Electrochemical behaviors of quercetin and kaempferol in neutral buffer solution.
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Buffers↗
Biomedical subjects
B Yang
Publications and source records attributed to B Yang.
Suicide notes of Chinese youth.
Examination of 17 suicide notes from youthful Chinese suicides indicated the common appearance of anger, self-criticism, and interpersonal friction as precipitant.
Adolescent↗
Suicide as an indicator of quality of life: apparent conflict between microeconomic and macroeconomic approaches.
While a low incidence of suicide may indicate a high quality of life for individuals, the reverse may be true at the societal level.
Depression↗
Predicting the number of credit cards held by college students.
In a sample of 186 undergraduate students, the number of credit cards they held was associated with sex, their affective attitude toward credit cards, and an attitude of being careful with money.
Adolescent↗
[Effects of heat shock protein 70 overexpression on apoptosis of K562 cell caused by hyperthermia].
In order to observe the effects of heat shock protein 70(HSP70) on the apoptosis of K562 cell caused by hyperthermia, HSP70 was induced by pre-heating K562 cells at 40 degrees C and the levels of HSP70 were detected by RT-PCR. Cell apoptosis caused by hyperthermia at 43 degrees C was observed by Fluid cytometry (FCM) DNA content analyses. The results showed that HSP70 increased with the prolong of pre-heating time and reached the top level on pre-heating for 120 min and lasted for several hours. A lower apoptosis rate and less DNA ladder forming in the pre-heated cells than those not pre-heated. It is concluded that the higher expression of HSP70 induced by pre-heating treatment could inhibited the apoptosis caused by hyperthermia.
Apoptosis↗
[Cloning and expression of TPS1 gene in Escherichia coli].
A S. cerevisiae TPS1 gene for trehalose-6-phosphate synthase was cloned by PCR amplification. The 1.5 kb DNA fragment was ligated to pUC19 and transformed into otsA deficient and deleted of E. coli strains FF4169 and FF4050 separately. otsA gene is encoding trehalose-6-phasphate synthase in E. coli. Restriction endonucleases digestion analysis of transformants' plasmid DNA showed that there was a cloned 1.5 kb fragment carried on the vector. The growth curve experiment result showed that the both transformants could grow well as wild type. Trehalose was synthesized and accumulated in these transformants during high osmotic stress by HPLC combined with ELSD (Evaporate light scatter detector) determination. From the result above, we could conclude that the TPS1 gene of S. cerevisiae was able to restore otsA gene function in E. coli for both osmotolerance and trehalose accumulation during salt stress.
Cloning, Molecular↗
[A design of Hospital Information System].
This paper describes a design for Hospital Information System on the basis of local area networks.
Computer Systems↗
[Combustion characteristics of municipal solid waste in fluidized bed].
For the experimental investigation on the combustion characteristics of Municipal Solid Waste(MSW) in Fluidized Bed, a lab scale Fluidized Bed facility was constructed. Single and mixed municipal solid waste burning in Fluidized Bed showed that dry waste can be burnt quickly at the bed temperature of 500 degrees C, and furnace temperature increased about 30-50 degrees C. Many kinds of combustion runs were conducted in this Fluidized Bed combustion facility. The parameters examined were air flow rate(from 5.5 m3/h to 7.5 m3/h), form of fuels(scrap or whole), moisture content and so on. Concentration of CO2, CO, SO2 and NOx in the flue gas were monitored and recorded every 5 seconds. The temperatures along the reactor were recorded every 10 seconds.
Hot Temperature↗
The therapeutic effects of Sheng Jing Zhong Zi Tang in treating male infertility.
Eight-seven cases of male infertility due to spermatopathy were treated with Sheng Jing Zhong Zi Tang (SJZZT [symbol: see text]) for 1-3 courses. The total effective rate was 95.40% (83/87). 56.32% (49/87) of their spouses got pregnant and the semen quality was markedly improved (P < 0.01). The results showed that the decoction could dual-directionally regulate the levels of follicle-stimulating hormone (FSH), prolan B luteinizing hormone (LH), testosterone (T), and cortisol (C).
Adult↗
Inhibitory effect of nomegestrol acetate on steroidogenesis of cultured granulosa cells from rat ovary in vitro.
AIM: To study the effect of nomegestrol acetate, a new synthetic progesterone on granulosa cells' viability and steroidogenesis function. METHODS: Granulosa cells were cultured in McCoy's 5A medium. Trypan blue stain was used to measure viable cells. FSH and testosterone were added to stimulate the steroid secretion. Specific RIA assay was used to evaluate the estrogen and progesterone secretion respectively. RESULTS: IC50 of nomegestrol acetate to damage cells is 6.85 mg/L (95 % confidence limits 5.36-8.75 mg/L). Nomegestrol acetate 0.45, 0.9, and 1.8 mg/L greatly inhibited the estrogen secretion from granulosa cells by 7.6 %, 12.5 %, 28.3 % in the presence of testosterone 0.5 micromol/L and FSH 10 U/L without affecting the number of viable cells. The secretion of progesteron were markedly decreased by 44.5 %, 53.3 %, and 62.0 % concurrently. CONCLUSION: Nomegestrol acetate directly inhibited the steroidogenesis of granulosa cells.
Animals↗
[Microscopic measurement of the surgical resection margins of laryngeal carcinomas].
OBJECTIVE: To investigate the relationship between the primary sites of laryngeal carcinomas and the surgical resection margins, to obtain quantitative data of the safe distance of surgical margins for supraglottic and glottic carcinomas. METHODS: In this series, 33 cases of surgical specimens of laryngeal carcinomas were examined and measured microscopically by means of cutting strips of tissues adjacent to the tumor and longitudinal serial sections. RESULTS: The pattern and extent of invasion of supraglottic carcinoma were quite different from those of glottic carcinoma. Under microscopic measurement, the tumor invasion distance in 95% of the supraglottic carcinoma samples was within 10.52 mm, while that of 95% of the glottic carcinoma samples was within 4.41 mm. CONCLUSIONS: The local invasion of laryngeal carcinomas appears to be primary tumor site-dependent. The safe distance of surgical resection margins may be set at 12.52 mm and 6.41 mm for supraglottic and glottic carcinomas, respectively.
Adult↗
[The bone regeneration in cleft palate after surgical repair (a prelimilnary observation)].
OBJECTIVE: To study the bone healing situation of the cleft palate after surgical procedure, and discuss the factors affecting it. METHODS: 20 patients with repaired cleft palate were examined by CT scan coronally. The result of images were observed and the position, shape and quality of bone bridge were measured. RESULTS: Formation of bone bridge was found in 13 cleft gaps out of 20 patients (65%), the sex ratio among patients with bone bridge was 1:1; there was no obvious difference between unilateral and bilateral cleft palate. Considering the operation age with the bone formation, the highest percentage of bone bridge formation fell into the group of 4-6 years old, and the most occurring regions were in premolar and anterior part of molar area. CONCLUSIONS: There can be new bone bridge regeneration in bone gap after palate surgery, and the bone formation may have some relationship with the operation age and surgical technique.
Adolescent↗
[Studies on quality specification standards for Saposhnicovia divaricata (Turcz.) Schischk].
OBJECTIVE: To work out a qualitative-quantitative standard for Saposhnikovia divaricata. METHOD: TLC and HPLC were adopted. RESULT: Four chromone compounds were identified by TLC, and the contents of two chromone compounds were determined by HPLC. CONCLUSION: Prim-O-glucosylcimifugin, 4'-O-beta-D-glucosyl-5-O-methylvisamminol, sec-O-glucosylhamaudol and cimifugin should be present simultaneously in S. divaricata. The contents of prim-O-glucosylcimifugin and 4'-O-beta-D-glucosyl-5-O-methylvisamminol in S. divaricata should not be lower than 0.12% respectively.
Apiaceae↗
[Studies on chemical constituents from root of Saposhnikovia divaricata (Turcz.) Schischk].
OBJECTIVE: To study the chemical constituents in the root of Saposhnikovia divaricata. METHOD: The compounds were isolated and identified by column chromatography and 1H, 13C NMR data. RESULT: Twenty-four compounds were isolated and identified. CONCLUSION: Seven compounds were isolated from this plant for the first time.
Adenosine↗
[Detection of trehalose in transgenic tobacco by HPLC with ELSD].
The E. coli trehase synthalose gene(otsA) was transferred into Nicotiana tabacum mediated by Agrobacterium, but the method for detecting low concentration of trehalose in transgenic plant was not available. The high performance liquid chromatograph(HPLC) with evaporative light-scatting detector(ELSD) using water: methyl cyanide(1:2.6 v/v) as mobile phase was established in this work. An ODS column Zorbax RX-SIL was employed. the trehalose detection limits of ELSD was 5 mg/L. From the linear relationship between the logarithm of trehalose concentration and the logarithm of peak area, it was shown there was 14.7 micrograms.(g FW)-1 in transgenic plant. The data strongly confirmed that trehalose was responsible for the improved stress tolerance of the tobacco.
Chromatography, High Pressure Liquid↗
[Expression of otsA gene in tobacco and improvement stress tolerance].
The Escherichia coli trehalose-6-phosphate synthase gene(otsA) was engineered under the control of cauliflower mosaic virus regulatory sequences (CaMV35S) for expression in plants. OtsA gene was incorporated into the chromosome DNA by Agrobacterium-mediated transfer and expressed in Nicotiana tabacum. The otsA gene transgenic plant exhibited multiple phenotypic alteration: improved stunted growth and drought tolerance. The detached leaves from the transgenic plant, their water loss slower than that of the controls. Trehalose accumulated in transgenic plant was determined by HPLC suggest that synthesis of the sugar improving plant to stress tolerance.
Gene Expression↗
[Non-linear compression for noises in non-contact vital signs detecting system].
A method for compressing the noises overlapping in the breath and heart signals of human beings, detected by the non-contact vital signs detecting system during the display of the waveforms in time domain, is discussed in this paper in detail. And what's more, the problem that the noise level is changed along with the increase of the gain of AD data-acquisition card and the display--gain of the software is solved by researching into the threshold of noise-level contraction in two types of circumstance in the lab, and the result of the breath and heart signal in the condition of lower noise is realized as well.
Artifacts↗
[The study on non-contact detection of breathing and heartbeat based on radar principles].
The paper introduces the design of hardware and software of non-contact detection system for breathing and heartbeat in human body with radar principles and technology. The detection technology is discussed. Under conditions of the illuminating power P < 1 mW and the distance S < 10 m, the non-contact breathing and heartbeat measurement, can be in different positions and with different clothing on the subject. The results show that the system with the technology has a high sensitivity, and is harmless to the health. It is a practicable non-contact detection technology for breathing and heartbeat of human body.
Adult↗