Biomedical subjects
Publications and source records attributed to B Z Itkin.
The level of large granular lymphocytes (LGL) has been determined in thoracic lymph duct and peripheral blood of cattle. It has been shown that in contrast to the blood, these cells are present in the lymph in minor quantities. Unlike blood LGL, lymph LGL have smaller granules in cytoplasm. It is concluded that LGL do not recirculate from blood to central lymph.
The composition and structure of the neutral glycolipids of calf thymus and of lymphocytes isolated from the blood, lymph, lymph nodes and spleen of normal and leukemic cows were investigated. The main neutral glycolipids of all lymphocytes studied were identified as glucosyl, lactosyl and digalactosylglucosylceramides. The relative amounts of these glycolipids were found to depend on lymphocyte localization. Leukemic lymphocytes showed differences in the glycolipid composition in comparison with normal lymphocytes: in all preparations the level of glucosylceramide increased with a concomitant decrease of the amounts of di-and trihexaosylceramides. For leukemic blood lymphocytes the monohexaosyl-/dihexaosylceramide ratio was found to increase at increasing lymphocyte concentrations in the blood.
The phospholipid composition and cholesterol content of lymphocytes from bovine blood, lymph, lymph-nodes and spleen were studied. The cholesterol/phospholipid molar ratio in the normal lymphocytes of blood and spleen was found to be about two times higher than that in the normal lymphocytes from lymph and lymph-nodes. On lymphocytic leukemia the cholesterol/phospholipid molar ratio of the blood lymphocytes decreased sharply, whereas that in lymph and lymph-nodes lymphocytes was unaffected.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
A model was constructed of accumulation kinetics of labeled lymphocytes based on the experiments of long-term injection of 3H-thymidine label in vivo into the blood of healthy and suffering from chronic lympholeucosis animals. There was found an essential difference between the coefficients of reproduction and death of cells of the proliferating pool for the normal, initial and advanced stages of the disease. This served as a basis for the creation of the closed non-linear model of autoregulation of lymphoid population size describing different stages of leucosis development.
Proteolytic activity was estimated in lymphocyte lysates of cattle in normal state and in chronic lympholeukosis using 3H-acetylated casein (pH 7.4) and 3H-acetylated hemoglobin (pH 4.0) as substrates. Distinct individual variations in the enzymatic activity were observed either in neutral or slightly-acid media in the animal groups studied. In chronic lympholeukosis specific proteolytic activity, calculated per a cell, was decreased, while it was increased in calculation per a mg of protein. The lower content of protein (about 2.4-fold) was found in lysates of lymphocytes in chronic lympholeukosis as compared with normal state. An increase in proteolytic activity in lymphocyte lysates correlated with elevation of blood leukocytosis if chronic lympholeukosis developed in individual animals. Effects of a number of proteinase inhibitors and activators were studied in the cell lysates; the spectrum of proteinases in chronic lympholeukosis was dissimilar to that of normal state. Pepstatin inhibited quite completely the proteolytic activity at pH 4.0 in lymphocytes of healthy animals and hence cathepsin D was responsible for the activity; in chronic lympholeukosis, except of cathepsin D, thiol-dependent proteinase was also detected. Activity of proteinases at neutral pH value in lymphocytes of healthy and impaired animals was inhibited by phenylmethionine fluorosulfate and p-chloromercuribenzoate, thus suggesting that serine and thiol-dependent proteinases were present; the level of these enzymes was distinctly higher in chronic lympholeukosis as compared with normal state.
Explore the source record for details and available documents.
Comparison was made of the rate of in vivo elimination from blood and organs of cattle of lymphocytes labeled with 3H-thymidine in the course of seven days in the norm and during lymphatic leukemia. Significant delay in the elimination of labeled lymphocytes, and especially of lymphocyte precursors, from the blood, spleen, and lymph glands of the animal was established in the stage of lymphatic leukemia that was examined.
It was established that in healthy animals the intensity of synthesis of quick-labeling RNA and also the percentage of small and medium labeled lymphocytes are substantially higher in the lymphocytes of lymph than in the lymphocytes of blood. The percentage of small blood lymphocytes doubles (from 25 to 50%) during leukosis, but is almost unchanged in lymph. For the majority of animals with leukosis, an increase of 5 to 10 times in the percentage of strongly labeled cells, including all types of lymphocytes, is characteristic.
Explore the source record for details and available documents.
Explore the source record for details and available documents.