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Biomedical subjects

C A Gray

Publications and source records attributed to C A Gray.

32 records · Page 2Linked to original sources
PubMed1999

Uterine differentiation as a foundation for subsequent fertility.

Uterine differentiation in cattle and sheep begins prenatally, but is completed postnatally. Mechanisms regulating this process are not well defined. However, studies of urogenital tract development in murine systems, particularly those involving tissue recombination and targeted gene mutation, indicate that the ideal uterine organizational programme evolves epigenetically through dynamic cell-cell and cell-matrix interactions that define the microenvironmental context within which gene expression occurs and may ensure adult tissue stability. In the cow and ewe, transient postnatal exposure of the developing uterus to steroids can produce immutable changes in adult uterine tissues that may alter the embryotrophic potential of the uterine environment. Thus, success of steroid-sensitive postnatal events supporting uterine growth and development can dictate the functional potential of the adult uterus. Studies to determine effects of specific steroidal agents on patterns of uterine development during defined neonatal periods, as well as the functional consequences of targeted neonatal steroid exposure in the adult uterus, should enable identification of critical developmental mechanisms and determinants of uterine integrity and function. Extreme adult uterine phenotypes (lesion models) created in cattle and sheep by strategic postnatal steroid exposure hold promise as powerful tools for the study of factors affecting uterine function and the rapid identification of novel uterine genes.

Animals↗
PubMed1996

Nippostrongylus brasiliensis: cytokine responses and nematode expulsion in normal and IL-4-deficient mice.

Infection with the nematode, Nippostrongylus brasiliensis, results in a Th2-dominated immune response. We describe the dynamics of this response in both local and systemic environments. Th2-type responses were evident first in the mesenteric lymph node, with parasite antigen-specific proliferation and IL-4/IL-5 release peaking at Days 7-9 postinfection, shortly before expulsion of the adult worms from the gut. IFN-gamma responses were not observed in the mesenteric lymph node. Responses in the spleen generally followed those in the mesenteric lymph nodes by 2-3 days and showed a greater degree of Th1-type cytokine production. N. brasiliensis was shown to be a powerful inducer of IL-4 responses with as few as six infective N. brasiliensis larvae eliciting IL-4 production in the mesenteric lymph node, but only high doses of larvae (600) elicited IL-4 secretion in the spleen. Similar levels of IL-4 production by lymph node cells stimulated with Con A or parasite antigen postinfection indicated the extent of polyclonal Th2 stimulation by this parasite. Infection of IL-4-deficient mice showed that despite the absence of IL-4-dependent Th2 responses, these mice were able to curtail egg production and expel adult N. brasiliensis in a time frame similar to that of fully immunocompetent animals. These results emphasize the magnitude of the Th2 response to N. brasiliensis and also show that IL-4 is not a prerequisite for the development of immunity to N. brasiliensis.

Animals↗
PubMed1991

Opsonic effect of C-reactive protein on phagocytosis and intracellular killing of virulent and attenuated strains of Candida albicans by human neutrophils.

In the presence of autologous complement, maximal phagocytosis of Candida albicans blastospores of both a virulent and attenuated strain by human neutrophils in a monolayer assay was achieved after 30 min. The proportion of phagocytes containing intracellular blastospores was 33-36% with an average of 1.5 blastospores per phagocyte. In contrast to the attenuated strain of C. albicans, the virulent strain resisted opsonization by C-reactive protein (CRP) and of those blastospores ingested, only 8% were killed. These findings support the concept that CRP may play a protective role in candidosis independent of complement. The fate of strains of different virulence may be a result of differences in CRP receptors or killing mechanisms.

C-Reactive Protein↗
PubMed1989

Effect of amorolfine (Ro 14-4767/002) on in vitro phagocytosis and intracellular killing of Candida albicans by human neutrophils.

The effect of amorolfine (Ro 14-4767/002) on phagocytosis and intracellular killing of Candida albicans blastospores was determined in human neutrophil monolayer assays. At 0.2, 2 and 5 micrograms/ml amorolfine did not have any significant inhibitory or enhancing effect on phagocytosis whether following simultaneous addition of blastospores and drug to the neutrophils, prior treatment of neutrophils for 2 h before addition of blastospores or prior treatment of blastospores for 2 h. Simultaneous addition of amorolfine resulted in a significant increase in killing at all concentrations. This increase was not significantly enhanced by either preincubation of neutrophils or blastospores for 2 h with the drug.

Antifungal Agents↗
PubMed1982

Prediction of IQ and language skill from perinatal status, child performance, family characteristics, and mother-infant interaction.

193 basically healthy working-class and middle-class mothers and their infants participated in a 4-year longitudinal study which focused on the relative potency of several clusters of variables for predictions of intellectual and language outcome during the preschool years. The major results were: (1) Measures of perinatal or infant physical status were extremely weak predictors of 4-year IQ or language. (2) Assessments of child performance were poor predictors prior to 24 months, but excellent predictors from 24 months on. (3) Assessments of mother-infant interaction and general environmental quality were among the best predictors at each age tested, and were as good as measures of child performance at 24 and 36 months in predicting IQ and language. (4) Measures of the family ecology (level of stress, social support, maternal education) and parent perception of the child, especially when assessed at birth, were strongly related to child IQ and language within a low-education subsample, but not among mothers with more than high school education. Patterns of prediction were similar for 48-month IQ and 36-month receptive language; predictions were notably weaker for 36-month expressive language.

Child Development↗
PubMed1981

Teacher versus parent reports of preschoolers' social competence.

The social competence of children aged three through five was assessed using the Preschool Behavior Questionnaire. Two questions were addressed: First, were the responses of mothers and preschool teachers comparable for any particular child? Second, did the factor structure of the preschool teacher responses replicate those reported by the authors of the instrument? The results showed that the mothers' response patterns, as reflected in the factor structure, proved to differ from those of the preschool teachers. However, the teachers in the Seattle sample did replicate the previously reported factor structures of preschool teachers elsewhere. In addition, it was found that the suggested scoring criteria when applied to the study population produced an unacceptably high number of children in the "deviant" classification. The results were discussed in terms of the implications for using this instrument in general screening programs.

Journal Article↗
PubMed1978

Pituitary function in Down's syndrome.

Pituitary function studies have been carried out in eight male and eight female patients with Down's syndrome who were suspected of having hypothyroidism secondary to pituitary deficiency. In all cases, the response of Human Growth Hormone to hypoglycaemia was adequate and the response of Lutenising Hormone and Follicle Stimulating Hormone to the injection of Lutenising Hormone and Follicle Stimulating Hormone was not signfiicantly greater than that in age and sex-matched institutionalised controls. Therefore there is no evidence that the relative hypothyroidism in these patients is due to pituitary hypofunction, nor do the results provide evidence of pituitary deficiency in Down's syndrome generally.

Adult↗
PubMed1968

Professional offices.

Explore the source record for details and available documents.

Health Facility Planning↗
PubMedDate not supplied

Nurse-midwives speak out on the ACNM. Results of the 1987-88 Needs Assessment Survey, Part 1.

This article presents the purposes and methods of the first Needs Assessment Survey of all nurse-midwives who have been certified by the ACNM. A detailed summary of the general results of the survey is provided, and includes the perceptions of respondents about the ACNM organizational structure, goals, leadership, and services. Analyses of differences in response based on such variables as membership status, minority status, years since certification, level of educational preparation, work setting, and region of residence are also presented. The article concludes with a discussion of the implications for the nurse-midwifery profession. Part 2 will appear in JNM 34:2.

Certification↗