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Biomedical subjects

C B Andersen

Publications and source records attributed to C B Andersen.

At least 91 records · Page 5Linked to original sources
PubMed1992

Metabolism of the aminoterminal propeptide of type III procollagen in cultures of human proximal tubular cells.

Degradation of the intact form of the aminoterminal propeptide of type III procollagen (PIIINP) has been established in the liver, whereas the col 1 domain of PIIINP is extracted by the kidneys. We used native human PIIINP and col 1 domain of PIIINP to investigate the degradation of PIIINP in cultures of human proximal tubular cells. Normal renal tissue was obtained from the healthy part of kidneys surgically removed and from biopsies from a total of 10 patients. The degradation was characterized by incubation of [125I]-PIIINP followed by gel filtration. We found that in physiological concentrations (4.4 micrograms l-1 and 11.9 micrograms l-1 intact PIIINP was almost totally degraded, but not col 1 domain. High concentrations of PIIINP (20-50 micrograms l-1) had a non-linear, non-monoexponential degradation over time, which suggests several steps. Gel filtration of [125I]-PIIINP after 1 h, 3 h, 6 h and 24 h of incubation confirmed the observation by showing the rapid formation of a high-molecular-weight fraction, followed by the slower formation of a low-molecular-weight fraction. The high-molecular-weight fraction was PIIINP immunoreactive, but not the low-molecular-weight fraction. We conclude that cultures of human proximal tubular cells degrade intact human PIIINP by the formation of high- and low-molecular-weight fractions. Earlier findings that extraction of the PIIINP col 1 domain takes place in the kidneys, cannot be explained by degradation by the proximal tubular cells.

Cells, Cultured↗
PubMed1991

Cellular inflammatory infiltrates and renal cell turnover in kidney allografts: a study using in situ hybridization and combined in situ hybridization and immunohistochemistry with a Y-chromosome-specific DNA probe and monoclonal antibodies.

The generation and targeting of inflammatory cells in acutely rejecting kidney allografts are only partly understood. In order to investigate the origin of infiltrating mononuclear cells and the renal cell turnover, percutaneous renal biopsies (45) and lost renal allografts (4) from 40 sex-mismatched transplant patients clinically suspected of developing acute rejection were analysed by in situ hybridization (ISH) and combined ISH and immunohistochemistry (IMH). A biotinylated Y-chromosome-specific DNA probe was used for ISH. Monoclonal antibodies against leukocytes (leukocyte common antigen (CD45), T lymphocytes (CD43), B lymphocytes (L26) and myeloid/histiocytic cells (mac 387] were employed using a three-stage immunoperoxidase technique followed by ISH on the same specimens. The ISH method was very sensitive when differentiating male from female cells (p less than 0.01). Posttransplant mononuclear infiltrates were shown to be of recipient origin and dominated by T lymphocytes and myeloid/histiocytic cells. Tubular and glomerular cells remained of donor origin even after 10 months. There was no evidence of revascularization by recipient endothelial cells.

Antibodies, Monoclonal↗
PubMed1991

Cyclosporine-induced renal morphologic and immunohistologic changes in patients with chronic uveitis.

Ten long-term cyclosporine-treated patients with chronic uveitis underwent percutaneous renal biopsy in order to evaluate a) abnormalities of renal morphology and b) the nature of lymphocytic infiltrates by immunohistochemistry. Pretransplant renal biopsies from eleven cadaveric donors served as controls. Eight of the ten patients had lymphocytic infiltrates consisting predominantly of T lymphocytes with a CD4+/CD8+ ratio of 1.46, which is identical to that of peripheral blood in healthy donors. Evidence of immune activation as estimated by the presence of interleukin-2 receptors, transferrin receptors or by the expression of MHC class II antigens was not demonstrated in any of the patients. The severity of morphologic alterations did not correlate with any of the clinical or paraclinical data. Percutaneous renal biopsy should be performed within 18 months of treatment to identify patients susceptible to renal side effects of cyclosporine.

Adult↗
PubMed1991

Cytomegalovirus infection in classic, endemic and epidemic Kaposi's sarcoma analyzed by in situ hybridization.

In order to evaluate the association between cytomegalovirus (CMV) infection and Kaposi's sarcoma (KS) at the cellular level, four classic KS, five endemic (African) KS and 55 epidemic KS from 12 patients with AIDS were analyzed by in situ hybridization with a biotinylated specific CMV-DNA probe. CMV-DNA was sparsely demonstrated in the sarcomas in 10 of the patients with AIDS but in none of the classic or endemic sarcomas. The distribution and localization of the CMV-infected cells did not suggest CMV as a major pathogenic stimulus for the development of KS but rather as an opportunistic infection in severely immunosuppressed patients.

Acquired Immunodeficiency Syndrome↗
PubMed1990

Detection of CMV DNA and CMV antigen in renal allograft biopsies by in situ hybridisation and immunohistochemistry.

One hundred kidney allograft biopsies from 85 patients and tissue specimens from 31 failed allografts were analysed by immunohistochemistry (IMH) with a monoclonal antibody against a cytomegalovirus (CMV) antigen and by in situ hybridisation (ISH) with a biotinylated CMV DNA probe. Six clinically and serologically CMV-infected patients with AIDS served as positive controls. Biopsies from six seronegative donor kidneys and autopsy specimens from five clinically and serologically negative patients served as negative controls. Adequate serology in 56 patients showed 50% to be actively CMV infected. No statistically significant difference was found between the frequency of acute rejection in the seropositive and the seronegative patients. Four seropositive transplanted patients contained CMV DNA or CMV antigen in the biopsies and in the grafts. Both methods gave a negative result in 15 patients with glomerulopathy. Expression of MHC class I and II antigens in 17 of the biopsies demonstrated no relation to positive serology or local demonstration of CMV. In conclusion, (1) CMV was not renotropic, (2) there was no correlation between (a) CMV and acute rejection, (b) CMV and glomerulopathy, or (c) CMV and MHC class I and II antigen expression. The study suggests that CMV does not play a major role in rejection.

Antigens, Viral↗
PubMed1990

Detection of cytomegalovirus-infected cells in autopsy material by in situ hybridization.

A non-radioactive in situ hybridization (ISH) method was elaborated to detect cytomegalovirus (CMV)-infected cells in tissue specimens processed for diagnostic routine histopathology. A biotinylated CMV-DNA probe was hybridized following a) four different enzymatic predigestions, b) progressively increasing denaturation periods, and then detected by c) streptavidin-biotin, d) a monoclonal antibody against biotin using a three-stage alkaline phosphatase anti-alkaline phosphatase (APAAP)-technique, and e) combining c + d. Autopsy specimens obtained from an infant with acquired CMV-infection, six patients with AIDS, five patients clinically and serologically without CMV-infection, and preoperative needle core biopsies from six renal allografts served as material. ISH was specific and more sensitive when compared to immunohistochemical (IMH) detection of CMV-antigens by a monoclonal antibody. ISH was concluded to be a rapid, practical, and sensitive tool in daily diagnostic histopathology.

Adrenal Glands↗
PubMed1990

Release and effect of prostaglandin E2 in cocultures of lymphocytes and human tubular cells.

Prostaglandin E2 reduced the expression of HLA-ABC as well as HLA-DR antigens in cultured human tubular cells, when cells were stimulated by gamma-interferon. The PGE2 release was low in tubular cell cultures, but highly increased by coculture of tubular cells and allogeneic lymphocytes, compared to cultures of unstimulated lymphocytes, or to cocultures with autologous lymphocytes. Cocultures between non-adherent mononuclear cells and tubular cells produced low amounts of PGE2, whereas cocultures of adherent monocytes and tubular cells produced equal as much PGE2 as cultures made with stimulated peripheral blood leucocytes. The adherent cells were dominated by macrophages. The proliferative response of lymphocytes in cocultures with tubular cells was low, and was increased by indomethacin. The results indicated, that presence of monocytes adherent to the tubular cells in cocultures, down-regulate the proliferative response of allogeneic lymphocytes to the tubular cells, which may in part attribute to an inhibitory effect of PGE2.

Cell Adhesion↗
PubMed1989

Inability to elicit vagal responses from the acid-infused esophagus of pigs.

Acid application in the esophagus and esophageal distension were undertaken in pigs. One group (n = 9) had operatively induced esophagitis. The other group (n = 7) was normal. We found no discrepancy between the groups neither in inspiratory pressure nor in expiratory PCO2 nor in plasma concentrations of pancreatic polypeptide. The results indicate inability to elicit vagal responses from the acid-infused esophagus of pigs.

Animals↗
PubMed1988

Subungual keratoacanthoma. Case report.

A case of subungual keratoacanthoma in a 64-year-old man with psoriasis is described. Important differential diagnoses are squamous cell carcinoma and epidermoid cyst. The treatment recommended is local removal. Follow up should be prolonged, as recurrence has been reported more than two years after primary operation.

Humans↗
PubMed1986

Adult rhabdomyoma of the oropharynx recurring three times within thirty-five years.

A case of recurrent rhabdomyoma in the oropharynx of a 72-year-old man is presented. Diagnosis was based on routine histology, and in the third and last recurrence it was further established by electron microscopy, immune peroxidase staining for myoglobin, actin and fibronectin, and special strains. All recurrences were histologically identical and metastases were never observed. The adult rhabdomyoma is almost exclusively located in the head/neck region, often adjacent to vital organs, complicating radical surgery. The present case indicates that recurrences can be expected often at extraordinarily long intervals.

Aged↗