed1996

Modulation of membrane potential and ionic currents by the AT1 and AT2 receptors of angiotensin II.

Angiotensin II, the principal effector of the renin-angiotensin system, modulates various ionic currents. Its effects on potassium currents, including outward transient potassium current, the inward or outward rectifiers, as well as Ca(2+)- activated potassium currents, is well described. Other ionic currents, such as voltage-dependent calcium currents, cationic or chloride currents, are also altered by the hormone. All these effects provoke changes in membrane potential, such as modulation of action potential firing or resting membrane potential and control intracellular calcium concentration. Summarized here are the results obtained on these membrane electrical properties using electrophysiological recordings.

Action Potentials↗
PubMed1996

Pediatric hospitalization due to ambulatory care-sensitive conditions in Valencia (Spain).

BACKGROUND: Studies in the United States have demonstrated that rates of hospitalization for conditions sensitive to primary care are related to socioeconomic factors. Our objective was to identify those sociodemographic and primary care factors associated with pediatric hospitalization for ambulatory care-sensitive conditions, in a country (Spain) with a health system that provides universal coverage. METHODS: Cross-sectional survey of 504 children hospitalized in a District General Hospital in Valencia, Spain. Data were gathered on sociodemographic variables, type of physician providing primary care and ambulatory care use prior to hospitalization. Analysis consisted of bivariate statistical tests and logistic regression techniques. RESULTS: Children who were under 2 years old and female were at significantly higher risk for hospitalization due to ambulatory care-sensitive conditions. Socioeconomic variables, type of physician or a previous visit to primary care services were not associated with a different risk of hospitalization due to these conditions. CONCLUSION: Characteristics unrelated to difficulties in access, or to type of provider, influence the risk of hospital admissions for conditions that could be prevented or managed without hospitalization. More specific classification of conditions potentially could be useful for determining which factors of structure or process of health services are related to hospitalization.

Adolescent↗