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Biomedical subjects

C B Loadholt

Publications and source records attributed to C B Loadholt.

52 records · Page 3Linked to original sources
PubMed1976

Evaluation of four methods for separation of lymphocytes from normal individuals and patients with cancer and tuberculosis.

An optimal technique was sought for lymphocyte recovery from normal and chronic diseased individuals. Lymphocytes were separated by four techniques: Plasmagel, Ficoll--Hypaque, a commercial semiautomatic method, and simple centrifugation using blood drawn from ten normal individuals, ten cancer patients, and ten tuberculosis patients. The lymphocyte mixture obtained after using each method was analyzed for percent recovery, amount if contamination by erythrocytes and neutrophils, and percent viability. The results show that the semiautomatic method yielded the best percent recovery of lymphocytes for normal individuals, while the simple centrifugation method contributed the highest percent recovery for cancer and tuberculosis patients. The Ficoll-Hypaque method gave the lowest erythrocyte contamination for all three types of individuals tested, while the Plasmagel method gave the lowest neutrophil contamination for all three types of individuals. The simple centrifugation method yielded all viable lymphocytes and thus gave the highest percent viability.

Cell Separation↗
PubMed1976

Ruptured appendicitis in children: continuing controversy over antibiotic combinations.

A prospective study has been made of 36 children with ruptured appendicitis. Alternate patients were managed with a standard protocol of therapy differing only in the antibiotics used. One group received a combination of penicillin/streptomycin/sulfadiazine while the other children were treated with cephalothin (Keflin) and cephalexin (Keflex). No major infections complications occurred in the P/S/S study group but there were four in the C/C patients. No serious adverse reactions due to the antibiotics occurred in either group.

Adolescent↗
PubMedDate not supplied

Prolonged ingestion of commercial DDT and PCB; effects on progesterone levels and reproduction in the mature female rat.

A report linking human polycystic ovary with increased exposure to environmental DDT (Heinrichs et al. 1971) prompted the present study comparing effects of PCB and DDT or their combination on reproduction in female rats under more realistic conditions with respect to level (75 and 150 ppm), route of administration (dietary contaminant), and period of exposure (8 and 36 weeks). Evaluation of estrous cycle length, mating frequency, number and size of litters; as well as plasma levels of DDT, PCB, progesterone (P), and 17 alpha=hydroxyprogesterone (17 alpha=OH-P), permitted comparison of short and long term reproductive changes from ingestion of two levels of DDT and/or PCB. PCB reduced plasma progesterone (p less than .01) while plasma 17alpha OH-P was unchanged by PCB or DDT. High DDT and PCB abolished reproduction. Histologically, distinct ovarian stromal changes accompanied 150 ppm of PCB, while increased numbers of more prominent follicular cysts were evident with 150 ppm of DDT. Although DDT and PCB generally reduced or abolished litter production, no treatment tested significantly altered litter size. Long term chronic ingestion of more realistic levels of technical DDT (85% p,p', 15% o,p'-DDT) in these studies did not lead to polycystic ovaries in adult rats comparable to those reported following i.v. administration of pure o,p'-DDT to immature rats. Plasma DDT levels above 800 ppb are clearly detrimental to reproduction, while levels below 500 ppb had lettle effect. Finally, we present the first evidence reported to our knowledge demonstrating that prolonged ingestion of PCB (150 ppm) markedly reduces reproduction (p less than .05) accompanied by significantly reduced progesterone in plasma (p less than .01) as well as by histologically characteristic ovarian stromal changes not seen with DDT alone.

Animals↗
PubMedDate not supplied

HLA antigens in different age groups.

HLA typing was performed for 17 antigens on cells from 189 unrelated, normal white Caucasians. No significant differences (p greater than 0.05) in distributions of different markers were found among the age groups. There does not seem to be a relation between heterozygosity at HLA-A and HLA-B loci and survival until old age.

Adult↗
PubMedDate not supplied

A modified scleroderma skin scoring method.

The extent of skin thickness in scleroderma has been reported to correlate with the degree of internal organ involvement. An objective method for the clinical evaluation of skin involvement (skin score) in scleroderma is described. In this preliminary study, the method was tested in twenty scleroderma patients. An acceptable degree of inter-observer reproducibility was noted. This method is proposed as a means of monitoring the degree of skin involvement and the overall disease activity.

Humans↗
PubMedDate not supplied

A quantitative immunofluorescence assay for IgM rheumatoid factor.

A quantitative immunofluorescence assay for IgM rheumatoid factor (RF) has been developed using the FIAX fluorometer. Two hundred and six (206) patient serum samples submitted for RF or antinuclear antibody testing were assayed by the fluorometric method and by the sheep cell slide agglutination test (Rheumaton, Wampole Laboratories) currently used in our clinical laboratory. There was an overall agreement of 95.1% between the 2 methods. The fluorometric RF assay is simple, sensitive, reproducible, and does not suffer from the subjectivity present in slide agglutination tests.

Agglutination Tests↗