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Biomedical subjects

C A Morris

Publications and source records attributed to C A Morris.

At least 109 records · Page 6Linked to original sources
PubMed1985

Self-concept as altered by the diagnosis of cancer.

From a review of the literature, it was cited that the severity of the treatment regimens for cancer results in altered self-concept with specific behaviors and emotions occurring after the diagnosis. The purpose of this study was to validate the behavioral and emotional defining characteristics of the psychological construct self-concept as it is altered by the diagnosis of cancer. Defining characteristics were derived from concept analysis and confirmed by a specified patient population.

Adult↗
PubMed1982

The control of liver regeneration after partial hepatectomy in the rat.

1. Normal rat liver slices were incubated for 24 h in culture medium to which had been added freeze-dried plasma, prepared from the blood of rats which had undergone partial hepatectomy (60-70% of liver removed) 10 min, 4 or 24 h previously. No changes in the dry weight of the slices were observed. 2. Similarly, there were no changes in the dry weight or protein content of incubated liver slices when freeze-dried plasma prepared from the blood of rats unilaterally nephrectomized 10 min, 4 or 24 h previously was added to the culture medium. 3. Slices from the remaining liver stump, taken 4 h after partial hepatectomy (60-70% of liver removed) and incubated for 4 h in culture medium, did not release a factor capable of increasing the dry weight of normal liver slices incubated in the same medium. 4. After partial hepatectomy (60-70% of liver removed), there was no increase of guanosine 3':5'-cyclic monophosphate in the remaining part of the liver. 5. No measurable amount of esterase activity was found in normal liver or in the remaining liver stump after partial hepatectomy (60-70% of liver removed). 6. It is suggested that the stimulatory mechanism for liver regeneration is of a different nature from that involved in compensatory renal hypertrophy.

Animals↗
PubMed1982

Problems in the disinfection of class 1 microbiology safety cabinets.

Microbiology safety cabinet disinfection procedures using formaldehyde have been tested. Tubercle bacilli were killed by concentrations of formaldehyde obtained by heating commercial formalin irrespective of whether the bacilli were in the cabinet free space or above the prefilters. However, Bacillus stearothermophilus spore papers for for the testing of low temperature steam/formaldehyde sterilisers were almost never sterilised and a strain of Staphylococcus epidermidis (NCTC 7944) showed a resistance intermediate between the B stearothermophilus spores and the tubercle bacilli. Tests using a vaccine strain of poliovirus type 3 indicated a considerable degree of resistance of the virus to the action of formaldehyde. No such resistance was demonstrated by vaccinia virus or echovirus 14. Chemical and biological evidence is presented which indicates that filter paper discs are an unsuitable carrier material for a challenge organism in testing the efficiency of any formaldehyde sterilising process. Recommendations are made towards developing a satisfactory test procedure.

Disinfection↗
PubMed1981

Role of a renal arginylesteropeptidase in the production of a renotrophic factor in unilaterally nephrectomized rats.

1. Renal cortical slices incubated for 4 hr in culture medium in the presence of either dibutyrylguanosine-3': 5'-cyclic monophosphate (dibutyryl cyclic GMP, 10(-6)m) or freeze-dried normal rat plasma (35 mug mg(-1) wet wt. tissue) did not show any change in dry weight or protein content. However, addition of freeze-dried normal plasma together with dibutyryl cyclic GMP led to an increase in both parameters.2. Unilateral nephrectomy produced a marked increase in the level of arginylesteropeptidase in the renal cortex of the remaining kidney. A similar increase was observed in renal cortical slices incubated with dibutyryl cyclic GMP in vitro.3. The renal cortical esteropeptidase was inhibited by phenylmethylsulphonyl fluoride (PMSF, 2 mm). The inhibitor did not, however, prevent the normal increase of dry weight and protein content of renal cortical slices incubated with plasma from unilaterally nephrectomized rats. Similarly, control normal plasma incubated with cortical slices from a kidney removed 10 min after unilateral nephrectomy became endowed with renotrophic activity (Dicker & Morris, 1980b) but this activation was abolished by PMSF.4. Control plasma treated with a purified arginylesteropeptidase and incubated for 4 hr with renal cortical slices produced a hypertrophy of the slices similar to that evoked by plasma from a unilaterally nephrectomized rat.5. Since following unilateral nephrectomy there is a rapid increase in the level of cyclic GMP in the renal cortical tissue of the remaining kidney (Dicker & Greenbaum, 1977) it is suggested that this increase leads to the induction of a specific arginylesteropeptidase. The possibility that the enzyme then cleaves a renotrophic precursor normally present in the plasma, so converting it to an active form, is discussed.

Aminopeptidases↗
PubMed1981

Trimethoprim and co-trimoxazole in the treatment of acute urinary tract infections: patient compliance and efficacy.

Patient compliance and drug efficacy and side-effects were compared in two groups of patients with symptoms of acute lower urinary tract infections. One group was treated with trimethoprim, one tablet (300 mg) once a day, and the other with co-trimoxazole, two tablets (160 mg trimethoprim, 800 mg sulphamethoxazole) twice a day; both treatments were prescribed for seven days. Patient compliance was significantly greater with trimethoprim: corrected percentage compliance rates were 97.5 per cent for trimethoprim and 79.1 per cent for co-trimoxazole (p<0.05). Trimethoprim and co-trimoxazole were of equivalent effectiveness in the control of symptoms. Side-effects were more frequent with co-trimoxazole, but the difference was not significant.

Acute Disease↗
PubMed1980

Presence of renotrophic factor in plasma of unilaterally nephrectomized rats.

1. Renal cortical slices cut in two halves were incubated separately in culture medium. One half slice was used as control while the other half was treated experimentally. After incubation, their dry weights and/or their protein content were estimated. 2. Addition to half cortical slices of 30 micrograms mg-1 of freeze-dried plasma from rats killed 24 hr after unilateral nephrectomy resulted in an increase of dry weight or protein content of the order of some 10% after 4-8 hr incubation. 3. The effect of freeze-dried plasma from rats killed 24 hr after unilateral nephrectomy was specific to the renal cortex. When added to half slices from either the renal medulla or the liver there were no changes in their dry weight. 4. Addition of 30 micrograms mg-1 of freeze-dried plasma from rats killed 24 hr after bilateral nephrectomy had no significant effect on the dry weight of half cortical slices incubated for various periods of time, from 4 to 48 hr. 5. The renotrophic activity of plasma from unilaterally nephrectomized rats, killed 24 hr after the operation, appears to be metabolized or destroyed by incubation with cortical slices in 4 hr. 6. Freeze-dried plasma from normal unilaterally nephrectomized rats produced an increase dry weight when incubated for 4 hr with half cortical slices from early (3 days) hypophysectomized rats. When incubated with half cortical slices from late (23 days) hypophysectomized rats, the increase in dry weight was about 2 times as great as that observed with cortical slices from normal animals. 7. The possible reason for the production of renotrophic activity in plasma of unilaterally nephrectomized rats, and for its absence in plasma of anephric animals, is discussed.

Animals↗
PubMed1980

Origin of the humoral factor responsible for compensatory renal hypertrophy.

1. Rats were unilaterally nephrectomized and the remaining kidney was removed 10 min after the operation.2. Cortical slices from both kidneys were incubated in culture medium in the presence of freeze-dried plasma from a control animal, for 4 hr. When renal cortical slices from a normal animal were now added to and incubated in the medium in which cortical slices from the remaining kidney (i.e. the kidney removed 10 min after unilateral nephrectomy) had been incubated, they showed an increase of protein content and dry weight. This increase did not occur when slices from normal kidneys were incubated in culture medium in which the first kidney had been incubated.3. The increase of protein content and dry weight of cortical slices from normal kidneys did not occur when incubated in culture medium in which cortical slices from the remaining kidney had been incubated when freeze-dried plasma from normal rats had been substituted by freeze-dried plasma from anephric rats.4. Since normal plasma which does not normally promote protein accretion in normal cortical slices in vitro can do so after it has been incubated with cortical slices from a kidney removed 10 min after unilateral nephrectomy, it is suggested that normal plasma contains a precursor of renal origin which is ;activated' when in the presence of a remaining kidney removed shortly after unilateral nephrectomy.

Animals↗
PubMed1978

Sterilisation in the laboratory autoclave using direct air displacement by steam.

A device using a steam injection funnel is described by means of which air can be driven quickly and surely from an autoclave load. It is simple and inexpensive, necessitates no changes in the working routine of a microbiology laboratory, and does not interfere with the operation of the autoclave in its normal mode.

Laboratories↗
PubMed1978

Quantitative recovery of spores from thermophilic spore papers.

A homogeniser (Stomacher 400) which does not damage bacteria has been used to produce an homogenate of spore papers, from which it was possible to make quantitative assessments of viable spores recoverable from such papers before and after exposure to sterilising procedures.

Agar↗