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Biomedical subjects

C Boucheix

Publications and source records attributed to C Boucheix.

108 records · Page 6Linked to original sources
PubMed1984

[Monoclonal antibodies against surface antigens of lymphoblasts and blood cells or bone marrow recognize constituents of the human nephron].

By immunizing mice with bone marrow cells of a patient with acute lymphoblastic leukemia, monoclonal antibodies have been produced against differentiation antigens of hemopoietic cells. These antibodies also recognize cells in different parts of the normal human kidney. Two antibodies, ALB1 et ALB2, which recognize the common acute lymphoblastic leukemia antigen, (CALLA), label the podocytes and the epithelial cells of the proximal tubules of the kidney; ALB6, which recognizes the "p 24" antigen of lymphocytes, labels the distal tubules; ALB9, which recognizes lymphoblasts and granulocytes, labels the thick ascending loop of Henle and the collecting tubules; PM1, which recognizes immature granulocytic cells, labels the thick ascending loop of Henle.

Animals↗
PubMed1983

Characteristics of platelet aggregation induced by the monoclonal antibody ALB6 (acute lymphoblastic leukemia antigen p 24). Inhibition of aggregation by ALB6Fab.

The leukemia-associated cell surface antigen p 24 is found on normal platelets as well as on Bernard Soulier syndrome and thrombasthenia type I platelets. ALB6 IgG (a monoclonal antibody against p 24) induces the aggregation of platelets from normal donors but not from thrombasthenia. In contrast, ALB6 Fab inhibits platelet aggregation induced by collagen, ADP, thrombin, ionophore A 23187 and ALB6 IgG. The results suggest that ALB6 interferes with a mechanism common to all aggregation pathways; the possible mechanisms are discussed.

Antibodies, Monoclonal↗
PubMed1983

Detection of binding sites for spiroperidol on leukemic cells: its value for the phenotype characterization of lymphoid leukemias.

The specific binding of the dopamine antagonist spiroperidol was studied in leukemic cell samples of various phenotypes. Among these only B-cell samples from chronic lymphocytic leukemias (7/7) and some "null" cell samples from acute lymphoblastic leukemias (2/7) showed specific binding. B cells from a prolymphocytic leukemia were negative as were also T-lymphoïd and non-lymphoïd leukemic cells at different stages of maturation. This pattern can be clearly correlated with the previous results obtained with normal blood cells and on cell lines. Moreover, it suggests that the detection of spiroperidol binding sites could provide a new means of distinguishing different phenotypes among B cells and early lymphoïd cells. Our results open the way to further studies which might show a correlation between spiroperidol binding sites and the new immunological markers defining subsets among non-T lymphoïd cells, as well as defining their physiological meaning.

Butyrophenones↗
PubMed1982

[Ultrastructural peroxidases and immunological markers in acute adult leukaemia: therapeutic applications (author's transl)].

In a retrospective study of 112 adult patients with acute leukaemia (AL), the percentage of undifferentiated leukaemia was reduced to almost 1% by an analysis of routine cytochemical reactions (PAS, peroxidase, esterases and esterase inhibition), B and T lymphocyte markers (IgS, E-rosettes, HuTLA) cAll antigen and ultrastructural detection of peroxidase activity (PO-ME). A simultaneous study of cALL antigen and PO-ME showed reciprocal exclusion of these markers, except in one case of mixed leukaemia. Therapeutically, the value of these tests lies in that patients with typical PAS reaction and cALL antigen consistently respond to vincristine- corticosteroid treatment, whereas patients without cALL and with PO-ME are frequently resistant to that combination of drugs.

Adult↗
PubMed1982

Lysosomal localisation of parallel tubular arrays in chronic lymphocytic leukaemia of T cell origin: an ultrastructural cytochemical study.

An ultrastructural cytochemical study of lysosomal acid phosphatase was performed on leukemic cells in a case of chronic lymphocytic leukaemia of T cell origin (T-CLL). The cells showed inclusion bodies known as parallel tubular arrays, which often lay within acid phosphatase-positive, membrane-bound spaces. This suggests their lysosomal location.

Acid Phosphatase↗
PubMed1982

Determination of ultrastructural peroxidases and immunologic membrane markers in the diagnosis of acute leukemias.

Detection of membrane markers and ultrastructural peroxidase activity was carried out on the blasts of 16 apparently nonmyeloid adult acute leukemias. These patients were selected from 73 adult leukemic patients by the negativity of their routine cytochemical myeloid markers: i.e., myeloperoxidase, chloresterase activity, and Sudan Black B staining. B and T acute lymphoid leukemias (ALL) were excluded from the study. After concurrent testing from human T lymphocyte antigen (HuTLA), common ALL antigen (cALL), la-like antigens, and peroxidase activity at the electron microscopic level (POEM), only two patients remained undifferentiated (cALL-, POEM-). The other cases were classified as following : 6 common ALL (cALL+, POEM-), 1 pre-T-ALL (cALL+, HuTLA+, POEM-), 5 very poorly differentiated acute myelogenous leukemia (AML) (cALL-, POEM+), and 2 mixed leukemias (cALL+, POEM+). Terminal deoxynucleotidyl transferase activity (TdT) was measured in 7 cases and was found to be present at high levels in 4 cases of cALL and in the 2 cases of acute undifferentiated leukemias (AUL): it was absent in two cases of AML. Cytogenetic analysis had showed that 2 of the cALLs, 3 of the AMLs, and the 2 mixed leukemias were PH1+. We conclude that POEM detection is useful in apparently nonmyeloid leukemias with negative immunologic lymphoid markers, and that the existence of a Ph1 chromosome should be investigated, particularly in the unusual case of mixed (lymphoid-myeloid) acute leukemia.

Acute Disease↗
PubMed1980

Lymphoblastic lymphoma/leukemia with convoluted nuclei: the question of its relation to the T-cell lineage studied in 13 patients.

This work is devoted to the analysis of the nature of lymphoblastic lymphoma/leukemia with convoluted nuclei which were initially described by Barcos and Lukes. Ultrastructural, cytochemical, and immunologic features of tumor cells were investigated in patients chosen according to known morphologic criteria. Through results of the E rosette test, the patients were divided into two groups (E+ and E-). In the E+ group, the predominant features were sex (only men), the mediastinal localization, and the focal positivity of the acid phosphatase reaction. Cytotoxicity tests with rabbit antihuman T-lymphocyte anti-serum confirmed the results of the E rosette test in the 3 patients of the E+ group who were tested and were also positive in 2 patients from the E- group (1 of these 2 patients had the characteristics found in the E+ group and can thus be related to this group; the other patient had none of these characteristics). This raises the question of a leukemia arising from a less differentiated T-cell but this interpretation is limited by the specificity of the anti-T-serum. Ultrastructural study defines more precisely the convoluted aspect but does not at present allow a distinction between the two groups.

Adolescent↗
PubMed1979

Atypical T-cell leukemia terminating Hodgkin's disease.

A case of Hodgkin's disease is described which developed into a terminal illness characterized by a malignant proliferation of T-cells. The leukemic cells, after optical and ultrastructural analysis, were distinct from those of myelomonocytic, acute lymphoblastic, chronic lymphocytic as well as prolymphocytic leukemia. Their relationship with the T-cell lineage seemed to be confirmed by a highly positive E-rosette test and by cytochemistry which showed focal positivity of acid phosphatase. The importance of this T-cell malignant proliferation is discussed, especially with regard to cellular interactions in Hodgkin's disease.

Acid Phosphatase↗
PubMed1978

Human lymphoblastoid cells in culture replicate immune information carried by xenogeneic RNA.

Immune RNA is obtained from lymphoid organs of immunized animals and is reputed to transfer immunological information. Human lymphoblastoid cells in culture, after incubation with sheep Immune RNA produce RNA (Ic-RNA) which carries the same immunological information as the inducing sheep preparation. This Ic-RNA produced in tissue culture is capable of converting 'naive' human lymphocytes to cytotoxic effector cells against tumour target cells, to the same extent as the Is-RNA preparation used for induction of the cell line. The sheep Immune RNA information is present and can be recovered from the lymphoblastoid cells for at least ten weeks after the induction. It is suggested that xenogeneic Immune RNA information is incorporated in a stable fashion by cultured human lymphoblastoid cells, and also that it is replicated during their own replication. This system could be used for studying the incorporation of information carried by exogenous RNA and it might provide insight into some mechanisms underlying the transfer and processing of immunological information.

Cells, Cultured↗
PubMed1977

[Replication of immune RNA by human lymphoblastoid cells].

Animal immune RNA used for the induction of the human lymphoblastoid cell line LDV/7, induces the appearance of receptor sites on the surface of these cells and the synthesis of specific immunoglobulins which are liberated into the culture medium. Furthermore, RNA has been extracted from these cells, which possesses the same properties of specific cytotoxicity as the RNA used for induction. It is supposed that the immune RNA derepresses specific genes on the genome of the induced cells.

Cell Line↗
PubMed1976

In vitro production of a transfer factor specific for transitional-cell carcinoma of the bladder.

Human dialysable Transfer Factor (TFd) extracted from lymphocytes of patients with transitional cell carcinoma of bladder (TCCB) was replicated in culture by lymphoblastoid cell lines. The effectiveness of two such TFdLs produced in vitro in transferring sensitivity to TCCB was assessed in the lymphocyte migration test (LMT) using formalin-treated TCCB cells as antigen. The results, showed that one TFdL transferred sensitivity in 5/14 cases and the other in 12/15, not only to leucocytes of healthy individuals but also to leucocytes of TCCB patients. Preliminary results showing an in vivo transfer of sensitivity are discussed.

Carcinoma, Transitional Cell↗
PubMedDate not supplied

Inhibition of S-antigen-induced experimental autoimmune uveoretinitis by active immunization against anti-idiotypic antibody to an S-antigen epitope.

Experimental autoimmune uveoretinitis (EAU) was induced in rnu/+ rats by one injection of retinal S-antigen (S-Ag) in complete Freund's adjuvant. Immunization of rats, before the S-Ag challenge, with a polyclonal antibody directed at the monoclonal antibody (mAb) S2D2, prevented the development of EAU in rats at clinical and histological levels. Lymph node cells from rats hyperimmunized with this anti-idiotype (anti-Id) S2D2 antibody were stimulated in vitro by anti-Id S2D2, S-Ag, and mAb S2D2. These findings suggest that anti-S2D2 antibody acts as an internal image at the T-cell level for the epitope of S-Ag recognized by mAb S2D2 (epitope S2). From these results, it appears that cells bearing receptors for the internal image of the epitope S2, which is not pathogenic when injected emulsified in adjuvants, are able to inhibit S-Ag-induced EAU.

Animals↗
PubMedDate not supplied

Modulation of experimental autoimmune uveoretinitis by adoptive transfer of cells from rats immunized with anti-S-antigen monoclonal antibody.

Preimmunization of rnu/+ rats with the mouse monoclonal antibody S2D2 against retinal S-antigen (S-Ag) leads to an anti-idiotypic (anti-Id) response and to protection against experimental autoimmune uveoretinitis (EAU) provoked by a subsequent challenge with S-Ag in complete Freund's adjuvant (CFA). Suppression of EAU can be passively transferred using lymph node and/or spleen cells from donors with active anti-Id immunity to naive rnu/+ recipients, prior to immunization with bovine S-Ag in CFA. In contrast, passive administration of IgG from S2D2-immunized rats did not produce suppression. High levels of anti-Id S2D2 antibodies were found: (1) in rats presenting an inhibited EAU after sensitization with S2D2 or after passive transfer of anti-S2D2 cells prior to the S-Ag immunization; and (2) in rats with a severe EAU after transfer of anti-S2D2 IgG associated with anti-S2D2 cells prior to the S-Ag challenge. No effect on the anti-S-Ag antibody level could be detected. These experiments suggest that EAU, a mostly T cell-dependent disease, can be down regulated by anti-Id lymphoid cells.

Animals↗