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Biomedical subjects

C Berger

Publications and source records attributed to C Berger.

At least 307 records · Page 17Linked to original sources
PubMed1977

[Dialysis-computer program. IV. Summary report. Epidemiology of complications].

The Diaphane-program instituted under the authority of the French Society of Nephrology has been steadily expanding since 1972. By December 1977, about 1500 patients treated in 30 public and private Dialysis Centres were followed up by this system. Full coverage of expenses is provided by the participating Centres. The statistical work presented in this report involves 1572 adult patients treated between June 1972 and December 1976 in 24 dialysis centres. The amount of collected data and the duration of the observation period permit to build up evolutive profiles of the population of patients treated in France by maintenance hemodialysis, of the various techniques and strategies used and of the main complications recorded in the patients. 1. Mean age of patients at start of dialysis is steadily increasing, from 40.1 years in 1972 to 48.2 years in 1976. 2. The predominance of male patients, constant over each year, may be explained by an increased proportion in man of chronic glomerulonephritis and renal vascular diseases. The sex-ratio in patients with chronic pyelonephritis is close to the one recorded in the French population. 3. The regular decrease of the mean plasma creatinine level at time of first dialysis recorded since 1972, is probably related to an earlier start of treatment. However, 10.6 per cent of the patients taken on treatment in 1975-1976 still had a plasma creatinine greater than or equal to 200 mg/100ml. 18.7 per cent had a diastolic blood pressure greater than or equal to 120 mmHg, and exsudative lesions at eye fundi examination were found in 33.5 per cent. The delay in initiating dialysis treatment may account for the frequency of early acute cardiopulmonary complications such as pulmonary oedema and pericarditis and also for the increase in the mortality rate recorded during the first year of treatment: 12.1 per cent instead of 6.2 per cent during the second year. This particularly relevant for the younger age group of patients. 4. There seems to be some social disparity concerning the detection of renal disease and the conditions under which dialysis treatment is started: chronic renal disease is detected at an earlier stage and dialysis treatment initiated for lower values of plasma creatinine and of diastolic blood pressure in patients belonging to the "higher income" group of population. 5. The percentage of patients dialysed twice a week is steadily increasing, whereas the average weekly dialysis time decreases, being about 15 hours in 1976. Day and evening dialysis replace overnight dialysis. Disposable flat-plate dialysers are used increasingly. 6. Episodes of hypotension and cramps are the incidents most frequently recorded during the dialysis sessions. Risk factors evidenced in the occurrence of hypotensive accidents are: the female sex, age greater than or equal to 55 years in males, orthostatic blood pressure drop at the end of previous dialysis, weight loss of more than 4 per cent of total body weight during dialysis...

Adult↗
PubMed1977

[Distribution of birth weights and influenza epidemics (author's transl)].

The possible consequences of influenza during pregnancy on the weight of the new born were studied. It was shown that the major 1969-70 winter influenza epidemic in Alsace was followed by a decrease in the mean birth weight registered in Haguenau's maternity hospital. This study confirms previous work on the subject and shows that birth weight can be used as a valuable tool at the epidemiologic scale.

Birth Weight↗
PubMed1976

CHRONOS: a data base management package for physicians and researchers.

This paper describes a general data base management package defined for medical applications. CHRONOS is a user-oriented system which has been designed for physicians to get periodical reports and for researchers to prepare statistical treatments. The basic principles of the data base and program organization are described: many possibilities are offered for data acquisition and specific efforts have been made in order to analyze easily the evolution of patients. Several medical applications are now operational with CHRONOS in fields as different as psychiatry and nephrology.

Computers↗
PubMed1976

Amino-acid sequence of equine renal metallothionein-1B.

The amino-acid sequence of a metallothionein is reported. Metallothionein is a widely distributed, extremely cysteine-rich, low-molecular-weight protein containing large amounts of cadmium and/or zinc. Metallothionein-1B is one of the two prinicipal variants occurring in equine kidney cortex. The single-chain protein contains 61 amino acids and has the composition Cys20 Ser8Lys7Arg1Ala7Gly5Val3Asp2Asn1-Glu1Gln2Pro2Thr1Met1(Cd + Zn)7. Its amino-terminal residue is N-acetylmethionine. The sequence shows distinct clustering of the twenty cysteinyl residues into seven groups separated by stretches of at least three other residues. Within these groups the cysteines occur seven times in alternating Cys-X-Cys sequences and three times each in Cys-Cys and Cys-X-X-Cys sequences, where X is an amino acid other than cysteine. Another unique feature is the close association of serine and the basic amino acids with cysteine, as manifested by the occurrence of seven Ser-Cys, four Cys-Lys, one Cys-Arg, and three Lys-Cys sequences. These findings are in agreement with the previous suggestion that metallothionein has structurally defined metal-binding sites, most of which contain three cysteinyl residues as the principal metal-binding ligands. The charge difference between the metal-free and the metal-containing protein is consistent with the formation of negatively charged trimercaptide complexes with cadmium and/or zinc ions. The possible additional involvement of serine and the basic amino acids in metal binding is discussed.

Amino Acid Sequence↗
PubMed1976

[Peculiarities of hemostasis in the term newborn infant].

Haemostasis in the new-born is a product of various factors which are both qualitative and quantitative. The only factors that compare in levels and quality with those in the adult are factors V, VIII and XIII. They are alterations in the semi-analytical tests for coagulation except in the Stypven time. In contrast with this deficit shown up in haemostasis by global tests coagulation is normal and in truth there is hypercoagulability. Using Laurell's method of immunoelectrophoresis for levels of alpha 2 M high levels of this are shown contrasting with progressively lowered antithrombitic action. These paradoxes no doubt arise from the fact that neonatal haemostasis is analysed using standardised techniques and reactions which were developed for adult haemostasis, from which it is certainly as different as from those of other animals. Neonatal haemostasis is perfectly balanced in normal conditions. Important changes occur however in respiratory distress where there is a drop in factor V and soluble complexes appear, bringing about rough shapes suggestive of the subclinical syndrome of defibrination. This situation will be further developed in a forthcoming article.

Blood Coagulation Factors↗
PubMed1975

Indirect sulphonation of mucopolysaccharides and glycogen in tissue sections with the use of bisulphite or dithionite after periodic acid oxidation.

Tissue mucopolysaccharides and glycogen can be indirectly sulphated after being oxidized by periodic acid and treated with sodium bisulphite or dithionite in aqueous solution. The sulphated sites are darkly stained by Roluidine Blue and realted dyes at pH less than 1.0. The background is very pale or colourless. The stained sections resist extraction with 1% hydrochloric acid for 48 hr, but can be extracted by 5% ammonium hydroxide in ethanol in 1 hr. Other oxidizing agents cannot be substituted for periodic acid.

Dithionite↗
PubMed1975

The staining of Brunner's gland and other neutral mucins by carmine, hematoxylin and orcein in alkaline solutions.

Brunner's glands and other neutral mucins may be stained red, brownish red, and violet, respectively, by carmine, hematoxylin, and orcein from appropriate alkaline solutions. Carmine and hematoxylin in concentrations of 0.2-1% are dissolved in 60-70% alcohol containing 1% potassium carbonate; orcein is used in a 0.2% alcoholic solution of sodium hydroxide. Staining times are 15 to 30 minutes. The stained sections are rinsed in 95% or absolute alcohol prior to xylene and mounting. The staining of these mucins is blocked by mild bromine oxidation. By using alcian blue 0.1% in 3% acetic acid for 5 minutes prior to the above stains, mucins may be characterized in the same preparation as acid, neutral or mixed.

Alkalies↗
PubMed1975

Histochemical factors influencing the sulfation of the mucosubstances of the Brunner's glands of the human duodenum.

The mucopolysaccharides of the human Brunner's glands were sulfated by sulfuric acid diluted with acetic anhydride or nitrobenzene (1:50) in 5 min. They were recognized by toluidine blue at a pH of less than 1 by exhibiting a red-violet or violet-red metachromasia. The sulfated radicals were removed by methanol or butanol with HCl, H2SO4, Na or NaOH, aqueous Ba(OH)2 at 60 degrees C, or by acetic anhydride, acetyl chloride, benzoyl chloride, or bromine water at 25 degrees C. The carbohydrates were altered so that sulfation was prevented by prior treatment with aqueous Ba(OH)2, or by acetic anhydride with pyridine at 60 degrees C and by acetyl chloride and by bromine water at 25 degrees C. Periodic acid Schiff staining was prevented by sulfation with a nitrobenzene: H2SO4 mixture but not by an acetic anhydride: H2SO4 combination in 1 h suggesting an additional sulfate radical at hydroxyl sites 1 or 2. Brunner's glands contain a large amount of a neutral mucopolysaccharide and can be used as a model for testing a large number of chemical and blockage reactions. In nearly all instances, Brunner's glands reacted more like pyloric glands than duodenal goblet cells.

Acetates↗