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C Breda

Publications and source records attributed to C Breda.

10 recordsLinked to original sources
PubMed1996

Nucleotide sequences of four pathogen-induced alfalfa peroxidase-encoding cDNAs.

We constructed an alfalfa cDNA library from mRNA extracted from leaves after infection with Pseudomonas syringae (incompatible interaction). Screening with oligodeoxyribonucleotides designed from regions conserved in all known peroxidases allowed the isolation of four cDNAs (Msprx1A, 1B, 1C and 2). Sequence analysis revealed the presence of open reading frames of 351, 355, 358 and 323 amino acids, respectively, with the characteristic consensus sequences of plant peroxidases. Sequence comparison showed that the Msprx2 product is significantly different from the others and, particularly, lacks a C-terminal propeptide which might be required for vacuolar targeting.

Amino Acid Sequence↗
PubMed1996

Defense reaction in Medicago sativa: a gene encoding a class 10 PR protein is expressed in vascular bundles.

Infiltration of Medicago sativa leaves with a suspension of Pseudomonas syringae pv. pisi elicits the accumulation of several mRNA classes. A clone, designated as MsPR10-1, encoding a polypeptide exhibiting strong similarity to the class 10 PR protein was isolated and characterized from a cDNA library prepared from leaf mRNA. The corresponding gene was shown to be developmentally regulated: Except in roots, its expression was not detectable in other analyzed organs of healthy plants (hypocotyls, cotyledons, stems, leaves, and flower buds). MsPR10-1 transcript accumulation was especially high in leaf blades during an incompatible interaction: It was already detectable 3 h after infection, reached its maximum level 24 h postinfection, and remained at a high level over a period of at least 72 h. In addition, the expression of this gene was induced by salicylic acid treatment of the leaves. Southern hybridizations showed that this gene belongs to a multigene family. Using a 5' extension technique for cDNA, we demonstrated that during the incompatible interaction with P. syringae pv. pisi several genes or allelic variants of this class were expressed. Measurements of transcript accumulation in both the infiltrated and noninfiltrated zones by Northern and in situ hybridization allowed to demonstrate the "systemic" expression pattern of the MsPR10-1. In situ hybridizations indicated that MsPR10-1 was expressed in the vascular bundles adjacent to and distant from the infection site.

Amino Acid Sequence↗
PubMed1995

[Carcinoma of the lung involving the thoracic wall].

Carcinoma of the lung involving the chest wall causes severe pain. An unusual type of chest wall tumour is Pancoast's carcinoma, also called "the superior sulcus tumour". The long tumour involving the chest wall is considered a T3 lesion and hence stage IIIa. Reconstruction of the chest wall is generally achieved with autogenous tissue; The treatment of a superior sulcus tumour consists of preoperative radiotherapy followed by radical surgical resection of the lung, involving chest wall nerve endings. In Pancoast's tumour 5-year survival is reported as between 23% and 40% with combined irradiation and operation for T3-NO lesions.

Humans↗
PubMed1995

[Indications for and results of video-assisted thoracic surgery].

Between January 1991 and February 1996, and February 1996, 222 videothoracoscopic operations were conducted in 215 patients. Indications for video-assisted thoracic surgery (Vats) have included management of pneumothorax, (91), pleural effusion (59), lung nodules (41), mediastinal biopsy (8), need for lung biopsy (10), small endothoracic masses (6), haemothorax (4), gangliectomy in Raynaud syndrome (1), the removal of jugular vein catheter (1), and middle lobectomy for A-V fistula (1). Fifteen procedures were converted to thoracotomy; nine conversions were made for more extensive resection after a diagnosis of primary lung malignancy. Other reasons for conversion were adhesions (6). There was one complication (haemothorax). There were no intraoperative deaths. Mean operative time was 60 min. (median line); the median chest tube duration was three days and hospital stay five days. The video-assisted thoracic surgery procedures were safe, and for patients the advantages included less pain and earlier post-operative mobilization.

Adolescent↗
PubMed1994

Cloning and expression of a cDNA encoding a cytoplasmic L5 ribosomal protein from alfalfa (Medicago sativa L.).

A cDNA encoding a putative cytoplasmic ribosomal protein L5 from alfalfa (MsRL5), the first sequence from higher plants, has been characterized. The derived amino acid sequence of 181 residues contains the L5 signature, is 72.2% identical to yeast ribosomal L5 and shares high identity with other RL5 peptides from eukaryotic origin. The sequence does not contain any signal or transit peptide and therefore might be cytoplasmic. In all alfalfa organs examined MsRL5 transcripts were detected at approximately equal levels.

Amino Acid Sequence↗
PubMed1994

Bilateral Pancoast syndrome in a patient with metachronous primary lung cancer.

A rare case of double Pancoast tumor in a patient with metachronous primary cancer of the lung was treated with irradiation and operation. Both tumors were managed with pulmonary wedge resection and excision of involved chest wall. Six years after the first operation the patient is doing well without pain and respiratory failure.

Adenocarcinoma↗
PubMed1992

Evidence, from combined segregation and linkage analysis, that a variant of the angiotensin I-converting enzyme (ACE) gene controls plasma ACE levels.

The hypothesis of a genetic control of plasma angiotensin I-converting enzyme (ACE) level has been suggested both by segregation analysis and by the identification of an insertion/deletion (I/D) polymorphism of the ACE gene, a polymorphism contributing much to the variability of ACE level. To elucidate whether the I/D polymorphism was directly involved in the genetic regulation, plasma ACE activity and genotype for the I/D polymorphism were both measured in a sample of 98 healthy nuclear families. The pattern of familial correlations of ACE level was compatible with a zero correlation between spouses and equal parent-offspring and sib-sib correlations (.24 +/- .04). A segregation analysis indicated that this familial resemblance could be entirely explained by the transmission of a codominant major gene. The I/D polymorphism was associated with marked differences of ACE levels, although these differences were less pronounced than those observed in the segregation analysis. After adjustment for the polymorphism effects, the residual heritability (.280 +/- .096) was significant. Finally, a combined segregation and linkage analysis provided evidence that the major-gene effect was due to a variant of the ACE gene, in strong linkage disequilibrium with the I/D polymorphism. The marker allele I appeared always associated with the major-gene allele s characterized by lower ACE levels. The frequency of allele I was .431 +/- .025, and that of major allele s was .557 +/- .041. The major gene had codominant effects equal to 1.3 residual SDs and accounted for 44% of the total variability of ACE level, as compared with 28% for the I/D polymorphism.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1990

Molecular cloning of complementary DNAs encoding two cationic peroxidases from cultivated peanut cells.

We have isolated, cloned, and characterized two cDNAs corresponding to the mRNAs for cationic peroxidases synthesized by cultured peanut cells. The first clone was obtained from a phage lambda gt11 library screened with antibodies directed against the major secreted isozyme. Its predicted amino acid sequence, deduced from the 1228-base-pair (bp) cDNA, revealed a 22-amino acid signal peptide and a 294-amino acid mature protein (Mr, 31,228). The second clone was isolated from a lambda gt10 library screened with oligonucleotides corresponding to the regions for acid/base catalysis and the fifth ligand of heme. This cDNA (1344 bp) encodes a protein (330 amino acids) with a mature peptide of 307 residues (Mr, 32,954). The two peanut peroxidases are 46% homologous. The estimated gene copy numbers of these peroxidases might be close to 1 or 2 per haploid genome. A comparison of the amino acid sequence of these peanut peroxidases with other known isozymes shows two already known regions of homology (the region for acid/base catalysis and the fifth ligand of heme). Moreover, some new characteristics appeared such as a glycosylation site identical in five of the seven isozymes, a putative antigenic determinant common to all the isozymes, and a region of the highest homology. A secondary structure prediction showed that it corresponds to a 16-amino acid helix linked to the next one by a long stretch of beta strands and coils and might represent a critical structural element.

Amino Acid Sequence↗
PubMedDate not supplied

Pathological and molecular characterizations of alfalfa interactions with compatible and incompatible bacteria, Xanthomonas campestris pv. alfalfae and Pseudomonas syringae pv. pisi.

We report on the interactions of alfalfa with Xanthomonas campestris pv. alfalfae and Pseudomonas syringae pv. pisi. A hypersensitive response was observed when leaves were infiltrated with P. s. pv. pisi, which remained strictly limited to the injected zone. The compatible interaction with X. c. pv. alfalfae was characterized by water-soaking symptoms and the spreading of the bacterium into the leaf blade. Analyses of transcript accumulation were conducted with cDNAs encoding enzymes involved in phytoalexin synthesis: chalcone synthase (CHS), chalcone isomerase (CHI), and isoflavone reductase (IFR). In incompatible interactions the maximum accumulation of the CHS, CHI, and IFR transcripts was observed 6 hr postinfection. In the compatible interaction, the induction of these transcripts was delayed until 25-30 hr postinfection, and the level of their accumulation was considerably lower. Extending this molecular analysis to the root system showed that the reaction of roots during an incompatible interaction was quite comparable to that of leaves. To complete these analyses, expression of genes encoding pathogenesis-related (PR) proteins in leaves was also analyzed by polymerase chain reaction. High-level accumulation of a 0.8-kb transcript encoding a PR protein was observed 6 to 30 hr postinfection in the incompatible interaction.

Acyltransferases↗