Biomedical subjects

Bo Zhang

Publications and source records attributed to Bo Zhang.

257 records · Page 15Linked to original sources

PubMed2002

[Preparation of PSAS and its application in HAB prevention].

In order to obtain optimum PSAS (Polysilicate-aluminium sulfate) preparation conditions for removing HAB organisms, the 3-factor and 3-level orthogonal test was devised. Concentration of SiO2, Al3+/SiO2 molar ratio and pH were chosen as the three chief factors in PSAS preparation. The experimental results showed that the optimum preparation condition for various HAB organisms was different, and according to practical experimental operation, the appropriate preparation condition was considered as: concentration of SiO2 = 2%, molar ratio of Al3+/SiO2 = 1, and pH = 4. In addition, the coagulation efficiencies of PSAS on various HAB organisms were studied and compared with classical inorganic flocculant AS (Aluminium sulfate). The results indicated that the removal rates of PSAS were much higher than those of AS, and the dosages of PSAS were 30-40% lower than those of AS when they achieved the same removal rates. On the other hand, the anti-coagulation ability of HAB organisms varied with algal species, which was related to different physiological and ecological features of various algal cells.

Alum Compounds↗

PubMed2002

[Inhalation of sulfur dioxide on the DNA damage of brain cells in mice].

OBJECTIVE: To investigate the inhalation of sulfur dioxide (SO(2)) on the DNA damage of brain cells in mammalian animals. METHOD: Single cell microgel electrophoresis technique (comet test) was used to test the DNA damage of brain cells. RESULTS: (1) After the exposure to SO(2) at 0, 7, 14, 28 mg/m(3), the tail lengths of nuclear DNA in brain cells from male mice were 8.02, 23.14, 46.43 and 53.49 micro m respectively; and that from female mice were 7.23, 12.43, 20.39 and 54.83 micro m respectively. The results showed that: (1) SO(2) inhalation caused damage on DNA of brain cells in a dose-dependent manner; (2) Even under lower concentration of SO(2) as 7 mg SO(2)/m(3), the damage on DNA of brain cells was also reached to 98.8%. It implied that the brain cells of mammalian animals were very sensitive to SO(2) inhalation; (3) The DNA damage of brain cells from male mice is more serious than that from female mice. The reasons remain to be further studied. CONCLUSIONS: SO(2) pollution even at lower concentrations also had a potential risk to the genetic material DNA of brain cells from mammalian animals. The results of our study might explain the recently published epidemiological studies that the workers exposed to SO(2) or SO(2) derivatives had suffered an increase of mortality from brain cancer.

Administration, Inhalation↗

PubMed2002

[Identification of markers linked to resistance locus of Marssonina leaf spot in poplars by bulked segregant analysis(BSA)].

DNA markers linked to resistance locus of Marssonina leaf spot in poplars were found by bulked segregant analysis(BSA). The bulks consisted of individual with a extreme phenotype taken from a population of 91 F1 clones,which is a progeny of Populus deltoides Bartr.cv."Lux"(I-69/55)(Resistance) and P.euramericana cv.I-45(Susceptible). Out of 114 RAPD primers, four markers showed polymorphisms between the resistance-bulk and the susceptible-bulk.By using selective genotype linkage analysis,OPAI17-1550 and OPAI13-900 were found linked to the resistance locus. The genetic distances between the two markers and the resistance locus were 29.9cM and 37.4cM,respectively.

English Abstract↗

PubMed2001

Expression of Colonization Factor Antigen I of Enterotoxigenic Escherichia coli in Shigella flexneri 2a T32.

A host-plasmid lethal balancing system was constructed based on asd gene in an avirulent strain of S.flexneri to express colonization factor antigen I(CFA-I) of enterotoxigenic Escherichia coli.The results of Western blotting suggested that avirulant strain of S.flexneri Fwl01 expressed CFA-I steadily. Examination of negatively-stained preparation of cultures by electron microscopy showed that S.flexneri Fwl01 carrying the plasmid pZHY21 had thick pili on its surface. Antibodies against CFA-I were decteted in sera of mice immunized with recombinant bacteria either orogastrically (o.g.) or intranasally(i.n.); simultaneously sIgA against CFA-I was also decteted in the intestine. This work is helpful for constructing multivalent reombinant vaccine for prevention of bacterial diarrhea.

Journal Article↗

PubMedDate not supplied

Endoscopic transaxillary capsulectomy.

BACKGROUND: Traditionally, breast implant extraction and capsulectomy have been performed using a transareolar approach. However, this approach is not acceptable to Chinese patients because of the additional scar formation. The authors present their experience with capsulectomy using transaxillary endoscopic assistance without the need for an additional incision. METHODS: The former transaxillary incisional scar for augmentation mammoplasty is used. Blunt dissection is performed to the outer surface of the fibrous capsule. A 30 degrees , 10-mm endoscope is placed through the axillary incision to dissect the outer surface of the capsule. After this is finished, the capsule is cauterized open, and extraction is completed. Transaxillary capsulectomy is performed under endoscopic control. RESULTS: From 2003 to 2005, a total of 30 breasts involving 15 patients were treated. The follow-up period was 4 to 6 months, with favorable results. No scar was left on the breast, and no additional scar was made. No liquid accumulation was identified. CONCLUSIONS: Endoscopic transaxillary capsulectomy can be completed through the axillary incision. The technique successfully removed the fibrous capsule, eliminated the needed for an incision on the breast, and created an incision far from the breast for completion of the procedure.

Adult↗