Behcet's syndrome--a distressing, intriguing malady.
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Biomedical subjects
Publications and source records attributed to C A Price.
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Less than 10% of the acid p-nitrophenylphosphatase activity of plant extracts was sedimentable under a variety of conditions including zonal centrifugation. The sedimentable fraction did not show the latency properties characteristic of animal lysosomes. We conclude that, at least operationally, plant acid phosphatase is a soluble enzyme and that it is not suitable as a lysosomal marker. The plant tissues employed were pea cotyledons, cauliflower florets, turnip storage organs, onion embryos, and leaves of red oak and soybean.
By means of a medium containing dextran, nuclei were isolated in high yield from cells of Gymnodinium nelsoni, a marine dinoflagellate. Most of the DNA, but less than onetenth of the RNA, of the original cells was recovered in the purified nuclei. The nuclei appeared substantially intact as observed by light or electron microscopy. The isolated nuclei were capable of incorporating tritiated uridine triphosphate into material insoluble in cold acid. The general procedure was found to be applicable also to two species of diatoms.
As reported earlier by Grimm & Allen, the addition of zinc to the sporidia of the smut fungus, Ustilago sphaerogena, evokes the formation of large amounts of cytochrome c. This occurs under conditions where the rates of increase of dry weight, RNA, and DNA remain unaffected. Actinomycin D added with zinc specifically abolishes the formation of cytochrome c. The system behaves as if cytochrome c were formed de novo.
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