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Biomedical subjects

C Abe

Publications and source records attributed to C Abe.

At least 91 records · Page 5Linked to original sources
PubMed1986

Effects of immunomodulators on candidacidal activity of normal peritoneal cells in BALB/c mice.

Macrophages which play an important role in host resistance against infections are considered to be a target for various immunomodulators. In order to examine the effects of selected immunomodulators on macrophage function, the effects of N-(2-mercapto-2-methylpropanoyl)-L-cysteine (SA96), levamisole (LMS) and D-penicillamine (D-Pc) on the candidacidal activity of the normal peritoneal cells (PC) were investigated. In the in vitro studies, SA96 increased the candidacidal activity of PC 4-fold, while D-Pc and LMS treatments did not increase this activity. In the ex vivo studies, PC obtained from the mice pre-treated with SA96, D-Pc and LMS showed a high candidacidal activity. Elevated candidacidal activity was not due to changes of cell types of PC populations. These results suggest that D-Pc and LMS activate the function of macrophage in vivo and SA96 activates the function both in vitro and in vivo.

Animals↗
PubMed1986

Monoclonal antibodies against the nonhemagglutinating fimbrial antigen 1C (pseudotype 1) of Escherichia coli.

Hybridoma-derived monoclonal antibodies were produced with fimbrial preparations from Escherichia coli 20025 (04:K12:H-) with fimbrial (F) antigens 1C, 13, one related to 12, and one preliminarily termed y and from E. coli 2980 (018ac:K5:H-) with F antigens 1C and 8. Two clones of subclonal hybrid cells were studied which produced monoclonal antibodies (mc-20025-F2b, immunoglobulin G2b [IgG2b]; mc-2980-F2, IgG1) that were reactive with E. coli 20025, 2980, and a number of additional strains which exhibited the F1C antigen. Results of enzyme-linked immunosorbent assay and Western blot analysis indicated that the antibodies had F1C specificity, and competitive enzyme-linked immunosorbent assay with 125I-labeled antibodies showed that they recognized different epitopes on the fimbrial subunit. Neither of the antibodies agglutinated F1C-fimbriated E. coli but bound to the bacteria. There was no binding to E. coli without F1C fimbriae.

Adhesiveness↗
PubMed1986

Quantitative analysis of global and regional cardiac performance in normal subjects and patients with coronary artery disease by rest/exercise radionuclide blood-pool study.

This study was undertaken to assess regional and diastolic left ventricular (LV) function in relation to LV asynchronous contraction during exercise (Ex). Rest and serial Ex (25w-75w) gated blood-pool study (Tc-99m-RBC) of 6 normal subjects (N), 12 patients with stable angina pectoris (AP) and 8 with LV aneurysm (LVan) were evaluated in regional LV ejection fraction (EF) and Fourier analysis using higher-order harmonics. In the patients with Ex and regional EF showed various responses to Ex. Global LVEF in the patients with AP indicated almost no change during Ex and regional EF declined apparently in anteroseptal segment, corresponding to stenotic left anterior descending artery. Diastolic indices of regional and global cardiac performance, PFR (peak filling rate), TPF (time to PFR) and standard deviation (SD) of TPF histogram, were obtained by Fourier analysis using two-order harmonics. Patients with coronary artery disease (CAD) showed significantly lower PFR, longer TPF and higher SD of TPF than normal group, suggesting asynchronous relaxation. We conclude that regional EF response and diastolic indices of cardiac performance to exercise provide useful and sensitive information detecting the severity of CAD.

Adult↗
PubMed1986

[Restorative effect of traxanox on the suppression of antibody production in BALB/c mice].

The number of spleen-rosette forming cells (RFC) and thymus-RFC was assayed on day 4 after immunization with 5 X 10(8) sheep red blood cells (SRBC) in BALB/c mice. The number was decreased significantly by oral administration of cyclophosphamide (20 mg/kg) on days--1 and 0. The decrease in spleen-RFC and thymus-RFC by the pretreatment with cyclophosphamide was significantly restored or had a tendency to be restored by traxanox at doses of 3 and 30 mg/kg (p.o.). In addition, traxanox (30 mg/kg, p.o.) protected the reduced number of RFC in mice treated with cyclophosphamide (3 mg/kg, p.o.). Traxanox (30 mg/kg, p.o.) restored the decreased number of spleen-RFC and thymus-RFC in mice pretreated with dexamethasone (0.1 mg/kg, p.o.), and protected the decrease in the RFC in mice treated with prednisolone (10 mg/kg, p.o.). Also, phagocytosis of SRBC by the spleen adherent cells obtained from mice treated with dexamethasone (0.1 mg/kg, p.o.) was inhibited markedly in comparison with that by the spleen adherent cells of untreated mice. The transfer of the spleen adherent cells treated with dexamethasone into syngeneic recipient mice had no effect on the number of spleen hemolytic plaque forming cells (HPFC). On the other hand, the inhibition of phagocytosis of SRBC by spleen adherent cells of mice pretreated with dexamethasone was restored by the addition of traxanox (10 and 30 microM). The transfer of the spleen adherent cells treated with traxanox resulted in an increase in HPFC number. Traxanox (30 mg/kg, p.o.) protected the decrease in spleen-RFC and thymus-RFC in mice treated with indomethacin 1 mg/kg, p.o.) or acetylsalicylic acid (300 mg/kg, p.o.). Traxanox (3-30 mg/kg, p.o.) restored the decreased number of HPFC, spleen-RFC and thymus-RFC in mice pretreated with carrageenan (0.03 mg). The suppression of phagocytosis of SRBC by spleen adherent cells was restored by the treatment with traxanox (3 and 30 microM). The transfer of the spleen adherent cells treated with traxanox also resulted in an increase in HPFC count. These results strongly suggest that traxanox restores the artificially suppressed immune responses via macrophages.

Adjuvants, Immunologic↗
PubMed1985

Are cat lingual muscles dually innervated by the hypoglossal nerve and facial nerve?

The origins of the somatic motor nerve innervating the cat lingual muscles were studied using the horseradish peroxidase (HRP) method. Following HRP injection into the lingual muscles, labeled neurons were found not only in the hypoglossal nucleus but also in the facial nucleus, ipsilateral to the injection side. HRP-labeled neurons in the facial nucleus were principally observed in the ventromedial and ventrolateral divisions of the nucleus. This study suggests that cat lingual muscles are innervated by both hypoglossal motoneurons and some of the motoneurons in the ventromedial and ventrolateral divisions of the facial nucleus.

Animals↗
PubMed1985

[Effect of traxanox sodium on inflammatory response].

Traxanox was inactive against classic acute and subacute inflammation models such as carrageenin paw edema, UV erythema, 6-hr Evans blue-carrageenin (E-C) pleurisy and cotton pellet granuloma formation, and it failed to inhibit the production of prostaglandin E2 and a slow reacting substance from rat peritoneal leucocytes which phagocytize killed bacteria in vitro. On the other hand, traxanox inhibited the anaphylactoid reaction and decreased the pleural fluid in 24-hr E-C pleurisy. Traxanox (100 mg/kg, p.o.) also showed a tendency to suppress dextran edema and cotton pellet granuloma formation in adjuvant arthritis (AA) in rats. In experimental models of delayed type hypersensitivity (DTH), traxanox (100 mg/kg, p.o.) inhibited the accumulation of the exudate and the leucocyte migration in B. pertussis-induced pleurisy in rats. Traxanox (50 mg/kg) did not show any effect on AA in Lewis rats when administered orally for 21 days after the adjuvant inoculation, but the combined administration of traxanox with hydrocortisone (10 mg/kg, p.o.) or indomethacin (0.25 mg/kg, p.o.) resulted in a synergistic inhibition of AA. When the administration of traxanox was started 21 days before the adjuvant inoculation, it inhibited AA in a dose-dependent manner (50-100 mg/kg, p.o.). On the other hand, traxanox (100 mg/kg, p.o.) enhanced the concanavalin A-induced DTH-like skin reaction in guinea pigs. These results indicate that the mode of action of traxanox on inflammatory responses resembles that of D-penicillamine or levamisole, so that it may prove to be clinically effective in treating rheumatoid arthritis.

Anaphylaxis↗