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Biomedical subjects

C Abe

Publications and source records attributed to C Abe.

At least 127 records · Page 7Linked to original sources
PubMed1981

Comparison of the effects of a multi-component vaccine and a formalin-killed cell vaccine on protection against enzootic of hemorrhagic pneumonia due to Pseudomonas aeruginosa in mink.

Effectiveness of a multi-component vaccine consisting of the common antigen (OEP) derived from Pseudomonas aeruginosa strain N 10 (serotype E) and toxoids of protease and elastase was compared with that of formalin-killed cells of strain N 10 on protection against enzootic of hemorrhagic pneumonia due to P. aeruginosa in mink. One administration of the multi-component vaccine (100 microgram each of OEP, protease toxoid and elastase toxoid) clearly prevented enzootic of hemorrhagic pneumonia due to P. aeruginosa (serotype G) in mink, while a vaccination of formalin-killed cells was much less effective in preventing an epidemic. The difference in mortality rates between two vaccines was remarkable.

Animals↗
PubMed1981

[Effect of Y-12, 141 on immune response of dd mice. Studies on anti-allergic agents, V. (author's transl)].

Mice were immunized with sheep red blood cells (SRBC) at day 0, and hemagglutinin (HA) titer of the serum, hemolytic plaque forming cells (HPFC) in the spleen cells and rosette forming cells (RFC) in both the spleen and thymus cells were assayed at day 5. The immune responses in the mice immunized with 1 X 10(8) SRBC intravenously were not affected by Y-12, 141 given orally for 5 days (0 approximately 4 days) at doses of 30 approximately 100 mg/kg, but were suppressed by cyclophosphamide (10 approximately 30 mg/kg p.o.) and D-penicillamine (100 mg/kg p.o.). The treatment of the mice with levamisole (1 approximately 30 mg/kg p.o.) resulted in the increase of RFC number in the spleen. Levamisole increased the number of RFC in the mice immunized with 5 X 10(6) approximately 10(8) SRBC. On the other hand, Y-12,141 (10 approximately 30 mg/kg p.o.) increased spleen RFC number in the mice immunized with 5 approximately 10 X 10(6) SRBC. Y-12,141 and levamisole given orally on day 0, 1 or 2 increased spleen RFC number in the mice immunized with 5 X 10(6) SRBC. D-Penicillamine given orally for 3 days (-2 approximately 0 days) also potentiated spleen rosette formation. The decreased number of RFC in the spleen and thymus by cyclophosphamide given orally in a dose of 20 mg/kg was restored by the treatment with Y-12,141 (3 approximately 30 mg/kg) and levamisole (1 approximately 30 mg/kg). In addition, these agents suppressed 19S HA titer, and elevated 7S HA titer in the cyclophosphamide-treated mice. Prednisolone given orally in a dose of 30 mg/kg also decreased the number of RFC in the spleen. This decrease was restored by the treatment with Y-12,141 and levamisole. These findings suggest that Y-12,141 has a capacity to potentiate the immune response in mice immunized with low doses of SRBC and to restore the decrease of immune response caused by immunosuppressive drugs such as cyclophosphamide and prendisolone.

Adjuvants, Immunologic↗
PubMed1981

Studies on pathogenicity and persistence of Propionibacterium acnes in mice.

The pathogenicity of Propionibacterium acnes was studied using immunodeficient and germ-free (GF) mice. P. acnes injected i.p. to immunocompetent (ddY and BALB/c-nu/+) and -deficient mice (CBA/N and BALB/c-nu/nu) decreased rapidly and only as small number were recovered from organs from day 7 on. The organisms injected similarly into GF-immunocompetent (GF-CF1) mice also decreased but when injected into GF-immunodeficient (GF-BALB/c-nu/nu) mice a small number persisted as late as week 4. When GF-immunocompetent (GF-ICR) mice were treated with cortisone and anti-mouse thymocyte serum, a small number of organisms were also recovered for 4 weeks after injection. Upon injection into irradiated GF-nu/nu mice, the number of viable P. acnes decreased relatively slowly and were recovered even at week 22. After oral administration a small number of P. acnes were readily detected from organs of GF-nu/nu mice but rarely from GF-nu/+ mice. The results indicate that P. acnes organisms have very low pathogenicity to mouse. However, small numbers persisted in organs of P. acnes-monoassociated immunodeficient mice. The mechanisms of this persistency of the nonpathogenic bacteria is discussed.

Animals↗
PubMed1980

Adjuvant polyarthritis. IV. Induction by a synthetic adjuvant: immunologic, histopathologic, and other studies.

A solution of an apparently nonimmunogenic synthetic compound, N,N-dioctadecyl-N',N'-bis(2-hydroxyethyl) propanediamine (CP-20961), suspended in mineral oil or olive oil (50 mg/ml), induced an acute, as well as a chronic, polyarthritis when single intradermal injections (0.2 ml) were made in the tail or hindpaw of Lewis rats. The polyarthritis was morphologically almost indistinguishable from classic adjuvant arthritis induced by Freund's complete adjuvant (FCA), a disease generally thought to be the result of a delayed hypersensitivity reaction to a constituent(s) of the injected tubercle bacilli. The disease induced by CP-20961 and that induced by Freund's complete adjuvant followed the same time course and almost identical pattern of development of clinical and histopathologic features. Like the classic adjuvant arthritis, CP-20961 induced arthritis is suppressed by an immunosuppressive agent (cyclophosphamide) or an antiinflammatory drug (phenylbutazone). The alkyldiamine (CP-20961) was found to be a potent adjuvant; a dispersion or a solution of the compound in mineral oil administered intraperitoneally enhanced the development of both the cell-mediated and the humoral immune responses to EL4 cells in the rat. These findings suggest that the immunogen responsible for the development of adjuvant arthritis is endogensou, e.g., a constituent of host tissue, a viral protein, or some complex of the two.

Animals↗
PubMed1979

Scleroderma after cosmetic surgery: four cases of human adjuvant disease.

In 9 cases of human adjuvant disease, 4 cases of scleroderma (3 progressive systemic sclerosis and 1 localized morphea) were observed. Seven to 19 years after injection of foreign substances into the breasts or nose for cosmetic purposes, some patients developed human adjuvant disease. In one case the foreign substances were removed by bilateral mastectomy with no discernible effects on the patient's clinical course. Histopathologic findings of the removed breasts demonstrated foreign body granulomas with calcification. The injected substance was identified as a mixture of liquid and solid paraffin. Human adjuvant disease might be caused by prolonged hypersensitization activated by the injected foreign materials which act as an adjuvant.

Adult↗
PubMed1979

Regions of the lipopolysaccharide of Pseudomonas aeruginosa essential for antitumor and interferon-inducing activities.

Resistance against ascites tumor development and interferon-inducing activity were demonstrated in lipopolysaccharide derived from the protein-lipopolysaccharide complex obtained from an autolysate of Pseudomonas aeruginosa. Lipid A obtained from the lipopolysaccharide was sufficient to induce interferon in vitro but no antitumor activity was found if lipid A or the polysaccharide derived from lipopolysaccharide was injected into the animal. Chemical modification of the polysaccharide portion or deacylation of the lipopolysaccharide also diminished antitumor activity. In contrast, interferon was induced by these incomplete lipopolysaccharides. These results indicate that both the lipid A portion and covalently linked polysaccharide are necessary for the inhibition of ascites tumor development, whereas incomplete lipid A with amide-linked fatty acids is sufficient to induce interferon in vitro.

Amino Acids↗
PubMed1979

Protection against hemorrhagic pneumonia of mink by Pseudomonas aeruginosa multicomponent vaccine.

An attempt to prevent epidemics of hemorrhagic pneumonia in mink due to Pseudomonas aeruginosa was made in the course of epidemics with injection of the multicomponent vaccine consisting of common protective antigen (OEP) of P. aeruginosa mixed with toxoids of protease and elastase of the bacillus. Enzootics of hemorrhagic pneumonia, due to P. aeruginosa serotype 8, broke out from August to October 1977 in a total of 13 sheds of 3 farms (A, B and C) which were located in the northeast area of Hokkaido. These farms were raising 7,452, 2,553 and 10,639 mink respectively. The mortality rate of the mink on farms A, B and C were 11.8%, 13.0% and 1.0% respectively. The vaccination was performed on the 3 farms 5, 8 and 21 days after the onset of the disease. Inoculation of each mink with 200 micrograms or 100 micrograms of each of the three components of the multicomponent vaccine was effective in most of the male and female groups of mink. The period required for revealing the effect of the vaccination was very short, in some cases only a few days. Administration of the vaccine 21 days after the onset of the enzootic was also effective.

Animals↗
PubMed1978

A compound possessing antitumor and interferon-inducing activities derived from the common antigen (OEP) of Pseudomonas aeruginosa.

OEP, a component consisting mainly of protein with small amounts of lipids and sugars, has been isolated from the autolysate of Pseudomonas aeruginosa and purified by physicochemical methods. It possesses remarkable biological properties, showing antitumor and interferon-inducing activities. As regards the antitumor activity of the sample, the ED50 value against ascites sarcoma-180 was 1 microgram/kg mouse/day, and its interferon-inducing activity amounted to 15 units at a concentration of 0.01 microgram/ml. Both activities increased after protease digestion, reaching about ten times those of the sample which had not undergone digestion. The protease-treated OEP contained 17% protein, 14.5% total sugars, 31% lipids, 12.5% hexosamine, 3.8% KDO, and 2.7% phosphorus. Neutral sugars consisted of 12.4% rhamnose, 2.7% mannose, 66.9% glucose, and other unidentified material. Total lipids derived from OEP consisted of 65% loosely-bound and 35% covalently-bound lipids; the former contained C14:10, C16:0, C16:1, C18:0, and C15:1 acids and the latter, beta-OH C10:0, C12:0, alpha-OH C12:0, beta-OH C12:0, C16:0, and C16:1 acids. The antitumor and interferon-inducing activities of OEP remained after the removal of loosely-bound lipids from OEP.

Amino Acids↗
PubMed1978

Effectiveness of immunization with single and multi-component vaccines prepared from a common antigen (OEP), protease and elastase toxoids of Pseudomonas aeruginosa on protection against hemorrhagic pneumonia in mink due to P. aeruginosa.

Toxoids of protease and elastase of Pseudomonas aeruginosa were successfully prepared by treatment with 8% formalin plus 0.2 m lysine and by 4% formalin respectively. The two toxoids proved sufficiently potent to elicit high antibody titers as estimated by both the enzyme-neutralizing and passive hemagglutination tests. The effectiveness of immunizing minks with a single component-vaccine consisting of the common antigen (OEP) of P. aeruginosa or the protease toxoid (PT) or the elastase toxoid (ET) and with the two (PT and ET) or the three (OEP, PT and ET) component-mixed vaccines, on hemorrhagic pneumonia in minks due to the bacteria was investigated. Female Sapphire minks, 3.5 months old, were used in two experiments performed in 1975 and 1976. Minks were immunized three times in one month with a total of 1 mg of each of the three antigens in the case of a single component vaccine and with a total of 2 or 3 mg (equal amounts of each component) in the case of the two or three component vaccines. Two or three weeks after the last immunization, challenge exposure with strain No. 5 was carried out by intranasally inoculating an inoculum containing serial dilutions of 10(3) -10(10) of live bacteria. Summarizing the results of the two experiments, in the case of controls, nonimmunized minks and minks immunized with potassium aluminum sulfate (potash alum) alone, the LD50 values were approximately 10(3) -10(4) with no significant difference between the two. In the case of OEP-vaccinated minks, the LD50 value was about 10(6) and thus clearly differed from those of the controls. In minks immunized with the three-component-vaccine, however, the LD50 value was about 10(8) -10(9), which indicated that the three-component-mixed vaccine was remarkably more effective than the single OEP vaccine component. In minks immunized with either PT or ET or both, the LD50 values were about 10(8) -10(9). The effectiveness of the vaccine made with ET or PT alone is discussed in the text. The pathological findings of the minks which died or survived are described.

Animals↗