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Biomedical subjects

C Baglioni

Publications and source records attributed to C Baglioni.

At least 181 records · Page 10Linked to original sources
PubMed1972

Messenger RNA for globin in the postribosomal supernatant of rabbit reticulocytes.

10S RNA has been isolated from the postribosomal supernatant of rabbit reticulocyte lysate. This RNA is associated with protein in a ribonucleoprotein particle that sediments at approximately 20S. The 10S RNA extracted from this particle codes predominantly for alpha globin chains when it is translated by a mouse ascites cell-free extract. The product of the cell-free synthesis has been characterized by column chromatography and electrophoresis of the tryptic peptides. About 20% of the total alpha chain mRNA is present in the postribosomal supernatant, whereas beta chain mRNA is almost exclusively associated with polyribosomes.

Animals↗
PubMed1972

Translation of messenger RNA for histones from HeLa cells by a cell-free extract from mouse ascites tumor.

7-9S RNA has been isolated from polyribosomes of HeLa S-3 cells in S phase. These RNA species direct the synthesis in vitro of all five classes of human histones when they are added to a protein-synthesizing system derived from mouse ascites tumor cells. The histones synthesized in vitro have been identified by two kinds of acrylamide gel electrophoresis, and by paper electrophoresis of tryptic digests. By use of the ratio of tyrosine to tryptophan incorporation as an index of contamination, it was determined that less than 10% of the 7-9S RNA is translated into proteins other than histones.

Amino Acids↗