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C Bona

Publications and source records attributed to C Bona.

240 records · Page 14Linked to original sources
PubMedDate not supplied

[Specific blast transformation of rabbit spleen cells induced by a bacterial polysaccharide and inhibitory effect of peripheral blood lymphocytes (author's transl)].

Following immunization with bacteria (i.e. Salmonella johannesburg), rabbit spleen lymphocytes developed a specific blast response when the lymphocytes were stimulated with polysaccharide, the haptenic moiety of lipopolysaccharide. A clear cut dissociation was noted in the blast response induced by polysaccharide compared with those induced by lipopolysaccharide and lipid A. There was no correlation between the magnitude of the cellular responses and that of the antibody response. Moreover, there was less specificity at the cellular level than at the level of antibody secreted by cells. A decrease of 3H-thymidine incorporation was often observed after immunization, at the level of peripheral blood lymphocytes. An inhibitory effect of these cells was shown on the blast response of spleen lymphocytes with polysaccharide. A high blast response to Salmonella polysaccharide which could be observed in some non-immunized rabbits might be related to a natural sensitization of animals with the same or related unknown antigens which could not be recognized by anti-S. johannesburg antibodies.

Animals↗
PubMedDate not supplied

[Isolation and characterization of mitogens from Nocardia].

Two methods have been devised to isolate mitogens from Nocardia cells. (1) Lysozyme treatment of delipidated cells followed by fractionation of the soluble extract leads to a mitogen devoid of cell wall constituents. (2) Cell disruption followed by differential centrifugations shows that two mitogenic structures are present: the cell wall and fragments of the cytoplasmic membrane. The active component of the cell wall is the peptidoglycan wherefrom soluble mitogenic fractions have been obtained using bacteriolytic enzymes other than lysozyme. An intact glycan moiety seems necessary for mitogenic activity.

Centrifugation↗