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Biomedical subjects

C Boulard

Publications and source records attributed to C Boulard.

At least 73 records · Page 4Linked to original sources
PubMed1984

[A comparative study of erythrocytes from 4 animal species in the determination of hemolytic activity in classical and alternative pathways of bovine complement].

The hemolytic activity of bovine complement activated via the classical or alternative pathways was studied employing as target cells, erythrocytes of sheep, rabbit, guinea-pig and chicken. A spontaneous activation of bovine complement via the alternative pathway by rabbit and guinea-pig erythrocytes at a high level and chicken erythrocytes at a lower degree, was observed. Consequently hemolytic activity following activation via the classical pathway reflects activation via the two pathways when erythrocytes of these three species were used. In spite of a low degree of hemolysis, sheep erythrocytes have been chosen for their high specificity in the evaluation of bovine complement activation via the classical pathway. Activation via the alternative pathway can be performed by the erythrocytes of the three other species studied.

Animals↗
PubMed1984

Effect of dose and route of application on the action of decamethrin against cattle grubs.

The efficacy of pour-on or orally administered decamethrin against bovine hypodermosis was investigated on naturally infested yearlings. Treatment at dose rates of 1, 2 or 10 mg per kg body weight were ineffective, but the highest dose had a long-term lethal effect on warbles. The severe side effects which followed the high dose suggest that this drug is unsatisfactory for systemic grub control. Investigations on the dynamics of circulating anti-Hypoderma antibodies in the post-treatment period showed that there is some sub-lethal activity of decamethrin on the larval physiology even at the lowest doses.

Administration, Oral↗
PubMed1984

Changes in the haemolytic activity of bovine serum complement by Hypoderma lineatum (insect oestridae) larval proteinases in naive and immune cattle.

Three serine proteinases (hypodermin A, B and C) of the first instar larvae of Hypoderma lineatum have been assayed for their ability to deplete seric complement of naive or immune cattle. In naive cattle complement consumption is initiated by hypodermin B through the sequence C1-C3 at a concentration of 5 micrograms/ml of serum, and by hypodermin A through the sequence of C3-C9 at a higher concentration of 150 micrograms. The third enzyme presenting a collagenolytic activity has no anti-complementary activity even on C1q. In immune cattle a 70% complement depletion through the classical pathway is observed with 15 micrograms of hypodermin B per ml of serum. The two enzymes A and C appear to play a minor role in the complement depletion via the classical pathway. The biological role of each of these enzymes in the parasite-host interrelationships is discussed. The participation of these enzymes in the immediate hypersensitive reactions following systemic treatment of cattle infested by this endoparasite is considered.

Animals↗
PubMed1984

[Cimetidine treatment of primary hyperparathyroidism].

Since Sherwood's report in 1979, contradictory findings have been published with regards to the treatment of primary hyperparathyroidism with cimetidine. We studied 16 patients with primary hyperparathyroidism treated with 1,200 mg of Cimetidine and followed-up clinically and biochemically. A decrease in serum calcium and urinary CAMP was observed after the fourth week of cimetidine therapy with no significant change in parathormone levels. In none of the cases did Cimetidine seem to provide a treatment of hyperparathyroidism, a condition which remains curable with surgery.

Calcium↗
PubMed1983

[Suppurative thyroiditis and epithelioma of the piriform sinus].

The authors report a case of suppurative thyroiditis revealing cancer of the piriform sinus. The course ran two distinct phases. Initially, symptoms resolved under methylprednisolone and ampicillin given with a diagnosis of acute thyroiditis. After corticosteroids were discontinued, a tumefaction in the thyroid area recurred, with clinical features indicating centesis that removed 30 cc of pus ORL examination connected this suppurative thyroiditis to an epithelioma of a piriform sinus.

Aged↗
PubMed1983

[Attempts at time-related control of bovine hypodermiasis at scattered or grouped farms].

The control was performed by a unic systemic autumn treatment on heifers and repeated topical spring treatments on dairy cows. After a four years programme, limited success are obtained. No difference in the efficacy are observed in isolated or grouped farms. A good estimation of infestation cannot be obtained by only one or two numerations of warbles in spring. Repetitive of topical spring treatments on dairy cows appears to be the major limitating factor of success in these programmes.

Animals↗
PubMed1982

Specific antisera produced by direct immunization with slices of polyacrylamide gel containing small amounts of protein.

Rabbits were injected with slices of polyacrylamide gels containing entrapped insect proteins after separation by electrophoresis. Specific antibodies were produced independently of the nature of the gel (with or without sodium dodecyl sulphate) and of the staining technique (amido black or Coomassie Blue). The procedure appears to be a rapid and simple method for production of antibodies specific to proteins separated in minute quantities from a complex mixture.

Acrylic Resins↗
PubMed1981

Local and systemic reactions in cattle to Hypoderma lineatum larval toxin: protection by phenylbutazone.

Soluble toxin of Hypoderma lineatum injected IV induced a severe cardiorespiratory shock syndrome in calves. Intradermal injection of the toxin caused well-defined, edematous wheals comparable with those produced by histamine. Treatment of calves with famphur (organophosphate insecticide; poured on the skin of the calves 24 hours before injection of toxin) did not increase the severity of the effects of the toxin. Treatment of calves with phenylbutazone (20 mg/kg) 20 minutes before injection of toxin protected the calves against the systemic shock syndrome and the local inflammatory reaction. Apparently, the neurotoxic mechanism of the organophosphate insecticide is independent of the action of the Hypoderma toxin per se. Phenylbutazone can control the effects of the Hypoderma toxin.

Animals↗
PubMed1980

Reaginic (type 1 anaphylactic) antibodies produced by calves in response to Hypoderma larvae.

Reaginic antibody production by Hypoderma lineatum and H bovis larval antigens were evaluated in three calves using passive cutaneous anaphylaxis (PCA). Two calves were sensitised with soluble extract of H lineatum larvae and a third was injected with 43 hatching H bovis larvae. Blood serum taken from the calves was injected intradermally in 0.1 ml volumes into three test calves. Sera from the calves sensitised with hypoderma extract produced positive PCA reactions in all three calves with maximum titres of 1:8. Sera from the calf injected with living larvae caused positive PCA reactions in two of the three test calves, the maximum titre being 1:4. The results indicate that the calves produced reaginic antibody to H bovis and H lineatum, although serum concentrations of the antibody were relatively low.

Animals↗
PubMed1979

Chemical and enzymatic characterization of the collagenase from the insect Hypoderma lineatum.

The collagenase from the larvae Hypoderma lineatum, with a molecular weight of 24 000 and isoelectric point of 4.1, was obtained in homogeneous form by ion-exchange chromatography. It is stoichiometrically inhibited by diisopropylfluorophosphate. On the other hand it is unaffected by ethylenediaminetetraacetate, p-chloromercuribenzoate, dithiothreitol, N-tosyllysine chloromethyl ketone, N-tosylphenylalanine chloromethyl ketone and ovomucoid trypsin inhibitor. The enzyme which degrades native collagen in its helical parts, has a specific activity on thermally reconstituted collagen fibrils of 150 micrograms collagen degraded x min-1 x (mg enzyme)-1 at 37 degrees C. It hydrolyses casein but has no esterolytic activity characteristic of trypsin, chymotrypsin nor elastase. It has no action on the synthetic peptide 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucyl-L-glycyl-L-prolyl-D-arginine. The amino acid composition of Hypoderma collagenase indicates a distinct similarity with the serine proteinases of the trypsin family and with another athropode serine collagenase, that of the fiddler crab Uca pugilator. This suggests that eucaryotic collagenases with digestive rather than morphogenic function represent a new category of members of the trypsin family.

Amino Acids↗
PubMed1977

[Hereditary Sipple syndrome (author's transl)].

This entity, isolated by Gorlin, is characterized by the presence of cutaneous and mucous neuromas, facial disfiguration resembling acromegalia as well as morphological changes to the extremities similar to those produced by Marfan's disease. These factors are also associated with multiple endocrinological neoplasms type II of Sipple's syndrome. In other words, malignant tumors developing from thyroid "C" cells as well as from bilateral benign pheochromocytoma (neoplasias which derive from the A.P.U.D. system). It is considered to be hereditary in nature and transmitted as a dominant trait. Its evolution is triphasic, and the prognosis is extremely dismal. There may exist incomplete or benign forms, in the course of which the endocrinological neoplasms may appear very late or not at all. Generally, the diagnosis is easy. This entity can be calssified with other diseases stemming from the neural ridge, in other words "neurocristopathies" but in spite of these similarities with disease of the same embryological origin, it would seem that this particular entity is genetically autonomous. The only effective treatment is surgical, for thyroid neoplasms, as well as neoplasms occurring in the medullo-adrenal glands. It should be noted that this line of treatment must be undertaken with the utmost precaution.

Adolescent↗
PubMed1976

Testosterone and dihydrotestosterone concentrations in plasma, epididymal tissues, and seminal fluid of adult rats.

The concentration of testosterone (T) and 5alpha-dihydrotestosterone (DHT) in plasma, seminal fluid, and epididymis of male Wistar rats have been determined by radioimmunoassay. The DHT/T ratio rose from 0.1 in blood to 1 in testicular fluid and 4 in epididymal fluid. This ratio is 2 in tissues of "caput" epididymis and 1 in "cauda" epididymis. These data indicate a transformation of T to DHT in the epididymal tissue, particularly at the "caput" level.

Animals↗