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Biomedical subjects

C A Miller

Publications and source records attributed to C A Miller.

At least 271 records · Page 15Linked to original sources
PubMed1972

Neuroblastoma: synchronization of neurite outgrowth in cultures grown on collagen.

Neurite outgrowth in cultured neuroblastoma cells is inhibited in cells grown on a collagen substratum as compared to those grown on glass. Rapid, synchronized initiation of neurite outgrowth occurs after hydrolysis of the underlying collagen with collagenase. Axonated cultures exhibit an increased RNA and protein content as compared to cultures grown on collagen.

Animals↗
PubMed1971

Rapid detection and quantitative estimation of type A botulinum toxin by electroimmunodiffusion.

An experimental system is described for the detection and quantitative estimation of type A botulinum toxin by electroimmunodiffusion. The method is shown to be rapid, specific, and quantitative. As little as 14 mouse LD(50) per 0.1 ml of type A toxin was detected within 2 hr. When applied to experimentally contaminated foods such as canned tuna, pumpkin, spinach, green beans, and sausage, the technique detected botulinum toxin rapidly and identified it as to type and quantity. A specific rabbit type A antitoxin was produced for this in vitro system since the equine antitoxin (Center for Disease Control) tested in this experiment was found to be unsuitable.

Animals↗
PubMed1970

Non-chromosomal antibiotic resistance in bacteria. 3. Isolation of the discrete transfer unit of the R-factor R1.

A covalently-closed circular DNA species, banding at a buoyant density of rho = 1.709 g/cm(3) in CsCl, has been identified in antibiotic-sensitive colonies of E. coli strain AB2463 (rec A(-)) after mating with a Proteus mirabilis strain that carries the R-factor, R1. This plasmid, which represents a stable segregant of R1 that has lost all of the drug resistance determinants present on the parent R-factor but which has retained its ability to be transferred by conjugation, fulfills the functional definition of the R-factor transfer unit (RTF).

Anti-Bacterial Agents↗
PubMedDate not supplied

The acute histopathology of MPTP in the mouse CNS.

We found that 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) rapidly induced cytopathological changes in the brain, involving some neurons selectively, as well as astrocytes and blood vessels. Dopaminergic neurons in the midbrain, as identified by immunostaining for tyrosine hydroxylase, were damaged as early as 2.5 hr after MPTP administration. Ultrastructurally, there was disruption of the endoplasmic reticulum and cytoplasmic condensation and vacuolation of the tyrosine hydroxylase reactive neurons in the substantia nigra as well as their axon terminals in the striatum. Perivascular edema was associated with vacuolation and swelling of astrocytic cytoplasm and rupture of perivascular foot processes. There was also capillary and arteriolar endothelial damage. Surprisingly, there was no clear correlation of MPTP-induced pathology with mitochondrial damage in any cell type. Biochemically, dopamine was depleted in the substantia nigra and the striatum within a few hours following MPTP administration. However, in the substantia nigra, homovanillic acid (HVA), one of the metabolites of dopamine, showed relatively less depletion than did dopamine by MPTP. These results may indicate that the turnover of dopamine was stimulated in the brain as a homeostatic mechanism.

Animals↗