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Biomedical subjects

C Bouchard

Publications and source records attributed to C Bouchard.

At least 343 records · Page 19Linked to original sources
PubMed1996

Insulin and glucagon responses to adrenaline infusion in abdominal obese men.

OBJECTIVE: To investigate the hormonal, cardiovascular and metabolic responses of visceral vs subcutaneous obese men to infused adrenaline. DESIGN: Intervention study of an hour adrenaline infusion (0.01 micrograms/kg fat-free mass/min). SUBJECTS: Eighteen moderately obese men (age: 30-40 y, BMI: 28-34 kg/m2) divided according to their degree (low vs high) of visceral adipose tissue (AT) accumulation. MEASUREMENTS: Various fatness and fat distribution variables (computed tomography and anthropometry), heart rate and blood pressure, plasma concentrations of gluco-regulatory hormones, glucose, glycerol and free fatty acids (FFA). RESULTS: Similar increases were noted in heart rate, plasma adrenaline, FFA and glycerol levels in both low and high visceral AT groups after hormonal infusion. There was a tendency for plasma glucagon responses to be higher in men with high amounts of visceral fat (p = 0.07). Plasma glucose levels increased in both groups, but significantly more in men with low levels of visceral AT (p < 0.05), whereas plasma insulin concentrations increased significantly only in men with high amounts of visceral AT in response to adrenaline infusion (p < 0.01). In the overall sample of obese men, visceral AT accumulation (but not the fat mass) was positively correlated with plasma insulin (r = 0.70) and glucagon responses (r = 0.63) to the hormone infusion (p < 0.01). These metabolic responses were not related to the achieved catecholamine concentration. CONCLUSION: These results suggest that some of the impairments in plasma glucose-insulin homeostasis noted in visceral obesity may be related to an abnormal metabolic response to an adrenaline challenge.

Abdomen↗
PubMed1996

Associations between 12 year changes in body fatness and lipoprotein-lipid levels in men and women of the Québec Family Study.

OBJECTIVE: To investigate the associations between 12 year changes in body composition, subcutaneous fat distribution vs changes in plasma lipoprotein-lipid levels. DESIGN: 12 year prospective study. SUBJECTS: A sample of 95 women and 93 men of the Québec Family Study initially tested in 1980. MEASUREMENTS: Various body fatness variables as well as fasting plasma lipoprotein-lipid concentrations performed both in 1980 and 1992. RESULTS: In both 1980 and 1992, a high body fat mass and an elevated accumulation of subcutaneous trunk fat were associated with a significant deterioration in the plasma lipoprotein-lipid profile. Furthermore, correlation analysis performed on differences noted during the 12 years follow-up revealed significant associations between changes in body fat mass and in plasma cholesterol [r = 0.52, P < 0.0005] in women. In both men and women, an increased body fat mass was associated with an increased CHOL/HDL-cholesterol ratio [r = 0.37, P < 0.01 (men) and r = 0.54, P < 0.0005 (women)]. Correlations between changes in fat mass and plasma lipids were generally of higher magnitude in women than in men. Changes in subcutaneous trunk fat were associated with changes in plasma HDL-chol levels (r = -0.22, P < 0.05) in men whereas in women, changes in trunk adiposity were related to changes in both plasma CHOL (r = 0.25, P < 0.05) and TG (r = 0.32, P < 0.005) levels. CONCLUSION: These results support the notion that the increased adiposity observed among aging adult men and women is a significant component of the deterioration in the plasma lipoprotein-lipid profile noted over a 12 year follow-up period.

Adipose Tissue↗
PubMed1995

A transforming growth factor beta-like immunosuppressive factor in immunoglobulin G-binding factor.

Immunoglobulin G-binding factors (IgG-BF), which are produced by cells of the immune system, inhibit antibody production. In this paper, we show that transforming growth factor-beta (TGF-beta) suppresses secondary in vitro anti-sheep red blood cell responses of mouse splenocytes and lipopolysaccharide- or anti-IgM-stimulated mouse B cell responses in a way similar to, and with the same kinetics as, rodent IgG-BF. Moreover, the immunosuppressive activity of IgG-BF was totally neutralized by polyclonal and monoclonal anti-TGF-beta antibodies and it eluted with TGF-beta by gel exclusion chromatography, suggesting that a TGF-beta-like immunosuppressive factor is present in IgG-BF. We also show that TGF-beta behaves as an IgG-BF since it binds to insolubilized IgG, but not to insolubilized F(ab')2 or bovine serum albumin. Altogether, the data support the concept of a biological role for TGF-beta in the IgG-mediated negative feedback of antibody responses.

Animals↗
PubMed1995

Physical activity and public health. A recommendation from the Centers for Disease Control and Prevention and the American College of Sports Medicine.

OBJECTIVE: To encourage increased participation in physical activity among Americans of all ages by issuing a public health recommendation on the types and amounts of physical activity needed for health promotion and disease prevention. PARTICIPANTS: A planning committee of five scientists was established by the Centers for Disease Control and Prevention and the American College of Sports Medicine to organize a workshop. This committee selected 15 other workshop discussants on the basis of their research expertise in issues related to the health implications of physical activity. Several relevant professional or scientific organizations and federal agencies also were represented. EVIDENCE: The panel of experts reviewed the pertinent physiological, epidemiologic, and clinical evidence, including primary research articles and recent review articles. CONSENSUS PROCESS: Major issues related to physical activity and health were outlined, and selected members of the expert panel drafted sections of the paper from this outline. A draft manuscript was prepared by the planning committee and circulated to the full panel in advance of the 2-day workshop. During the workshop, each section of the manuscript was reviewed by the expert panel. Primary attention was given to achieving group consensus concerning the recommended types and amounts of physical activity. A concise "public health message" was developed to express the recommendations of the panel. During the ensuing months, the consensus statement was further reviewed and revised and was formally endorsed by both the Centers for Disease Control and Prevention and the American College of Sports Medicine. CONCLUSION: Every US adult should accumulate 30 minutes or more of moderate-intensity physical activity on most, preferably all, days of the week.

Adult↗
PubMed1995

Major gene influence on the propensity to store fat in trunk versus extremity depots: evidence from the Québec Family Study.

Regional fat distribution is related to higher risks of cardiovascular morbidity and mortality, independent of general obesity. In particular, a centralized pattern of fat deposition, characterized by greater abdominal stores relative to extremity stores, is associated with a higher propensity to metabolic complications. Motivated by these considerations, we have initiated a systematic investigation of several measures of regional fat distribution aimed at the identification of possible major gene effects. Two measures approximate the size of subcutaneous fat stores: the sum of six skinfold thicknesses (SF6 = abdominal + suprailiac + subscapular + calf + triceps + biceps), and the sum of three trunk skinfold thicknesses (TSF3 = abdominal + suprailiac + subscapular). Both of these phenotypes are highly correlated with total fat mass, 0.83 and 0.78 for SF6 and TSF3, respectively. The trunk to extremity ratio [TER = TSF3/ (calf + triceps + biceps)] is perhaps the most important of these phenotypes insofar as it is an index of centralized obesity; it is modestly correlated with fat mass (r = 0.18). Each of these phenotypes was adjusted for total fat mass by regression prior to analysis so that we could examine genetic effects on these measures of regional fat distribution without the confounding influence of the determinants of fat mass itself. Segregation analysis of SF6 and TSF3 controlled for total fat mass suggests the presence of a major effect underlying the observed phenotypic distribution; however, tests on the transmission probabilities did not substantiate the segregation of a Mendelian gene.(ABSTRACT TRUNCATED AT 250 WORDS)

Adipose Tissue↗
PubMed1995

Skeletal muscle metabolism and body fat content in men and women.

The purpose of the present study was to verify the relationships between indicators of body fat content and specific characteristics of skeletal muscle in a large sample of men and women. Six skinfold thicknesses (sigma 6S) and maximal oxygen uptake (VO2 max) were measured in 348 Caucasian subjects (149 women and 199 men). Fiber type proportions (type I, type IIA, and type IIB) and activity levels of marker enzymes for the Krebs cycle (malate dehydrogenase, MDH) and for the fatty acid oxidation (3-hydroxyacyl CoA dehydrogenase, HADH) pathways were determined in vastus lateralis muscle samples. No significant correlation was found between fiber type proportions and sigma 6S. Significant and negative correlations were, however, obtained in both genders between the sigma 6S and MDH enzyme activity (r = -0.23; p < 0.01), but not between the sigma 6S and HADH enzyme activity. When individuals with low and high amount of subcutaneous fat but paired for VO2max were compared, vastus lateralis of fat men exhibited the same proportion of type I fiber (38.6 +/- 10.3 vs 38.5 +/- 13.4%) and HADH activity level (3.43 +/- 1.05 vs. 3.34 +/- 0.81 U/g), but had about 20% less MDH enzyme activity than vastus lateralis of leaner men (158 +/- 35 vs. 198 +/- 43 U/g;p < 0.05). No difference was found in any of these muscle phenotypes when comparisons were made between women with low and high amount of subcutaneous fat but also paired for VO2max. Moreover, no relations were observed between skeletal muscle fiber type proportion or metabolic markers with relative subcutaneous fat distribution.(ABSTRACT TRUNCATED AT 250 WORDS)

3-Hydroxyacyl CoA Dehydrogenases↗
PubMed1995

DNA polymorphisms in the alpha 2- and beta 2-adrenoceptor genes and regional fat distribution in humans: association and linkage studies.

The aim of this study was to investigate the relationships between DNA restriction fragment length polymorphisms (RFLP) in the alpha 2- and beta 2-adrenoceptor genes and body fat distribution in humans. Skinfold thickness measurements and genetic analyses (Southern blot) were performed on 280 individuals (142 parents and 138 offsprings) from the Québec Family Study. Using the association study design in unrelated adults, women but not men carrying the 6.3-kb allele of an alpha 2A-adrenoceptor/DraI RFLP had a significantly higher trunk to extremity skinfold ratio (= sum of subscapular+suprailiac+abdominal skinfolds/sum of biceps+triceps+medial calf skinfolds) compared to women without the allele (1.44 +/- 0.52 vs. 1.12 +/- 0.33; p < 0.005 after adjustment for age, p < 0.002 after adjustment for age and body mass index or for age and subcutaneous fat). Using the sib-pair linkage procedure, a significant inverse relationship was found between the proportion of alleles identical by descent shared by sibs at the alpha 2A RFLP marker locus and the squared differences of the trunk to extremity skinfold ratio (p = 0.02 after adjustment for age or for age and body mass index or for age and subcutaneous fat). For a beta 2-adrenoceptor/BanI RFLP, no significant association or linkage was found between fat distribution indicators and the marker. These results suggest that alpha 2A-adrenoceptor gene variability detected with DraI is associated with a relative subcutaneous fat pattern favoring accumulation of truncal-abdominal fat in women, and that the alpha 2A-adrenoceptor gene, or a locus in close proximity, may be linked to body fat distribution in humans independently of the overall level of fatness.

Adipose Tissue↗
PubMed1995

Do elevated levels of abdominal visceral adipose tissue contribute to age-related differences in plasma lipoprotein concentrations in men?

Age-related differences in body fat and more specifically in the accumulation of abdominal visceral adipose tissue were examined as a potential covariate for the alteration in the plasma lipoprotein profile found with aging. For that purpose, results from 79 young adults (aged 24.5 +/- 4.0 years) were compared to 61 middle-aged men (54.7 +/- 6.4 years). Younger men had significantly lower body fat mass and abdominal visceral adipose tissue area measured by computed tomography than middle-aged men (P < 0.0001). Plasma cholesterol, triglyceride, apolipoprotein (apo) B, low density lipoprotein (LDL)-cholesterol and LDL apo B levels, as well as the ratio of plasma cholesterol to high density lipoprotein (HDL)-cholesterol were all significantly lower in younger men as compared to middle-aged men (P < 0.0001). The comparison of younger men to middle-aged men with comparable levels of abdominal visceral fat and total body fat eliminated the age-related differences in plasma triglyceride and in the ratio of plasma cholesterol to HDL-cholesterol, whereas the difference in plasma apolipoprotein B levels, although still significant, was largely reduced. Age-related differences in plasma cholesterol and in LDL-cholesterol levels were still observed after this matching procedure and the differences between age-groups were essentially of similar magnitude than before pairing. In summary, these results suggest that the deterioration of plasma lipoprotein profile observed in middle-aged men as compared to young adult men is partly mediated by concomitant increases in total body fat and abdominal visceral adipose tissue. However, other factors related to the aging process appear to be involved, particularly for the age-related increase in plasma LDL-cholesterol.

Adipose Tissue↗
PubMed1995

Does lipoprotein or hepatic lipase activity explain the protective lipoprotein profile of premenopausal women?

Numerous studies have reported that women have a lipoprotein profile suggestive of a reduced risk of coronary heart disease (CHD). We have therefore tested whether the "protective" lipoprotein profile of women could be explained by differences in hepatic lipase (HL) or lipoprotein lipase (LPL) activities. In the present study, 14 non-obese healthy premenopausal women had higher plasma concentrations of high-density lipoprotein cholesterol (HDL-C), HDL2-C, HDL3-C, and HDL-apolipoprotein (apo) AI, and a higher ratio of HDL-C to low-density lipoprotein cholesterol (LDL-C) than 17 non-obese healthy men. Women also had lower plasma triglyceride (TG), HDL-TG, and apo B levels than men. Plasma postheparin LPL (PH-LPL) and HL activities showed no significant sex dimorphism, whereas abdominal and femoral adipose tissue (AT)-LPL activities were significantly higher in women (P < .005). In men, PH-LPL activity correlated significantly with plasma HDL2-C (r = .52, P < .05), LDL-C (r = -.47, P < .05), and apo B (r = -.56, P < .01) levels, as well as with the HDL-C/LDL-C ratio (r = .67, P < .005). No such relationships were found in women, with the exception of HL activity, which was negatively correlated with HDL-apo AI levels. In both genders, abdominal AT-LPL activity showed no significant association with plasma lipoprotein levels.(ABSTRACT TRUNCATED AT 250 WORDS)

Adipose Tissue↗
PubMed1995

Plasma glucose, insulin, and glucagon before and after long-term overfeeding in identical twins.

Plasma glucose, insulin, and glucagon levels were measured before and after long-term overfeeding (4.2 MJ/d during a 100-day period) in 24 lean adults (12 pairs of monozygotic twins). Fasting plasma glucose, insulin, and glucagon were significantly increased by overfeeding. During a 75-g oral glucose tolerance test (OGTT), no major alteration in glucose tolerance was observed and insulin area under the curve was increased. During a meal test, insulin and glucagon areas under the curve were increased. The pre-overfeeding values for glucose, insulin, and glucagon (fasting and areas) were not correlated with the gains in body weight and in fat mass. However, fasting glucagon before overfeeding was positively correlated with the gains in abdominal visceral fat and in femoral fat. The changes with overfeeding in insulin area during the OGTT were positively correlated with the changes in total subcutaneous fat, even after adjustment for total body fat gain. Significant twin intrapair similarity was observed for fasting plasma glucagon before overfeeding and for the changes in fasting insulin and glucagon with overfeeding. These results indicate that (1) in response to long-term overfeeding, both fasting insulin and glucagon are increased; (2) initial levels of glucose, insulin, and glucagon do not predict the gains in body weight and total body fat during overfeeding, but are related to changes in indicators of fat topography; (3) the changes in total subcutaneous fat represent an important correlate of insulin changes with overfeeding; and (4) the genotype could be an important determinant of insulin and glucagon responses to a prolonged positive-energy-balance period.

Adipose Tissue↗
PubMed1995

Exercise and energy intake: effect of substrate oxidation.

The main objective of this study was to evaluate the short-term effect of exercise-induced alteration in fat oxidation on postexercise spontaneous energy and macronutrient intakes. Eleven young males were submitted to two randomly assigned sessions of 48 h each, during which they were requested to eat in the laboratory. One of these sessions was preceded by a 90-min exercise bout at an intensity of 60% VO2max. During both sessions, subjects ate ad lib food with a fat content conforming to the recommendations of nutrition agencies with a food quotient (FQ) > or = 0.85. Results showed that there was no significant change in postexercise energy and macronutrient intakes in comparison with the sedentary session. However, when subjects were subdivided into two groups on the basis of the respiratory quotient (RQ) measured during exercise, men with a low RQ (high fat oxidation) were characterized by a reduced postexercise increase in energy intake relative to the energy cost of exercise (ECE), i.e., they were more predisposed to be in negative postexercise energy balance compared to those exhibiting a high RQ. Accordingly, exercise RQ was positively associated with postexercise energy and lipid balance. These results show that postexercise energy balance partly depends on the composition of the substrate mix oxidized during exercise.

Adult↗
PubMed1995

Ligands and biological activities of soluble Fc gamma receptors.

Soluble forms of low-affinity Fc gamma receptors (sFc gamma R) circulate in biologic fluids. Their plasmatic levels vary in immunological disorders or related diseases. They are produced by enzymatic cleavage of the membrane receptors or by alternative splicing. They bind IgG with the same isotype specificity as their cell surface counterparts and thus modulate Fc-dependent immune functions. Recent data suggest that they also bind non-Ig ligands present on leukocytes. Functional implications of these findings are discussed.

Animals↗
PubMed1995

Alteration of serotonin release in the guinea pig orbito-frontal cortex by selective serotonin reuptake inhibitors. Relevance to treatment of obsessive-compulsive disorder.

Potent serotonin (5-HT) reuptake inhibitors are the only antidepressant agents thus far shown to be effective in the treatment of obsessive-compulsive disorder (OCD). Positron emission tomography studies in humans have implicated the orbito-frontal cortex and the head of caudate nucleus in the mediation of OCD symptoms. Since the delay of the maximal therapeutic effect of selective 5-HT reuptake inhibitors (SSRI) is longer in OCD than in major depression and the terminal 5-HT autoreceptor is not desensitized in the guinea pig frontal cortex after 3 weeks of SSRI administration, the effects of the SSRI paroxetine (10 mg/kg/day) and fluoxetine (5 mg/kg/day) on 5-HT release and on the sensitivity of the terminal 5-HT autoreceptor were investigated in the guinea pig frontal cortex, the orbito-frontal cortex, and the head of caudate nucleus following a washout period after 3 and 8 weeks of treatment. In preloaded slices prepared from guinea pigs treated with paroxetine for 3 weeks, the electrically evoked release of [3H]5-HT release was enhanced in the frontal cortex (21%) but not in the orbito-frontal cortex or in the head of caudate nucleus. However, after an 8-week treatment, the evoked release of [3H]5-HT was significantly enhanced in the orbito-frontal cortex (55%) and in the rest of the frontal cortex (29%) from the same animals, but still unchanged in the head of caudate nucleus. Concentration-effect curves, constructed with the 5-HT autoreceptor agonist 5-methoxytryptamine, showed that the terminal 5-HT autoreceptor was desensitized only in the orbito-frontal cortex after 8 weeks of treatment with paroxetine. Furthermore, the 5-HT transporter was desensitized in the frontal cortex but not in the orbito-frontal cortex. In the case of 3- or 8-week fluoxetine treatment, neither [3H]5-HT release nor the sensitivity of the terminal 5-HT autoreceptor were altered in the orbito-frontal cortex and the head of caudate nucleus. This could be attributable to a smaller degree of 5-HT reuptake inhibition achieved with fluoxetine, in keeping with the notion that higher doses of SSRI are generally required to improve OCD than depression. Taken together, these results indicate that, in the orbito-frontal cortex, the enhanced release of [3H]5-HT induced by prolonged and marked 5-HT reuptake inhibition is attributable to a desensitization of the terminal 5-HT autoreceptor.

Animals↗
PubMed1995

The genetics of obesity: from genetic epidemiology to molecular markers.

Obesity is a highly prevalent disease that carries enormous human and economic costs in western nations. The complexity and diversity of the paths leading to an overweight or an obesity status are enormous. The etiology, causes, associated morbidity, treatment, benefits versus risks of weight loss, prevention, and other aspects of obesity are all highly complex and intimately associated with other diseases, the prevalence of which is augmented by our present way of life. This article gives a brief overview of the current status of knowledge of the genetic basis of human obesity from a genetic epidemiology, experimental genetic and molecular biology perspective. It appears likely that the susceptibility to obesity depends, to a large extent, on several autosomal genes.

Agouti Signaling Protein↗
PubMed1995

Hyperinsulinemia and regulation of energy balance.

Recent data suggest that hyperinsulinemia with euglycemia may favor the restoration of energy balance when one gains body weight. To test the validity of this concept in humans, the data of 24 young men who had been exposed to a 353-MJ overfeeding protocol for 100 d and who were remeasured after a 4-mo follow-up were analyzed. The sample was subdivided in two groups on the basis of the overfeeding-induced change in postprandial plasma insulin. The increase in postprandial energy expenditure induced by overfeeding was significantly greater in high than in low postprandial insulin responders (P < 0.05) but not after body fat gain was controlled for. After the overfeeding protocol, the loss of subcutaneous adiposity was greater in high than in low postprandial insulin responders. However, this difference was no more significant after the gain in fat mass was controlled for. There was no difference between the two groups in post-overfeeding loss of body weight, fat mass, or fat-free mass. These results partly agree with the idea that hyperinsulinemia resulting from a long-term positive energy balance and its associated fat gain exert a regulatory role promoting the restoration of energy balance.

Adipose Tissue↗
PubMed1995

Alcohol and a high-fat diet: a combination favoring overfeeding.

The effects of alcohol and dietary fat on spontaneous energy and macronutrient intakes were investigated in eight male subjects who participated in a protocol including four randomly assigned 2-d sessions during which they ate ad libitum. In each session they had free access to either high- or low-fat foods, with alcohol or a placebo. The high-fat diet was associated with a substantial increase in daily energy intake. Alcohol had no inhibitory effect on food intake and its energy content was thus associated with an additional increase in energy intake. The enhancing effects of alcohol and dietary fat on daily energy intake were additive so that overfeeding was maximal (2.8 MJ/d) under the high-fat diet+alcohol condition. To further examine the effects of alcohol on energy and macronutrient intakes, reported food intake was studied in 351 men and 360 women who had been tested in the Québec Family Study. The results showed that a high alcohol intake was associated with a high daily energy intake and had no inhibitory effect on lipid intake. In conclusion, a dietary regimen providing a high fraction of energy as alcohol and fat increases the risk for positive energy balance under free-living conditions.

Adult↗
PubMed1995

Genetic determinism of fiber type proportion in human skeletal muscle.

Skeletal muscle fiber type distribution is quite heterogeneous, with about 25% of North American Caucasian men and women having either less than 35% or more than 65% of type I fiber in their vastus lateralis muscle. To what extent human skeletal muscle fiber type proportion is under the control of genetic factors is examined in this paper. The results summarized here suggest that about 15% of the total variance in the proportion of type I muscle fibers in human is explained by the error component related to muscle sampling and technical variance, that about 40% of the phenotype variance is influenced by environmental factors, and the remaining variance (about 45%) is associated with inherited factors. These estimates suggest that a difference of about 30% in type I fibers among individuals could be explained exclusively by differences in the local environment and level of muscular contractile activity. However, unidentified genetic factors would have to be invoked to account for the observation that the skeletal muscle of about 25% of the North American Caucasian population have either less than 35% or more than 65% of type I fibers.

Analysis of Variance↗