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Biomedical subjects

B Wilson

Publications and source records attributed to B Wilson.

At least 127 records · Page 7Linked to original sources
PubMed1991

Comparison of commercially available cytokeratin antibodies in normal and neoplastic adult epithelial and non-epithelial tissues.

Five commercially available cytokeratin antibodies (lu-5, AE1/AE3, CAM 5.2, MFN116 and anti-cytokeratin 18) were used to stain a wide range of normal and neoplastic epithelial and non-epithelial tissues to assess their potential value in diagnostic histopathology. All five showed good specificity, with some cross-reactivity in smooth muscle cells. The wider reactivity of AE1/AE3, lu-5, and MFN 116, which includes cytokeratins 8,18 (Moll's catalogue) expressed in simple epithelia and their tumours, as well as cytokeratins expressed in complex stratified squamous epithelia, permits identification of a wider range of epithelial derived tumours. This wider spectrum of reactivity may allow these antibodies to be used in a diagnostic panel for the identification of poorly differentiated tumours.

Antibodies, Monoclonal↗
PubMed1991

Performance of age-matched controls on a battery of visuo-spatial neglect tests.

Examination of 47 independent elderly subjects, matched with a population of patients with acute stroke, found that 55% made at least one omission on a battery of neglect tests. Up to 43% made omissions on any one test. Increasing age and other evidence of cognitive impairment were associated with impaired performance on the battery. Omissions were attributed to an age-related decline in visuo-spatial function. Cut-off points are provided to distinguish between such age-related impairment and visuospatial neglect. The importance of age-matched control studies in developing tests of cognitive impairment in stroke research is highlighted.

Adult↗
PubMed1991

The assessment of visuo-spatial neglect after acute stroke.

Forty four consecutive patients with acute hemispheric stroke and forty seven elderly controls with no neurological disease were assessed for visuo-spatial neglect, using a modified neglect test battery. Neglect was found to be equally common in patients with right hemisphere and left hemisphere stroke three days after stroke (72% versus 62%). It was more severe in those with a right hemisphere stroke and resolved more frequently in those with a left hemisphere stroke. The battery was validated against an occupational therapist's assessment of neglect on self-care tasks. The inter-observer reliability was good and it was possible to monitor changes over time with the battery.

Activities of Daily Living↗
PubMed1991

The effect of drugs on male sexual function and fertility.

Drugs may have negative effects on male libido, erection, ejaculation and orgasm, as well as on fertility, and research on these effects is increasing. Libido may be decreased by drugs that block dopamine or testosterone, or that cause dysphoria. Erection may be decreased by drugs that divert blood flow from the penis, or drugs that affect spinal reflexes. Ganglion blockers may also inhibit erection. Ejaculation may be diminished by drugs that affect spinal reflexes or be inhibited by ganglion blockage. Enervation of the vas deferens and epididymis may be blocked and cause a smaller emission. Retrograde ejaculation may occur due to blockage of the internal urethral sphincter. Orgasm is usually inhibited by the drugs that inhibit ejaculation. Fertility is impaired by drugs that affect sexual performance or spermatogenesis. Major groups of drugs that may affect male sexual function include drugs of abuse, CNS depressants, antihypertensives, anticholinergics, psychotherapeutics, hormones, and cancer therapeutics, in addition to miscellaneous other agents. Information about these drugs has been arranged in tables so that the provider has a convenient reference to use when explaining to men the effect of drugs on sexual response and fertility.

Drug-Related Side Effects and Adverse Reactions↗
PubMed1990

A method for delivery of precise doses of smoked cocaine-base to humans.

Despite increasing smoked cocaine-base use, there have been relatively few parametric studies in this area. The major reason for the limited number of studies is the lack of a simple procedure for the administration of precise doses of smoked cocaine-base to human volunteers. This paper describes a new method that allows for the delivery of precise doses of smoked cocaine-base. A complete description of the method and the precision of the administration procedure are presented. Furthermore, a study is described which was undertaken to determine: 1) the reproducibility of peak blood cocaine levels when the same dose of cocaine was given on two separate occasions; and 2) the dose-related effects on smoking topography, biochemical, physiological and subjective measures. Subjects (N = 5) were administered three doses of cocaine-base (10, 20 and 40 mg). Four subjects were given repeated doses of cocaine-base. Subjects were blind to the dose and in most cases randomly assigned to different doses. The results showed: 1) a significant correlation of peak whole blood cocaine concentrations among similar doses within subjects (r = .99); 2) no significant effects of dose on smoking topography; and 3) significant dose effects for whole blood cocaine concentrations, heart rate and systolic blood pressure.

Adult↗
PubMed1990

Children's frequency of participation in moderate to vigorous physical activities.

Third and fourth grade boys (n = 422) and girls (n = 390) in four Texas elementary schools reported their participation in moderate to vigorous physical activities (MVPAs) over a 3-day period. Students were surveyed during class on successive days. On a subsample (n = 44), the agreement between reported and observed physical activities during physical education or recess was 86.3%. Running, walking fast, games and sports, and bicycling accounted for 70% of Total MVPAs. Of Total MVPAs reported, 47.0% for boys and 44.6% for girls were 10 min or longer in duration (LMVPA). The average number of LMVPAs per day was 1.7 for both boys and girls. Students reported significantly more occurrences of LMVPAs out of school than during school. Significant interaction between grade and gender indicated that third grade boys reported more Total MVPAs and LMVPAs than third grade girls, but fourth grade boys reported fewer Total MVPAs and LMVPAs than fourth grade girls reported fewer Total MVPAs and LMVPAs than fourth grade girls. During the 3-day reporting period, 12.3% of boys and 13.3% of girls reported no LMVPAs, and 35.6% of boys and girls reported fewer than one LMVPAs per day. While the majority of children reported obtaining at least some activity daily, a substantial proportion of children in this sample reported fewer than one LMVPA daily, indicating that many children may not be obtaining adequate amounts of physical activity.

Child↗
PubMed1990

Wound infection rates after invasive procedures in HIV-1 seropositive versus HIV-1 seronegative hemophiliacs.

One-hundred and two patients with hemophilia A, hemophilia B, or acquired antibody to factor VIII who had undergone invasive procedures were cross referenced with patients participating in an ongoing prospective natural history study of HIV-1 infection in hemophiliacs. Matching revealed that HIV-1 status was known for 83 patients (83%) who had undergone 169 procedures between July 1979 and April 1988. Invasive procedures were classified as clean in 108 patients (63.9%), clean-contaminated in 45 (26.6%), contaminated in 2 (1.2%), and infected in 14 (8.3%). Wound infection rates by HIV-1 status were as follows (95% confidence intervals): HIV+ 1.4% (0% to 5%), HIV- 0% (0% to 9%), and procedure before testing HIV+ 1.5% (0% to 6%). There were no significant differences between the wound infection rates of HIV-positive and HIV-negative hemophiliacs nor in the wound infection rate among all three subgroups of patients (p greater than 0.5, Fisher's Exact Test). We conclude that surgery in HIV-1-infected patients who have not progressed to AIDS does not entail an increased risk of postoperative wound infections.

HIV Seropositivity↗
PubMed1990

Assessing everyday memory in patients with dysphasia.

An assessment of the current memory status of patients referred for speech therapy is rarely provided, but may be useful in indicating whether there is memory loss in addition to language deficits. Failure to progress during treatment could be due to failure to remember the content of the previous sessions. Most existing memory tests are unsuitable for assessing subjects with dysphasia. For that reason we sought to modify the Rivermead Behavioural Memory Test (RBMT: Wilson, Cockburn & Baddeley, 1985), which was developed to measure the existence and extent of everyday memory problems and which contains both verbally and non-verbally mediated items. The study described here, based on a sample of 176 brain-injured patients, showed that a mild to moderate language deficit did impair performance on the RBMT. The influence was found to be limited to a subset of verbal memory items. A shortened version of the test was developed and shown to be sensitive to memory deficits and insensitive to the effects of dysphasia. Revised norms for this version of the test are provided. These should enable an estimate to be made of the presence and magnitude of memory deficits in dysphasic patients, allowing treatment to be optimized.

Adolescent↗
PubMed1990

A clinical audit of thallium-technetium subtraction parathyroid scans.

Eighty six consecutive thallium-technetium subtraction parathyroid scans performed over a three year period for hypercalcaemia have been evaluated. Twelve had chronic renal failure, 11 had hypercalcaemia due to non-hyperparathyroid causes and in 10 the imaging study was technically inadequate. The remaining 53 technically adequate studies performed for hypercalcaemia clinically thought to be possibly due to hyperparathyroidism have been analysed. Of 20 (38%) positive scans, 13 came to surgery (10 correctly localized parathyroid adenomas, 2 with multiple gland hyperplasia, and 1 papillary carcinoma of the thyroid). Of 33 (62%) negative scans, 9 had surgical exploration on the basis of strong clinical grounds and all had parathyroid adenomas. Multiple biochemical parameters have been assessed in relation to a positive outcome on scan. The adjusted calcium-phosphate product and the ratio of the adjusted calcium-phosphate product to creatinine (Ca x P/Cr) were both significantly lower in the scan positive group (P less than 0.01). The scan positive group had a significantly higher mean level of PTH (P less than 0.001) and lower mean level of phosphate (P less than 0.001). The present experience shows that parathyroid imaging is useful in localizing parathyroid adenomas in 50% of cases (10 out of 19). This figure is at the lower end of the range of previously published results. It is less effective in demonstrating multiple gland hyperplasia. The decision as to whether to undertake surgical exploration when the scan is negative has been based successfully on clinical judgement. We feel that an analysis of this nature is important, as it gives insights into the practical relevance of parathyroid imaging in the context of routine clinical work.

Adenoma↗
PubMed1990

Neuropsychological and psychiatric changes following treatment of ARC patients with zidovudine.

Six homosexual men with a diagnosis of acquired immunodeficiency syndrome (AIDS)-related complex (ARC- CDC IVA/C2) were assessed with a series of neuropsychological and psychiatric tests prior to commencing treatment with zidovudine and again after 5-6 months. Significant improvements were seen in cognitive functioning, mental state and physical health at the second assessment.

AIDS-Related Complex↗
PubMed1990

Outcome-focused counseling program for quality assurance in ambulatory care.

An outcome-focused patient-counseling program designed to enhance quality assurance in ambulatory pharmaceutical care is described. Drugs for which counseling was mandatory because of their potential for association with an adverse outcome were listed. A checklist was created for each drug class to assist pharmacists in providing uniform information and to serve as a follow-up document for quality assurance. The completed forms are used to indicate which patients' charts should be pulled for review against quality assurance criteria for the monitored drug classes; the criteria function as indicators of an adverse outcome. Findings are summarized and presented at the monthly meeting of the departmental quality assurance committee. The program was phased in one drug class at a time. Between October 1988 and January 1989, 18 charts were reviewed for the two drug classes being monitored during that period. There were no patients to whom the indicators of an adverse outcome applied. The outpatient-counseling program helped pharmacists to provide consistent information and to focus on those drugs with the highest potential for adverse outcomes. Incorporating the program into quality assurance activities made it easier to measure patient outcomes.

Ambulatory Care↗
PubMed1989

Identification and characterization of a Mr 50,000 adrenal protein in human hepatocellular carcinoma.

A Mr 50,000 cell surface protein antigen (p50) was identified on a human hepatocellular carcinoma derived cell line (FOCUS) by two monoclonal antibodies (SF 31 and SF 90). This antigen was subsequently shown to be expressed in vivo in human hepatocellular carcinoma. All 18 tumors tested by Western immunoblotting demonstrated high levels of p50 with undetectable amounts observed in the adjacent normal liver counterparts. Further characterization revealed that p50 is a monomeric polypeptide with a neutral pI (6.5-7.2) and appears not to be glycosylated. The cellular localization was determined by direct antibody binding to intact cells, immunoprecipitation of 125I-labeled cell surface proteins, and Western immunoblotting of subcellular fractions. p50 was found on the cell surface as well as in the cytoplasm. In vitro monoclonal antibody binding studies indicate that the protein is expressed in all human malignant cells (n = 34) tested thus far regardless of the embryonic tissue of origin and the degree of differentiation. p50 was present at very low levels in normal tissues with the notable exception of high expression in adrenal glands. The protein is conserved in mammalian evolution since a similar protein was also found in bovine adrenals. The molecular characteristics and the pattern of expression of p50 indicate that this normal adrenal protein is associated with the transformed phenotype.

Adrenal Glands↗