Glyoxysomes isolated from castor bean (Ricinus communis L. var. zanzibariensis) endosperm have been stained by the cytochemical diaminobenzidine reaction. The reaction product obtained by preincubation with 3,3'-diaminobenzidine and incubation with the reagent and H(2)O(2) is distributed uniformly throughout the matrix of the organelles. Ricinosomes or dilated cisternae may be completely absent from the organelle preparation or are, at the most, a minor contaminant.

Journal Article↗

PubMed1974

[Membranes of glyoxysomes from castor bean endosperm: further investigations on the membrane bound enzymes of fatty acid degradation and of the glyoxylate cycle].

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Ricinus communis↗

PubMed1973

Membranes of glyoxysomes from castor-bean endosperm. Enzymes bound to purified-membrane preparations.

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Alcohol Oxidoreductases↗

PubMedDate not supplied

The role of intraoperative quick PTH measurements in primary hyperparathyroidism.

BACKGROUND: In patients with primary hyperparathyroidism (PHPT), circulating concentrations of intact parathyroid hormone (PTH), decline dramatically within minutes following surgical excision of hyperfunctioning parathyroid tissue. The magnitude of this decay correlates with the completeness of resection of hyperfunctioning parathyroid tissue and can be monitored during the operation. METHOD: Intraoperative Quick PTH (QPTH) monitoring and pitfalls of more than 350 patients, who were operated because of primary hyperparathyroidism are analyzed. Special attention is given to correct baseline values and interpretation of QPTH values. RESULTS: QPTH monitoring is able to distinguish reliably between single and multiple gland disease and is an indispensable prerequisite for any form of limited parathyroid exploration. Experience with QPTH monitoring is necessary to achieve the excellent results known from bilateral neck exploration. CONCLUSION: Applying correct baseline values and cautious interpretation of QPTH values results in excellent results. Nevertheless more data must be collected to allow reliable interpretation of QPTH monitoring in all patients with PHPT.

Humans↗

PubMedDate not supplied

[Hormonal pregnancy parameters in the serum following surgery for extrauterine pregnancy].

We did serial determinations of beta-HCG, total-HCG, prolactin, SP-1, and progesterone in maternal serum after operation of an ectopic pregnancy in 38 patients. Prolactin, progesterone and SP-1 rapidly returned to normal values after removal of the ectopic pregnancy. However, the half life of HCG was related to the type of operation: after conservative operation of ectopic pregnancy the half life of HCG was greater than after tubectomy (41.4 +/- 1.9 SEM hours versus 33.4 +/- 2.0 hours), possibly due to lack of complete removal of trophoblast tissue. This fact should be kept in mind when evaluating postoperative HCG levels.

Adult↗

PubMedDate not supplied

[A fast (3 minutes) latexagglutination inhibition test for semiquantitative determination of urinary estriol in pregnancy (author's transl)].

Urine samples from 176 healthy women between 29th and 40th week of gestation were analyzed by a test kit. Estrotec Slide Test, which offers a simple and fast test performance for estriol in pregnancy urine. The mechanism of this test system is based on a competitive latex agglutination inhibition reaction. The correlation between Estrotec Slide Test and estriol levels assayed in the same urine samples by a chemical method was r = 0,83. Variation of estriol determinations from urine and estriol levels in serum samples of women at the end of gestation measured by a radioimmunologic method was about 20% for all test principles examinated. The Estrotec Slide Test was proved to be a fast, semiquantitative method. The simple assay procedure itself imposes limits to the information available from it.

Estriol↗

PubMedDate not supplied

Tissue polypeptide-specific antigen (TPS) in pediatric malignancies.

Tissue Polypeptide Specific Antigen (TPS) may soon be routinely used as a proliferation marker in adult epithelial tumors. So far, no data have been available on normal or pathologic TPS values in children. Therefore the present study was designed to test the marker for the first time in pediatric malignancies. Using a commercial ELISA kit (Beki Diagnostics), serum TPS levels were determined in 270 healthy children and compared with various benign (n = 143) and malignant (n = 58) diseases. In healthy children, we found an age-dependent distribution of TPS values. Median (M) TPS was found to be 105.05 U/l at birth as determined from umbilical cord blood (n = 96). By the end of the first week, the value rose to M = 164 U/l and then continuously decreased with age until reaching the adult level at around 14 years. Patients with benign masses (n = 29) did not show elevated serum TPS in contrast to children with malignancies. Advanced tumor stage, metastases, and low dignity correlated with an increase in serum values. In many cases, chemotherapy and especially surgical resection of the tumor were followed by a decrease of the previously markedly elevated TPS levels. Severe infections and impaired renal function were however related with very high values and this must be taken into account when judging the validity of TPS measurements. The marker appears useful for the differential diagnosis between benign and malignant processes. While previous investigators described its use exclusively in epithelial tumors of adults, we cannot confirm this specifity in pediatric patients: Our data show that elevated TPS values in children can also be observed in nonepithelial tumors.

Adolescent↗