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Bennie H Jeng

Publications and source records attributed to Bennie H Jeng.

16 recordsLinked to original sources
PubMed2006

Narrow-strip conjunctival autograft for treatment of pterygium.

PURPOSE: To determine the efficacy of narrow-strip conjunctival autograft surgery in the treatment of pterygium. DESIGN: Retrospective noncomparative interventional case series. PARTICIPANTS: Twenty-one consecutive interventions between 1994 and 2005 in 20 eyes of 18 patients for primary (n = 17 cases) or recurrent (n = 4 cases) pterygia. INTERVENTION: Surgical excision of the pterygium and conjunctival transplantation with a narrow-strip technique. In all cases, a 2-mm-wide autograft was secured adjacent and posterior to the limbus, leaving a 2- to 3-mm zone of bare sclera between the graft and the anterior margin of the conjunctival wound. All conjunctival margins were secured by suture to bare sclera. MAIN OUTCOME MEASURE: Recurrence of pterygium at 12 months. RESULTS: Mean follow-up after surgery was 41 months (median, 42; range, 4-132). At 1 year and all time points thereafter, 18 of 19 (94.7%) cases were free of recurrence. One patient was lost to follow-up at 4 months, and another died after 9 months of follow-up. The lone recurrence occurred inferiorly in an eye that had undergone an adjacent narrow-strip conjunctival transplantation 6 months previously for a recurrent temporal pterygium. The same patient remains recurrence-free 67 months after inferior pterygium excision and a second narrow-strip autograft surgery. CONCLUSION: Narrow-strip conjunctival autografting appears to be an effective surgical technique in preventing pterygium recurrence. Creating an intervening bare sclera area between the secured conjunctival graft and the anterior margin of the conjunctival wound may be important in preventing recurrence.

Adult↗
PubMed2006

Rapid species determination of Nocardia keratitis using pyrosequencing technology.

PURPOSE: To describe a new technique, pyrosequencing, which allows for the rapid identification of Mycobacterium and Nocardia species. DESIGN: Interventional case report. METHODS: The medical records of a patient presenting with an infectious keratitis were reviewed. RESULTS: A case of Nocardia abscessus/arthrititis/asiatica keratitis was diagnosed in a young individual with the aid of pyrosequencing technology. Based on presumed antibiotic sensitivities, therapy with topical trimethoprim-sulfamethoxazole eyedrops was initiated, and the infection was cleared rapidly with minimal residual scarring. CONCLUSIONS: Pyrosequencing may be a useful tool in aiding the rapid diagnosis and treatment of ocular infections caused by slow-growing pathogens.

Adult↗
PubMed2006

Bacterial culture isolates from hospitalized pediatric patients with conjunctivitis.

PURPOSE: To determine the spectrum of organisms causing acute bacterial conjunctivitis in hospitalized children at a tertiary care referral center. DESIGN: Retrospective chart review. METHODS: Charts of hospitalized children with positive conjunctival cultures were reviewed, and patients with clinical description of conjunctivitis were studied. RESULTS: One hundred and seven isolates from 59 patients were included in the study. The most common organisms cultured were coagulase-negative Staphylococcus (59.3% of patients), viridans Streptococcus (47.5%), and Staphylococcus aureus (20.3%). The type of organisms differed based on age, with S. aureus and Haemophilus influenzae being more common in nonneonates. Gram-negative bacilli and methicillin-resistant Staphylococcus species were more common in patients hospitalized longer than two days. CONCLUSIONS: The distribution of bacterial organisms causing acute bacterial conjunctivitis in our hospitalized children differs from that of previous reports in the outpatient setting. Conjunctival swabbing for culture and sensitivities before instituting empiric broad-spectrum antibiotic therapy may be useful.

Acute Disease↗
PubMed2006

Reduction of hyperopia and astigmatism after superficial keratectomy of peripheral hypertrophic subepithelial corneal degeneration.

PURPOSE: To report the development of progressive hyperopia and astigmatism resulting from peripheral hypertrophic subepithelial degeneration which was treated with superficial keratectomy of the lesion. METHODS: Case report. RESULTS: A patient with peripheral hypertrophic subepithelial degeneration of both corneas was followed up for a 6-year period. During this time, the patient experienced a slowly progressive increase in hyperopia and astigmatism in both eyes. In the left eye, extension of the degeneration paracentrally resulted in significant hyperopia and astigmatism and a best spectacle-corrected visual acuity of 20/70. Superficial keratectomy was performed and resulted in a dramatic reversal of the hyperopic and astigmatic shift and a best spectacle-corrected visual acuity of 20/30. CONCLUSIONS: Peripheral hypertrophic subepithelial corneal degeneration can result in progressive flattening of the central corneal topography in the involved meridians. This flattening can induce a significant hyperopic and astigmatic shift in refraction. This refractive change can be reversed with superficial keratectomy to remove the degeneration.

Aged↗
PubMed2006

Superficial hypertrophic dendritic epitheliopathy occurring before and after penetrating keratoplasty in a patient with psoriasis.

PURPOSE: To report a patient with superficial hypertrophic dendritic epitheliopathy that occurred in 1 eye after keratoplasty and in the other eye that had not undergone previous corneal transplantation. In both eyes, the epitheliopathy recurred even after subsequent penetrating keratoplasties. METHODS: Case report. RESULTS: A patient with chronic eyelid and ocular surface disease secondary to psoriasis was monitored over a 17-year period. During this time, the patient developed a superficial hypertrophic dendritic epitheliopathy of the left eye 3 years after corneal transplantation. In the right eye, which had not undergone a penetrating keratoplasty, the patient was also noted to have a superficial hypertrophic dendritic epitheliopathy that changed morphologies over the ensuing 5.5 years. Both eyes subsequently required penetrating keratoplasties for decreased vision secondary to subepithelial scarring. After surgery, both corneas suffered recurrences of the superficial hypertrophic epitheliopathy. CONCLUSION: In addition to the original findings that superficial hypertrophic dendritic epitheliopathy occurs in postkeratoplasty eyes, this condition can also occur in corneas that have not been transplanted previously. The morphology of these lesions can vary.

Adult↗
PubMed2006

Preserving the cornea: corneal storage media.

PURPOSE OF REVIEW: The aim of this article is to review the recent literature regarding corneal storage media since the last review that discussed this topic in substantial detail in this journal. RECENT FINDINGS: During the last few years, despite the development of new corneal storage media and the addition of new additives to established corneal storage media, Optisol GS (Bausch & Lomb, Irvine, California, USA) has continued to remain the popular choice among storage media used in the United States, and traditional organ culture methods are still used in Europe. Recognizing that persistent epithelial defects after corneal transplantation can be a serious complication, however, attention has started to focus on not only preserving the endothelium, but also the epithelium. In addition, there has been more research towards antimicrobial prophylaxis. SUMMARY: With recent evidence suggesting that longer storage times may allow better outcomes in high-risk grafts because of the depletion of donor T cells from the donor cornea into the storage media, storage media will need to be optimized for preserving the endothelium for longer periods of time. In addition, because the epithelium is typically unable to be sustained for longer than 1 week in storage media, research toward preserving the epithelium will also be essential.

Cornea↗
PubMed2005

Immunohistochemical detection and Western blot analysis of nitrated protein in stored human corneal epithelium.

While the production of nitric oxide by human corneas in storage has recently been demonstrated, protein nitration as a result of this production has not been demonstrated. In this study, nitrated protein accumulation in the epithelium of stored human corneas was assessed. One half of five donor corneas maintained in storage media for 3 days were prepared for immunohistochemical studies. The other halves remained in storage media for 7 additional days and were also processed for immunohistochemistry. Mouse monoclonal antibody to nitrotyrosine adducts was used to define the localisation of these epitopes. The density of antibody staining was observed and quantified on a digital camera system and statistically analysed. Immunostaining in the epithelium was greater in tissues recovered after 10 days in storage compared to the intensity of staining after 3 days of storage (p<0.0001). No staining was evident in the epithelium in sections exposed to non-immune mouse IgG. Western blot analysis was performed on epithelial cells scraped from corneal surfaces of one-half of four donor corneas in storage for 3 days and from the other half at 10 days of storage. Nitrated BSA was used as a positive control. After extraction and homogenisation, identical protein concentrations of each sample were loaded per lane on 10% gels and subjected to SDS-PAGE. Proteins were blotted and probed with the anti-nitrotyrosine antibody. Western blot immunoreactivity was detected in epithelial samples at the 3 and 10 day recovery times with the latter samples showing greater staining intensity. Nitrated protein, thought to indicate toxic peroxynitrite formation, accumulates in the human corneal epithelium with time of storage. Our study shows that there is an association between increased nitrated protein and storage time.

Adult↗
PubMed2004

Inhibition of nitric oxide synthesis in corneas in storage media.

The nitrate/nitrite content in storage media was determined after nitric oxide synthase inhibition by adding 400 microl of 100 mm N(G)-monomethyl-l-arginine (LMMA) to four chambers of Optisol GS corneal storage media, each containing one viable human cornea. The companion corneas in storage media without LMMA served as controls. Four hundred microlitre aliquots obtained at baseline (day 0) and at one-day intervals for 20 more days for both groups were analyzed for nitrate and nitrite (breakdown products of nitric oxide) concentration levels using a spectrophotometric method based on the Greiss reaction. Average nitrate/nitrite concentrations, statistically analyzed using a polynomial random coefficients model, showed a statistically significant marked reduction in the levels of nitrate and nitrite accumulation in the study chambers as compared to control chambers for days 1-20(P < 0.001) There was also a reduction in the accumulation rate of nitrate and nitrite concentrations, as compared to controls (P < 0.05) until around day 8 when the differences in rates were no longer statistically significant. The progressive increase in nitrate and nitrite accumulation in corneal storage media can be blunted by the addition of a nitric oxide synthase inhibitor. Given the toxic free radical properties of nitric oxide, corneas in storage awaiting transplantation may benefit from having a nitric oxide synthase inhibitor added to storage media.

Cornea↗
PubMed2004

Completion rate of continuous curvilinear capsulorhexis in pediatric cataract surgery using different viscoelastic materials.

PURPOSE: To determine whether viscoelastic materials with a higher viscosity allow better control of the lens capsule, reducing the rate of incomplete continuous curvilinear capsulorhexis (CCC) during pediatric cataract surgery. SETTING: Department of Ophthalmology, University of California, San Francisco Medical Center, San Francisco, California, USA. METHODS: This retrospective study comprised 25 eyes of 18 children who had cataract surgery by 1 surgeon. The type of viscoelastic agent used for the CCC portion of the surgery was obtained from surgical dictations and nursing charts and information on complications during the CCC, from surgical dictations. The patient's age, type of cataract, and intraocular lens placement were also obtained. RESULTS: A CCC was successfully performed in 7 of 15 eyes (46.7%, mean age 7.6 years) using cohesive Healon (sodium hyaluronate 1%) or dispersive Viscoat (sodium hyaluronate 3%-chondroitin sulfate 4%) viscoelastic material and in 9 of 10 eyes (90%, mean age 6.4 years) using the high-viscosity viscoadaptive agent Healon 5 (sodium hyaluronate 2.3%) (chi square = 4.8900; P<.05). The unsuccessful CCCs in the Healon/Viscoat group were in 2 eyes with mature cataract, 1 eye with posterior polar cataract, 1 eye with posterior subcapsular cataract, and 4 eyes with lamellar cataract. The unsuccessful CCC in the Healon5 group was in 1 eye with mature cataract. CONCLUSION: The superviscous properties of Healon5 under low turbulence conditions appear to lead to a higher CCC completion rate in children.

Adolescent↗
PubMed2004

Pseudogerontoxon.

Pseudogerontoxon is a lesion that resembles a small segment of arcus senilis or gerontoxon and is seen in many individuals with limbal vernal or atopic keratoconjunctivitis. It is an important clinical finding because pseudogerontoxon is often times the only clinical evidence of previous allergic eye disease. To the authors' knowledge, pseudogerontoxon has only rarely been mentioned, and has never been pictured, in the peer-reviewed literature. Three examples are presented of this important clinical entity.

Adult↗
PubMed2002

Nitric oxide generated by corneas in corneal storage media.

PURPOSE: The purpose of the study was to quantify nitric oxide release by human corneal buttons in storage media over time. METHODS: Group 1 consisted of six chambers of Optisol GS corneal storage media, each containing a viable human corneal button with an attached scleral rim (unsuitable for transplantation), sampled at 1-day intervals for at least 17 days (range, 17-28 days). Group 2 consisted of 34 chambers of Optisol GS media, each used to store a corneal button for penetrating keratoplasty, sampled immediately after each surgery. An unused vial of Optisol GS storage medium was sampled daily for 17 days to serve as a background medium control. The total amount of nitrite and nitrate in each sample was determined by a spectrophotometric method based on the Griess reaction. RESULTS: Data from the daily sampling in group 1 showed that nitrite and nitrate concentrations in storage media containing human corneas increase from a baseline level (beginning at the time the corneas are placed in the media) to an equilibrium concentration of 2.77 microM in a mean time of 6.15 days. Seventy-six percent of the data points from group 2 fell within the 80% predictive interval derived from group 1. No nitrite or nitrate was detected in background medium control samples. CONCLUSION: The progressive increase in nitrite and nitrate in corneal storage media over time suggests that nitric oxide is continuously released by corneas during storage before transplantation. Given the toxic free radical properties of nitric oxide, corneas in storage media may be subjected to the cumulative toxic effects of nitric oxide.

Chondroitin Sulfates↗
PubMedDate not supplied

A combined technique for surgical repair of Descemet's membrane detachments.

BACKGROUND AND OBJECTIVE: Descemet's membrane detachments are an uncommon complication after cataract surgery that can result in severe visual loss. A combined technique of intracameral gas injection and transcorneal suturing for the repair of Descemet's membrane detachments is described. PATIENTS AND METHODS: In this interventional case series, four cases of Descemet's membrane detachments with associated corneal edema observed following cataract surgery were successfully repaired using a combined technique of intracameral gas injection and transcorneal suturing. RESULTS: In all cases, Descemet's membranes were successfully reattached using the aforementioned technique. In three cases, the associated corneal edema resolved postoperatively. In the fourth case, the patient required a penetrating keratoplasty for persistent corneal edema despite immediate anatomical success following reattachment. CONCLUSIONS: Combined intracameral gas and transcorneal suturing appears to be an effective technique in the repair of Descemet's membrane detachments. Early intervention may prevent persistent or recurrent corneal edema.

Aged↗
PubMedDate not supplied

Late-onset Corynebacterium endophthalmitis following laser posterior capsulotomy.

Four months following uncomplicated cataract extraction, a patient underwent Nd:YAG laser posterior capsulotomy. Three days later, she presented with pain, hand motions vision, and severe anterior uveitis and vitritis. A coincident retinal detachment led to a delay in diagnosing the etiology of this intraocular inflammation. After recurrent episodes of inflammation that were initially responsive to corticosteroids, the patient underwent a vitrectomy, lens explantation, capsulectomy, and intravitreal antibiotic injections, which resulted in complete resolution of the intraocular inflammation with a best-corrected visual acuity of 20/60. Corynebacterium species was ultimately cultured from the capsular tissue. The release of sequestered bacterial organisms must be considered in the differential diagnosis of persistent or unusually intense inflammation following laser posterior capsulotomy.

Aged↗