Comparative studies of the diagnostic value of three express methods for detection of tick-borne encephalitis virus in ticks (fluorescent antibody technique (FAT) enzyme immunoassay (EIA), and molecular hybridization of nucleic acids) and the traditional method (bioassay in white mice) showed all the three express methods to be rapid, specific, sensitive, and useful for large-scale epidemiological surveys. Notable was the high effectiveness of the method of nucleic acids hybridization which was not inferior to bioassays in suckling mice and exceeded FAT and EIA. The results of the latter seem to be affected by antigenic variations among tick-borne encephalitis virus strains.

Animals↗

PubMedDate not supplied

[Epidemiological surveillance of natural foci of tick-borne encephalitis in the recreational area of a large city (the methodology and results].

Explore the source record for details and available documents.

Animals↗

PubMedDate not supplied

[Detection of the etiologic agent of hemorrhagic fever with a renal syndrome in the lungs of wild rodents].

The results of the studies on wild rodents trapped in the areas of the Leningrad region visits to which were associated with human cases of hemorrhagic fever with renal syndrome (HFRS) are presented. In one of these areas 30% of bank voles were found to be infected. Specific fluorescence of high intensity was found in ctyostat sections of lungs from bank voles treated by the indirect fluorescent antibody procedure. Fluorescent granules, their clumps and large conglomerates were localized in the cytoplasm of alveolar epithelium cells. The antigen-containing cells formed foci within which 70% or more elements showed fluorescence or were found diffusely throughout the section (dozens of infected cells in each field of vision). The antigen-containing lung sections were used for the indirect immunofluorescence test with sera from the subjects clinically diagnosed as having HFRS. Positive results in titers ranging from 1 : 10 to 1 : 1280 were obtained in 6 out of 7 subjects examined. Examinations of paired sera from one of them revealed an over 100-fold rise in titers during the 3rd-7th week after the onset of the disease.

Animal Population Groups↗

PubMedDate not supplied

[Etiology of Karelia fever--a new arbovirus infection].

In August-September 1981, in some areas of Karelia (on the border of northern and middle taiga) there occurred diseases with signs of fever, rash, and arthralgia developing in some patients into a chronic condition. Examinations of paired sera from patients with the acute disease and from convalescents using a set of Toga- and Bunya-viruses showed the etiology of the disease to be associated with a virus from the Togaviridae family, genus Alphavirus, of the antigenic Sindbis complex.

Adolescent↗