Search PubMed⌕ Search

Biomedical subjects

C A Collins

Publications and source records attributed to C A Collins.

42 records · Page 3Linked to original sources
PubMedDate not supplied

Isolated flagellar outer arm dynein translocates brain microtubules in vitro.

The inner and outer arms of the flagellar axoneme generate the forces needed for flagellar movement; these arms contain ATPases called dyneins. To date, there has been no method for studying the mechanochemical transducing activity of isolated dyneins. Recently, it was found that the brain microtubule-associated protein (MAP) 1C is a microtubule-activated ATPase with the structural and force-producing properties of dynein. MAP 1C translocates microtubules in an in vitro gliding assay, suggesting that such an assay could also be used with axonemal dyneins. Here, we demonstrate that outer-arm dynein isolated from sea urchin (Strongylocentrotus purpuratus) sperm and adsorbed to a glass coverslip can translocate calf-brain microtubules along the surface of the coverslip. Our results conclusively demonstrate that outer-arm dynein by itself is capable of generating shearing forces. The ability to examine the force-generating properties of flagellar dynein in vitro should greatly facilitate studies of the mechanism of action of this important mechanochemical transducer.

Adenosine Triphosphatases↗
PubMedDate not supplied

Ascites.

Explore the source record for details and available documents.

Ascites↗