Biomedical subjects

Ben Davidson

Publications and source records attributed to Ben Davidson.

77 records · Page 5Linked to original sources

PubMed2002

Caveolin-1 expression in ovarian carcinoma is MDR1 independent.

We studied the role of caveolin-1 in tumor progression and prognosis in serous ovarian carcinoma and the association between caveolin-1 and MDR1 expression. The study involved immunohistochemical analysis for caveolin-1 and P-glycoprotein (P-gp) expression in 75 effusions and 90 solid lesions from ovarian and primary peritoneal carcinoma; in situ hybridization for MDR1 messenger RNA (mRNA) expression in 62 effusions and all 90 tumors; and reverse transcription-polymerase chain reaction (RT-PCR) for caveolin-1 mRNA expression in 23 effusions. Immunohistochemical analysis localized caveolin-1 to the cell membrane in 43 effusions and 24 tumors. P-gp membrane expression was detected in 14 effusions and 11 tumors; MDR1 mRNA, in 20 effusions and 30 tumors. Caveolin-1 mRNA was expressed in 19 effusions. Caveolin-1 protein expression showed no association with that of P-gp protein or MDR1 mRNA. The expression of all markers was similar in carcinoma cells in pleural and peritoneal effusions. Caveolin-1 is a novel diagnostic marker for effusions; expression is moderately elevated in tumor cells in effusions, possibly owing to altered signal transduction and metabolism in cancer cells at this site. Expression seems MDR1 independent.

ATP Binding Cassette Transporter, Subfamily B, Mem↗

PubMedDate not supplied

Malignant mesothelioma.

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Diagnosis, Differential↗

PubMedDate not supplied

Cadherin expression in ovarian carcinoma and malignant mesothelioma cell effusions.

OBJECTIVE: To analyze potential differences in cadherin expression between ovarian carcinoma/primary peritoneal carcinoma (OC/PPC) and malignant mesothelioma (MM) at this anatomic site. STUDY DESIGN: MM (N=24) and OC/PPC (N= 53) effusions were analyzed for E-cadherin, N-cadherin and P-cadherin protein expression using immunocytochemistry. RESULTS: Both MM and OC/PPC cells showed frequent expression of all 3 cadherins. OC/PPC specimens expressed E-cadherin and N-cadherin in 52 of 53 cases and P-cadherin in 51 of 53 cases. MM effusions expressed E-cadherin, N-cadherin and P-cadherin in 22 of 24, 21 of24 and 23 of24 cases, respectively. The differences in the percentage of cadherin-positive cells was weakly significant for P-cadherin (higher expression in MM, p = 0.04), but E-cadherin and N-cadherin expression was comparable (p > 0.05). CONCLUSION: MM and OC/PPC coexpress different cadherin family members. P-cadherin, E-cadherin and N-cadherin are not useful for differentiation between OC/PPC and MM in effusions.

Ascitic Fluid↗

PubMedDate not supplied

Dear CNO.

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Humans↗

PubMedDate not supplied

The biological role and regulation of matrix metalloproteinases (MMP) in cancer.

Matrix metalloproteinases (MMP) are zinc-dependent proteolytic enzymes capable of breaking down basement membranes and most extracellular matrix (ECM) components. MMP expression and activation are carefully regulated in physiological conditions in order to prevent uncontrolled destruction of body tissues but this regulation is modified or disrupted in pathological processes, including cancer. This review presents regulatory mechanisms designed to control MMP action. These consist of direct activation and inhibition by tissue inhibitors of MMP (TIMP), signal transduction pathways mediated by adhesion molecules such as integrins and EMMPRIN, involved in activation of MMP synthesis and transcriptional control by the ETS family of transcription factors.

Animals↗