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C Almici

Publications and source records attributed to C Almici.

At least 73 records · Page 4Linked to original sources
PubMedDate not supplied

Human peripheral blood mononuclear cell subfractionation using counterflow centrifugation elutriation.

BACKGROUND: Counterflow Centrifugation Elutriation (CCE) can be used to separate different amounts of cells into distinct subpopulations on the basis of different sedimentation characteristics. It was the aim of this study to evaluate CCE as a technique for fractionation of normal human peripheral blood. METHODS: Human peripheral blood mononuclear cells (PBMC) were separated into three fractions by means of CCE. RESULTS: The first two fractions, eluted at a flow rate of 21 ml/min and 25 ml/min, respectively, were composed of lymphocytes with less than 2% and 4% contaminating monocytes. The cells in these fractions were capable of responding to T-cell mitogens, such as phytohemagglutinin (PHA). The third fraction, collected at the termination of the run, was composed predominantly (greater than 85%) of large monocytes that strongly labelled leu-M3 (CD 14); these cells were unable to respond to PHA stimulation. The cell recovery after CCE was considerably high (greater than 93%), and all fractions retained high cell viability (greater than 96%) and were available for further manipulation. CONCLUSIONS: In conclusion, our data demonstrate that CCE is a technique suitable for large-scale separations that makes highly purified cell fractions available for further manipulation.

Cell Separation↗
PubMedDate not supplied

Leukemic lymphomas.

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Aged↗
PubMedDate not supplied

Granulocyte colony-stimulating factor (G-CSF) prevents dose-limiting neutropenia in lymphoma patients receiving standard dose chemotherapy.

In this study, nine patients with non-Hodgkin's lymphoma (n = 6) and Hodgkin's disease (n = 3) receiving different cytotoxic chemotherapy regimens were given granulocyte colony-stimulating factor (G-CSF) (5 micrograms/kg/day) from 48 hours after the end of chemotherapy to 48 hours before the next chemotherapy administration. The decrease in mean absolute neutrophil counts (ANC) and in mean platelet (Plt) counts was not significant when pre-therapy counts were compared with post-therapy ones (p < 0.375 and p > 0.4, respectively). The mean actual dose intensity was 92% (range 68-100%). G-CSF treatment after chemotherapy reduces neutropenia and permits administration of the full chemotherapy program. A wash-out period between G-CSF treatment and chemotherapy administration is needed to prevent the detrimental effect of chemotherapy on leukocyte and platelet recovery when repeated cycles of cytotoxic drugs and G-CSF are administered.

Adolescent↗
PubMedDate not supplied

[Craniometric findings in sickle-cell anemia].

Craniometric measurements have been taken on teleradiographs in lateral and antero-posterior projection of five patients suffering from sickle-cell anaemia. All were Sicilian. The investigations revealed a proportional dysharmony among the various segments of the face, the results of excessive development of the mandibular bone, particularly transversally. This characteristic would appear to be peculiar to patients with homo- and heterozygote haemoglobinopathy.

Adult↗