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Biomedical subjects

Bing-sheng Yin

Publications and source records attributed to Bing-sheng Yin.

3 recordsLinked to original sources

[An efficient method for simulating ventricular electrical activity based on anatomic structure by incorporating AP model].

Conventional medical experiments can hardly simulate cardiac excitation propagation and observe the evolvement of cardiac electrical activities firsthand as is possible with computer simulation. Based on the anatomic structure of the heart, simulation of cardiac electrical activity mainly consists of the emulation of the excitation process among the cardiac cells and calculation of the electrical activities of individual cardiac cells. In this study we establish a geometric ventricular structure model demonstrating the direction of the cardiac muscle fibers and the layers of the ventricular cells, and endow different action potential models to the ventricular cells of different layers, and observe the activation process of the ventricular parts in view of the three-dimensional anatomy. This method gives attention to both enough calculation amounts and efficiency, which achieves satisfactory simulation results of ventricular electrical activity based on the anatomic structure and cell electrophysiology through an improved algorithm on personal computer.

Algorithms↗

[A new method for detecting serum beta-lipoprotein electrophoretogram].

OBJECTIVE: To establish a new method for isolating serum beta-lipoprotein (betaLp) subfractions using second double gradient polyacrylamide gel electrophoresis (s-DGPAGE). METHODS: After the betaLp subfractions were isolated by mechanisms of molecular sieving and isoelectric focusing due to density and pH grade in the non-linear density gradient polyacrylamide gel, the gels were acquired with a gel imaging system, the electrophoretograms were quantitatively analyzed with optical density scanner. The isolation of serum betaLp was completed through first DGPAGE. RESULTS: s-DGPAGE separated the serum betaLp into four subfractions, designated respectively as betaLp1, betaLp2, betaLp3 and betaLp4, and their amounts in the betaLp and in the whole lipoproteins could be quantified. CONCLUSION: s-DGPAGE is a simple and practical method to show the heterogeneity of serum betaLp and the quantitative variation of its subfractions.

Electrophoresis, Polyacrylamide Gel↗

Features of serum beta-lipoprotein electrophoretogram in patients with coronary heart disease.

OBJECTIVE: To investigate the changes in serum beta-lipoprotein (beta Lp) electrophoretogram in the patients with coronary heart disease (CHD). METHODS: This study included 36 CHD patients, 28 non-CHD patients and 8 normal control subjects, from whom serum beta Lps (prestained with Sudan Black B) were isolated by means of the first double-gradient polyacrylamide gel electrophoresis (f-DGPAGE). The secondary DGPAGE was performed for isolating beta Lps into 4 subfractions (beta Lp1-4) in order to complete the quantitative analysis of beta Lp electrophoretogram. A parallel test to examine the serum concentration of low-density lipoprotein cholesterol (LDLc) was also carried out. RESULTS: The relative amount of beta Lp1 was significantly elevated in CHD patients (53.4+/-10.0%) as compared with normal subjects (39.3+/-6.7%) and non-CHD patients (42.8+/-6.8%, P < 0.05 for both comparisons) regardless of serum LDLc concentrations, and beta Lp1 content between the latter 2 groups showed no significant difference. CONCLUSION: Serum beta Lp subfraction imbalance evidenced by increased beta Lp1 level characterizes the patients with CHD.

Adult↗