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Biomedical subjects

B Walter

Publications and source records attributed to B Walter.

At least 109 records · Page 6Linked to original sources
PubMed1986

Ultrastructural development of dated plaque in case of denture stomatitis.

Using special acrylic discs glued into the palatal surface of a denture the development of denture plaque in a case of denture stomatitis as well as the relationships between the denture surface, pellicle, plaque and palatal epithelium were studied with transmission electron microscopy after 30 mn, 1 h, 4 h, 8 h, 24 h, 48 h, 9 days and 29 days. A thin pellicle which increased in thickness from 9 hours to 28 days was visible at the surface of the acrylic denture. It appeared that bacteria retained in the palatal epithelial intercellular spaces were the source of the plaque which developed at the surface of the acrylic denture. Initially loosely packed cocci-like and a few rod-shaped Gram positive bacteria appeared in 8 hours samples at the denture-epithelium interface. An important increase in denture plaque thickness was noted between 24 and 48 hours. In 9 and 28 days samples, coccoid and rod-shaped Gram positive and Gram negative bacteria were present and filamentous bacteria began to be apparent. In all the samples studied, Candida was rare.

Acrylic Resins↗
PubMed1985

Aplastic anemia occurring as amegakaryocytic thrombocytopenia with and without an inhibitor of granulopoiesis.

Two patients with aplastic anemia evolving from cellular bone marrows with severely diminished megakaryocytes are reported. During this evolution a plasma inhibitor of in vitro granulocyte-macrophage colony formation was demonstrated associated with non-A, non-B hepatitis in one patient. The second patient had abnormal liver function that corrected after the delivery of a normal newborn but there was persistence of pancytopenia without evidence of a plasma inhibitor.

Adult↗
PubMed1985

Solid-phase analytical elements for clinical analysis.

The technology of dry reagent chemistries is sufficiently advanced so that analytical elements can be developed for almost any clinical analyte. Each element contains all the reagents and separation steps necessary to conduct an analysis. This provides the user with several advantages. The ability to obtain several analyses from small sample volumes has always been recognised as desirable with neonatal and geriatric patients. With dry reagent chemistries, usually 10 microliters of serum or 30 microliters of whole blood are sufficient per analysis. Hence, a profile of 15 tests can be conducted with 150 microliters of serum. The waste of reconstituted but unused reagents is eliminated. This provides more efficient and cost-effective use of reagents. The reagents are stable and require little storage space. In addition, they are extremely convenient to use and require only a small amount of technical skill. This allows for expansion of clinical analyses into such decentralised settings as the hospital ward, physician's office and even the home.

Algorithms↗
PubMed1985

Ultrastructural relationship of denture surfaces, plaque and oral mucosa in denture stomatitis.

Denture plaque has been studied by transmission electron microscopy in glycolmethacrylate-embedded specimens from six patients presenting with typical denture stomatitis. Different types of thin and thick pellicles were found applied to the denture surface. These were in contact with a denture plaque made up predominantly of loosely packed Gram- positive and Gram- negative bacteria. Rounded, rod-shaped and filamentous micro-organisms were all evident. Group of Candida albicans with normal ultrastructure were noted in some limited zones. When surrounded by bacteria, especially by bacteria in corn-cob configurations the Candida showed cytological signs of degeneration. In one case out of six a bacterial penetration of the acrylic denture base was observed, through narrow and straight channels and subsurface cavities filled with micro-organisms. Bacterial penetration was also seen in the most superficial cell layers of the palatal mucous membrane.

Aged↗
PubMed1985

Dry-reagent strips used for determination of theophylline in serum.

A reagent strip for quantifying theophylline in serum or plasma has been developed for use with the Apoenzyme Reactivation Immunoassay System (ARIS) with the Ames Seralyzer reflectance photometer. The test takes 80 s and involves comparison with a two-point calibration line, which can validly be stored in the instrument for two weeks. Results for theophylline in clinical serum samples correlate well (r greater than 0.98) with results by liquid chromatography, fluoroimmunoassay, and enzyme immunoassay procedures. The within-run CV for four concentrations of controls ranged from 3.5 to 6%; the between-run CVs ranged from 3 to 5%. This assay for use in therapeutic drug monitoring is convenient, rapid, and simple, and thus is appropriate for use in emergency rooms, physician's offices, and small laboratories.

Chromatography, Liquid↗
PubMed1983

[Conservative and surgical treatment of radius fractures loco classico].

More and more distal fractures of the radius are treated surgically, thereby decreasing the treatment time and quickly restoring use of the arm. The rarely seen compound fractures of the radius and those involving circulation problems or loss of feeling in the hand will be treated as emergency cases. The most frequently used treatment is osteosynthesis using pins. In cases of Smith fracture, with young patients or corrective surgery the preferred method is osteosynthesis using metal plates. If, because of extensive fragmentation, a non-operative treatment would certainly lead to dislocation, or a secondary dislocation due to a non-operative treatment has already taken place, the surgical treatment should be discussed. Dislocated intraarticular fractures are increasingly being treated immediately surgically.

Adolescent↗
PubMed1981

Posterior meatal wall reconstruction in tympanoplasty.

An operative method is described which has been employed in 41 cases of severe chronic otitis media. The posterior meatal wall was removed and reconstructed during the same operation, following cleansing of the middle-ear cavity and ossiculoplasty. Either autograft bone (previously removed from the meatal wall) or homograft cartilage was employed for the reconstruction. All the ears were found to be dry at the follow-up examination after an average of 28 months, but 18 per cent had recurrence of the cholesteatoma. A SRT improvement of 5-30 db, was observed in 78 per cent, and in 21 per cent a deterioration of between 0 and 10 db. The method provides a good field of vision of the middle-ear cavity and in no cases was rejection of the re-implanted material observed.

Adolescent↗
PubMed1981

Homogeneous fluorescent immunoassay with dry reagents.

We described an assay for theophylline in serum in which the reagents for the substrate-labeled fluorescent immunoassay procedure are dry reagents in a paper matrix. This assay format is both rapid and convenient. In one such paper strip assay, the competing drug conjugate is present in the diluting buffer (dilution-into-conjugate strip assay); in another, all necessary assay reagents are contained on the strip (integral strip assay). The latter strip can be prepared by using appropriate solvents in a "two-dip" procedure, which prevents premature reaction of the assay constituents. The dilution-into-conjugates strip assays yield results that compare well with those by the corresponding solution assay (r = 0.975, standard error of estimate = 1.67 mg/L) and by "high-performance" liquid chromatography (r = 0.980, standard error of estimate = 1.49 mg/L). The integral strip results were compared with those by an enzyme immunoassay for theophylline (r = 0.990, standard error of estimate = 2.07 mg/L). Both strip assays require only a single dilution step before analysis.

Humans↗
PubMed1980

Time-space patterns of second-wave Irish immigration into British towns.

"A time-space model, based on Hagerstrand's (1969) methodology, is used to illustrate the diffusion of a migration-stream into new areas at destination and the associated changes in community characteristics at different stages in the settlement sequence." Published regional census data and interviews with Irish-born residents of England provide data on Irish migration into and within Britain since the 1930s

Demography↗
PubMed1979

Immunochemical similarities between subunits of acetylcholine receptors from Torpedo, Electrophorus, and mammalian muscle.

Polypeptide chains composing acetylcholine receptors from the electric organs of Torpedo californica and Electrophorus electricus were purified and labeled with 125I. Immunochemical studies with these labeled chains showed that receptor from Electrophorus is composed of three chains corresponding to the alpha, beta, and gamma chains of receptor from Torpedo but lacks a chain corresponding to the delta chain of Torpedo. Experiments suggest that receptor from mammalian muscle contains four groups of antigenic determinants corresponding to all four of the Torpedo chains. Binding of 125I-labeled chains was measured by quantitative immune precipitation and electrophoresis. Antisera to the following immunogens were used: denatured alpha, beta, gamma, and delta chains of Torpedo receptor, native receptor from Torpedo and Electrophorus electric organs and from rat and fetal calf muscle, and human muscle receptor (from autoantisera of patients with myasthenia gravis). The four chains of Torpedo receptor were immunologically distinct from one another and from higher molecular weight chains found in electric organ membranes. Antibodies to these chains reacted very efficiently with native Torpedo receptor, but the reverse was not true. Antibodies to native receptor from Torpedo and Electrophorus reacted slightly with each of the chains of the corresponding receptor. However, cross-reaction between chains and antibodies to any native receptor was most obviuos with the alpha chain of Torpedo or the corresponding alpha' chain of Electrophorus. Antiserum to alpha chains exhibited higher titer aginst receptor from denervated rat muscle. Antibodies from myasthenia gravis patients did not cross-react detectably with 125I-labeled chains from electric organ receptors. Most interspecies cross-reaction occurred at conformationally dependent determinants whose subunit localization could not be determined by reaction with the denatured chains.

Animals↗
PubMed1979

[Thrombolysis with Urokinase after anaphylaxis caused by streptokinase treatment (author's transl)].

Deep femoral and pelvic phlebothrombosis represents a safe indication for thrombolytic therapy, despite the fact that additionally considered surgical measures have increasingly been brought up for discussion very recently. While numerous studies are being considered for treatment with streptokinase, only sporadically does one hear of reports about therapy using Urokinase. In the case of a deep pelvo-femoral phlebothrombosis we decided to carry out thrombolytic treatment using Urokinase, the originally planned streptokinase treatment having had to be terminated owing to an allergic reaction.

Adult↗
PubMed1979

[The diagnosis of organic brain damage with common psychodiagnostic tests (author's transl)].

WIP, Hopper-VOT, Benton-Test d2-Test, and the KVT were used to examine 124 brain-damaged people with sufficiently secured localized wounds on the brain (48 frontal, 30 temperoral, 29 parietal and 17 occipital lobe damaged patients). The results showed considerable differences depending on the localisation of the injury. The differences between the frontal lobe damaged and the parieto-occipital damaged patients were significant. A normal score in one or more tests does not exclude a frontal lobe damage.

Brain Damage, Chronic↗