Prolactin synthesis in primates.
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Biomedical subjects
Publications and source records attributed to B W Low.
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Bovine proinsulin has been crystallized under a variety of conditions at both neutral and acid pH. Microtechniques were employed with sample weights of about 200 mug and volumes of 5-20 mul. The crystalline preparations all differ from each other morphologically.X-ray photographs of one form, tetragonal bipyramids grown at pH 3 with added ammonium sulphate solution, established the space group P4(1)2(1)2 (or its enantiomorph P4(3)2(1)2). The cell dimensions are a = 50.8 +/- 0.2 A, c = 148.0 +/- 0.4 A. The asymmetric unit in this form is a dimer of proinsulin which is also the dominant species in solution at this pH.
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X-ray studies of crystalline deamino-oxytocin (1-beta-mercaptopropionic acid-oxytocin) have established the existence of both wet and dry forms. A remarkable degree of similarity exists between the two forms even though there are changes in cell dimensions and space group on drying. The dry form of 1-gamma-mercaptobutyric acid-oxytocin is very similar to that of deamino-oxytocin.
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