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Biomedical subjects

C C Smith

Publications and source records attributed to C C Smith.

At least 235 records · Page 13Linked to original sources
PubMed1976

Characterization of extracellular particles released from continuous cell cultures derived from human leukemia.

Extracellular particles, with a density of 1.18-1.22 g/cm3 in sucrose, were detected in the culture medium of a continuous cell line (JIII) derived from a patient with monocytic leukemia. These particles contained RNA, DNA, and a DNA polymerase. They synthesized DNA with endogenous templates and primers and also used exogenous DNA but not poly(rC) oligo(dG) as a template. Pretreatment with Nonidet P-40 stimulated DNA polymerase activity while treatment with ribonuclease partially inhibited the enzyme activity. Fluorescent antibodies made to the particles stained both JIII and Z-597 cells derived from human leukemias but not other types of human or nonhuman cultured cells tested. The particles do not appear to be oncornaviruses but may be a particulate antigen associated with malignant cells of hemopoietic and lymphoid origin.

Antibodies, Neoplasm↗
PubMed1976

Occurrence in human bone marrows of an antigen released from continuous cell cultures derived from human leukemia.

Fluorescent antibodies prepared against extracellular particles from a continuous culture of cells derived from a monocytic leukemia stained JIII cells but not cells infected with Rauscher leukemia virus or simian sarcoma virus. These antibodies reacted with 38% of bone marrow preparations from patients with lymphoma, 26% of preparations from patients with nonmalignant blood disorders and 6% of preparations from patients with leukemia. Bone marrow films from patients with lymphoma over the age of 50 stained less frequently than those from patients under 50. These particles released from JIII cells are not antigenically related to two of the commonly studied oncornaviruses, but may be indicative of the etiology or disease process of lymphoma in young patients.

Age Factors↗
PubMed1975

Production of prostaglandin-like substance at the time of mating in the rat.

The amount of prostaglandin-like substance (PG--LS) in the reproductive tract of the male and female rat following mating (p.c.) has been measured and compared with non-mated (control) values. Negligable amounts of PG-LS were detected in the control female tracts but a mean of 2 mug PGE, equivalents was detected immediately p.c. The PG--LS is probably generated in the vas deferens since nearly 1 mug PGE1 equivalents per rat remained there p.c. No other tissue in the male reproductive tract contained significant amounts of PG--LS suggests that the bulk of the activity is due to PGE2 with a smaller amount being due to PGF2 alpha.

Animals↗
PubMed1972

Cross-resistance and collateral susceptibility to antifolic antimalarial compounds.

Series of strains of Streptococcus faecium ATCC 8043, Lactobacillus casei ATCC 7469, and Pediococcus cerevisiae ATCC 8081 with increasing resistance to the active antifolate antimalarial drugs chlorguanide triazine (CGT), pyrimethamine (PM), and trimethoprim (TMP) were isolated. These mutant strains, stable for at least 3 to 5 years, were examined for cross-resistance and collateral susceptibility to the above compounds and to methotrexate (MTX). Generally, they exhibited cross-resistance to all four compounds, but resistance of a strain to one compound did not predict accurately its resistance to another drug. Unexpectedly, L. casei resistant to CGT exhibited collateral susceptibility to MTX, TMP, and PM varying from 5- to 20-fold. P. cerevisiae developed resistance to CGT readily but maintained its susceptibility to PM and TMP after prolonged exposure to these compounds. Resistance to these antimalarial antifolates was accompanied by only low-grade cross-resistance to MTX, a representative antileukemic antifolate agent.

Antimalarials↗
PubMed1972

Infection.

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Anemia↗