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Biomedical subjects

C Babinet

Publications and source records attributed to C Babinet.

At least 127 records · Page 7Linked to original sources
PubMed1980

A cell surface glycoprotein involved in the compaction of embryonal carcinoma cells and cleavage stage embryos.

Fab fragments of rabbit anti-embryonal carcinoma cells IgG dramatically perturb cell-cell interactions between embryonal carcinoma cells and between early mouse embryo blastomeres. These antibodies prevent compaction of preimplantation embryos (or trigger their decompaction) and have similar effects on embryonal carcinoma cells. They probably act through the masking of specific molecules (Fab targets) involved in the mechanisms of recognition between cells during compaction. Fab target molecules have been extracted from embryonal carcinoma cell membranes and purified using their property to inhibit the effects mediated by anti-embryonal carcinoma Fab. The solubilization of the Fab targets could be achieved using both detergent extraction and trypsin treatment of membranes. In the latter case, a glycoprotein of 84,000 daltons could be purified which has all the properties expected from the Fab target and accounts for most of the Fab-inhibiting activity of embryonal carcinoma cell membranes.

Animals↗
PubMed1980

Monoclonal antibodies against trophectoderm-specific markers during mouse blastocyst formation.

Two-dimensional gel electrophoresis has allowed the detection of proteins characteristic of inner cell mass and trophectoderm in mouse blastocyst. Certain of the proteins characterizing trophectoderm copurify with intermediate filaments from trophectoderm and a trophoblastoma cell line. A monoclonal antibody prepared against proteins of these intermediate filaments labels a filament network in trophectoderm but not in inner cell mass cells.

Animals↗
PubMed1980

Chimaeric rabbits from immunosurgically-prepared inner-cell-mass transplantation.

Rabbit inner cell masses (ICMs) have been prepared by immunosurgery from 96-h-old blastocysts. Their properties are described. It is shown that ICMs prepared this way retain their developmental potentialities. In particular if they are injected into host blastocysts, they appear to be able to participate in the development of the composite blastocysts thus obtained: upon implantation into a foster-mother the latter give rise, in a high proportion of cases, to chimaeric rabbits. Immunoglobulin allotypic markers as well as progeny test were used to assess chimaerism.

Animals↗
PubMed1978

Participation of cultured teratocarcinoma cells in mouse embryogenesis.

Mouse blastocysts were microsurgically injected with embryonal carcinoma cells from in vitro teratocarcinoma cell lines C17, C86, SIKR-OSB, and PCC3/A/I. The embryos were allowed to develop to term and the resulting offspring were analysed for chimaerism using coat colour markers and isozyme differences of the enzyme glucose phosphate isomerase. When injected into blastocysts, cell line C86 produced tumours in six of 74 animals born. The tumours were detected at birth and were poorly differentiated neuroectodermal teratocarcinomas. Cell line C17 gave 13 chimaeras in 77 mice born, five of which showed chimaerism only in normal tissues, mainly melanocytes of the coat and eye. The other eight chimaeras developed tumours. Seven of these developed in adult animals and were mainly fibrosarcomas. Cell line SIKR-OSB resulted in one normal chimaera in 44 mice born. Of 86 animals born following injection of cell line PCC3/A/1, there was one chimaera with a small tumour and three normal chimaeras. The levels of chimaerism were generally very low. The mice were test bred but with no evidence of germ line chimaerism. The karyotypes of all the cell lines were abnormal. How this and other factors such as cell cycle times might affect the incorporation of embryonal cells into the developing embryo is discussed.

Animals↗
PubMed1977

Surface antigen in early differentiation.

Addition of Fab fragments from rabbit antiserum to surface antigen F9 to 2-cell stage mouse embryos in culture does not alter cleavage; however, the addition prevents culture does not alter cleavage; however, the addition prevents the formation of compact morulae and blastocysts. A similar effect is observed when Fab fragments are added to already compact 8-cell stage or even older morulae, but disappears at the beginning of blastocoel formation. This effect is reversible: uncompact 30-cell embryos washed free of Fab become compact in a few hours, produce blastocysts, and upon reimplantation into pseudopregnant mothers can produce mice. Development is not altered by divalent anti-F9 antibodies, by Fab fragments from sera directed against other embryo surface antigens, or by succinyl concanavalin A.

Animals↗
PubMed1976

Embryonal carcinoma antigen and the T/t locus of the mouse.

The presence of the F9 antigen and of four other antigens related to the T/t locus of the mouse was investigated by immunofluorescence on preimplantation embryos. In morulae heterozygous for any of these t haplotypes, both the appropriate t antigen and the F9 antigen are expressed. The F9 antigen segregates among the progeny of crosses producing embryos homozygous for some (tw32 and tw5) but not for other haplotypes. It is concluded that (i) whatever the time of action of a t haplotype, its corresponding antigen is expressed during cleavage and (ii) the F9 antigen is specified by a gene(s) in the region of the T/t locus.

Animals↗
PubMed1973

Surface antigens common to mouse cleavage embryos and primitive teratocarcinoma cells in culture.

Syngeneic antisera have been produced in mouse strain 129/Sv-CP males against the primitive cells of teratocarcinoma. These sera react specifically with the primitive cells and are negative on various types of differentiated teratoma cells derived from the same original tumor. They are negative on all other mouse cells tested, with the exception of male germ cells and cleavage-stage embryos. Thus, teratoma cells possess cell-surface antigens in common with normal cleavage-stage embryos.

AKR murine leukemia virus↗
PubMedDate not supplied

Mutation of immunoglobulin J chi splice sites does not affect the rearrangement frequency of J chi segments.

The rabbit b9 kappa 1 locus contains 5 joining (J chi) gene segments, only two of which, J chi 1 and J chi 2, are utilized in assembly of a complete variable region gene (as shown by Akimenko, M.-A., Mariamé, B. and Rougeon, F. (1986) Proc. natl. Acad. Sci. (Wash.), 83, 5180-5183). J chi 4 and J chi 5 do not rearrange because of deficient recombination signal sequences. The J chi 3 gene segment is also not rearranged and has a non-functional splice site. In view of the proposed relationship between transcription and rearrangement of immunoglobulin genes, we sought to determine whether splicing of the germline transcript is implicated in the rearrangement process. We addressed this question by introducing mutations in the splice sites of the J chi segments of an unrearranged kappa light chain immunoglobulin transgene. Rearrangement was analysed by polymerase chain reaction on transgenic spleen DNA. We observed that mutation of the splice sites had no effect on the utilization of the J chi gene segments, demonstrating that there is no relationship between splicing and rearrangement.

Animals↗