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Biomedical subjects

C A Stahl

Publications and source records attributed to C A Stahl.

5 recordsLinked to original sources

Creatine monohydrate supplemented in swine finishing diets and fresh pork quality: II. Commercial applications.

The objectives of this study were to determine the value of supplementing creatine monohydrate (CMH) in a complete swine finishing ration and determining its effects on meat quality. Crossbred barrows (n = 59) were allotted five pens per treatment with three pigs per pen. Dietary treatments, including 20 g CMH x pig(-1) x d(-1) fed for 5, 10, or 15 d before slaughter, were compared to control pigs that received no CMH. The basal diet was a corn-soybean meal finishing diet. At 123.5 kg, pigs were delivered to a commercial packing plant (80 km) and slaughtered according to industry practices. After a 24-h chill at 4 degrees C, right-side loins were collected from the fabrication line and vacuum-packaged for delivery to the University of Missouri Meat Lab. Hams were scanned for lean content by a primal cut electromagnetic scanner. After scanning, ham pH and light reflectance (L*, a*, b*) were obtained on the gluteus medius muscle. Loin pH and light reflectance were obtained at the 10/11th-rib juncture. The posterior section of the boneless loin was weighed, vacuum-packaged, and stored for 7 d at 1 degree C. After aging, light reflectance, weights, and Warner/Bratzler shear force measurements were taken. A linear trend (P = 0.071) was observed for ham 24-h L* values, and a cubic trend was observed for ultimate loin pH (P = 0.102). Hunter L* values of the longissimus possessed a negative linear contrast (P = 0.009) after aging, revealing that the loins of those animals treated for 10 and 15 d exhibit higher L* values. A cubic trend (P = 0.057) was shown for percentage of moisture lost as purge; 5- and 10-d treatments were intermediate to control and 15-d treatments. Warner/Bratzler shear force measurements for chops aged 7 d increased in a linear fashion (P = 0.024). This data suggests that 5-d supplementation of CMH before slaughter improves several pork quality attributes. However, it seems that supplementing CMH in swine diets for 10 or 15 d could reduce the quality of fresh pork.

Aging↗

A new method for data presentation in incremental cardiorespiratory exercise testing.

In incremental cardiopulmonary exercise testing, the averaging of data is usually performed to provide group mean data for statistical purposes. They are usually presented as averaged maximum values, or as averaged data at different exercise levels. However, during incremental exercise testing the change in metabolic status may vary between subjects, thus averaging data may not classify the metabolic status accurately. We present an averaging method using a segmented ordinal scale based on individual maximal work performance and the anaerobic threshold (AT). Individual exercise data are grouped into ten classes ranging from unloaded exercise to maximal exercise. The classes are defined in relation to the AT, resulting in an ordinal scale of four classes for exercise data below the AT, one class at the AT and five classes beyond the AT. Resting and unloaded pedalling are treated as separate classes. For evaluation, this method of classification is compared to one based on an absolute scale of oxygen uptake (Cabs) and to another based on a relative scale in 10% steps of maximal oxygen uptake (Crel). Ten healthy male subjects (mean age 23.3 years) performed a ramp cycle ergometer test. When using the Cabs classification method for mean data averaging, mean values for performance at high-intensity exercise were calculated using data from only two of the ten subjects because of variations in individual work capacity. In addition, the AT data were distributed across four classes, thus anaerobic and aerobic exercise data were mixed. Using the Crel classification method enabled data for all ten subjects to be included in the calculation of every data point, but the AT values were still distributed across three classes, resulting in the mixing of anaerobic and aerobic exercise data. However, using the segmented ordinal scale method of classification enabled data from all ten subjects to be included in the calculation of all data points, and it permitted the grouping of the AT values into one class. Thus, this latter method more accurately represents the data of the whole group under study and it allows the metabolic status of the subjects to be taken into consideration.

Adult↗

The use of prostate specific antigen and prostate specific antigen density in the diagnosis of prostate cancer in a community based urology practice.

Since 1989 we have used serum prostate specific antigen (PSA) levels as an indication for ultrasound guided systematic biopsies of the prostate. Realizing that the PSA level in part reflects prostatic glandular epithelial volume, we reviewed the accumulated data on our last 2,340 biopsies to determine if the quotient of PSA and prostatic volume, prostate specific antigen density, provided any further diagnostic information. There were evaluable data for 2,020 patients. Prostate specific antigen density levels are shown to have a strong correlation with the diagnosis of prostate cancer and provide a more reliable indication for ultrasound guided biopsy of the prostate than PSA alone.

Adult↗

Separation of two responses to auxin by means of cytokinin inhibition.

A continuous growth apparatus was used to measure the effect of cytokinin on auxin-induced elongation. The soybean hypocotyl segments elicited a bi-phasic response to auxin that appeared to be two overlapping responses. The first response, which began 12 min after auxin addition, was not inhibited by cytokinin, even after long preincubation in cytokinin, but the second response to auxin, which began about 30 min after auxin addition, was completely inhibited by cytokinin. Such overlapping reactions are shown, depending on the amount of overlap, to yield a variety of summation reactions, many of which resemble rate-time curves that have been previously reported. We have shown that the transient first phase of auxin-induced elongation is very similar to acid-activated growth, while the second phase is long lasting and very likely identical to the long-term response to auxin, as extensively studied in Avena, soybean, and other elongating cells.

Cell Division↗