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Biomedical subjects

B V Jones

Publications and source records attributed to B V Jones.

34 records · Page 2Linked to original sources
PubMed1989

Investigation of bovine gut associated lymphoid tissue (GALT) using monoclonal antibodies against bovine lymphocytes.

Gut associated lymphoid tissue of the small and large intestine of calves and cows has been compared morphologically and quantitatively using monoclonal antibodies to bovine lymphocytes. B cells were significantly decreased in the ileum of the cow compared to the calf. Significantly increased numbers of T cells were present in cell suspensions of all lymphoid areas of the cow compared to the calf. T lymphocyte subsets were quantified into cryostat sections of lymphoid tissues expressing BoT4, and BoT8 antigens demonstrated increased numbers in follicular and dome areas of the discrete Peyer's patches of the small and large intestine of the cow. BoT4+, BoT8+, and the non-BoT4/BoT8+ T cell subsets were increased in the mucosa of the cow as compared to the calf. Similarities in structure and lymphocyte composition of the discrete Peyer's patches of the small intestine, cecum and colon and isolated single follicles in the large intestine suggest similar functional properties.

Analysis of Variance↗
PubMed1988

Two monoclonal antibodies (CC17, CC29) recognizing an antigen (Bo5) on bovine T lymphocytes, analogous to human CD5.

Two new monoclonal antibodies (CC17 and CC29) raised against bovine thymocytes are described. The antibodies, both of which were IgG1, recognize a molecule of approximately 67,000 molecular weight on bovine T cells. They react T cells in peripheral blood, the lymph node paracortex and the periateriolar lymphoid sheath in the spleen. Both the cortex and medulla of the thymus are stained but the medulla reacts more intensely. They do not stain B cells in peripheral blood, the ileal Peyer's patch, the cortex or the primary follicles in lymph nodes. No activity was found on cells outside the lymphoid system, i.e. monocytes, alveolar macrophages or endothelial and epithelial tissue. The antigen recognized is considered to be the bovine homologue of CD5 (T1) in humans and Lyt1 in mice. The mAbs appear to be particularly useful for detecting cells in the peripheral blood of young calves which are of the T cell lineage but do not express BoT2 or the mature pan T cell antigen recognized by mAb IL-A27 and may thus allow identification of a population of bovine lymphocytes previously described as null cells.

Animals↗
PubMed1988

Production and characterization of bovine monoclonal antibodies to respiratory syncytial virus.

Six interspecific hybridomas (heterohybridomas) secreting bovine monoclonal antibodies (MAbs) against respiratory syncytial (RS) virus were produced. Four of the heterohybridomas were formed using the mouse myeloma cell line NS1 as the fusion partner, one using NS0, and the remaining heterohybridoma was formed using a bovine X murine hybridoma as the fusion partner. Five heterohybridomas secreted bovine IgG1 and one secreted IgG2. All six MAbs recognized human subtype A and B viruses as well as bovine RS virus. They were specific for the fusion glycoprotein and reacted with a 140K dimer and a 70K monomer in a Western blot of native antigen; three also bound to the 46K F1 component and its 22K cleavage product in a blot of reduced antigen. Two of these MAbs neutralized RS virus infectivity, inhibited virus-induced fusion, lysed RS virus-infected cells in the presence of complement and protected mice against RS virus challenge.

Animals↗
PubMed1980

Pyocin inhibition of Neisseria gonorrhoeae: mechanism of action.

Purified R-type pyocins (611 131) from Pseudomonas aeruginosa PA103 exhibited bactericidal activity against Neisseria gonorrhoeae. Killing of gonococci was a single-hit process requiring as few as 1 pyocin per colony-forming unit. Deoxyriboinucleic acid, ribonucleic acid, protein, and lipid syntheses were rapidly and completely inhibited. Oxygen uptake was also inhibited, but occurred after the inhibition of macromolecular synthesis. The cell lysis which occurred after pyocin inhibition of gonococcal growth was the result of endogenous gonococcal autolysin activity.

Adenine↗
PubMedDate not supplied

Diagnosis of posttraumatic pericardial tamponade by plain film and computed tomography and control of bleeding by embolotherapy of the left inferior phrenic artery.

A 21-year-old male developed pericardial tamponade in a delayed fashion after blunt chest trauma. Tamponade was not suspected clinically and was first seen on computed tomography of the abdomen. Plain films of the chest revealed distortion of the path of a Swan-Ganz catheter due to the ventricular compression. After drainage, left inferior phrenic artery embolization was performed to treat the persistent hemothorax.

Adult↗
PubMedDate not supplied

Policy on welfare.

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Animal Welfare↗
PubMedDate not supplied

Clinico-chemical changes in goats given carbon tetrachloride.

Six healthy castrated male goats were each given 1.0, 1.25 or 1.5 ml of carbon tetrachloride per 10 kg BW by stomach tube (2 animals at each dose level). Definite signs of dose dependant liver damage were seen, starting about 4 hours after administration of the drug. The clinico-chemical changes included increased total and conjugated plasma bilirubin, decreased blood clotting activity, and increased activities of P-ASAT and P-GT. Further experiments are needed in animals afflicted with liverflukes to establish the lowest possible anthelmintic dose of carbon tetrachloride.

Animals↗