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Biomedical subjects

C B Thomas

Publications and source records attributed to C B Thomas.

128 records · Page 8Linked to original sources
PubMedDate not supplied

Detection of antigenic variation among strains of Mycoplasma gallisepticum by enzyme-linked immunosorbent inhibition assay (ELISIA) and Western blot analysis.

Polyclonal antisera (PCA) to three Mycoplasma gallisepticum (MG) strains (F, S6, and A5969) produced in rabbits were used in enzyme-linked immunosorbent inhibition assay (ELISIA) and Western blot analysis to examine antigenic variability among these strains of MG. In ELISIA, inhibiting antigen of the same strain used for immunization always led to the greatest percentage inhibition of PCA reactivity. Western blot analysis of antigens of four MG strains demonstrated both common and restricted patterns of immune recognition among the three PCAs.

Animals↗
PubMedDate not supplied

Factors affecting HPRT mutant frequency in T-lymphocytes of smokers and nonsmokers.

The frequency of thioguanine-resistant, hypoxanthine phosphoribosyltransferase-deficient lymphocytes in the peripheral blood of human subjects was used to study the genotoxic effects of smoking. Sixty-two nonsmokers and 58 smokers, aged 19 to 45 years with average ages of 30 and 32 years, respectively, and with no other known exposures, were studied using an in vitro assay of the frequency of mutant lymphocyte clones. Analysis of variance explained 68% of the variation in the mutant frequencies. Mutant frequency was dependent upon lymphocyte cloning efficiency, length of smoking history, age, and interactions between these variables. Four nonsmokers and three smokers had high mutant frequencies that were not explained by these variables. Mutant frequencies were inversely related to lymphocyte cloning efficiencies; the effect was twice as great for smokers as for nonsmokers. The time-dependent effect of smoking dominated, with mutant frequency increasing 10%/year of smoking as compared with an independent 1%/year of age. Smoking had a greater effect on young smokers' lymphocytes. Heterogeneity of mutant frequency among both smokers and nonsmokers and its implications for use of lymphocyte mutation assays as biomarkers are discussed.

Adult↗