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Biomedical subjects

B T Philipson

Publications and source records attributed to B T Philipson.

22 records · Page 2Linked to original sources
PubMed1977

Formation of after-cataract by regeneration of human and rabbit lens epithelium in tissue culture.

Regeneration of lens epithelium on the lens capsule was studied in tissue culture. The entire capsule with attached epithelium was taken from rabbit lenses and from human lenses with cataract. Generally, the epithelium grew in a monolayer but multilayered masses of cells were also seen. Most lens fibers degenerated during the first days and formed spherical membrane enclosed vesicles containing cytoplasm but no nuclei. The lens fiber remnants, together with regenerating epithelium, created structures that were similar in many ways to the clinical appearance of after-cataract.

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PubMedDate not supplied

Intraocular levels of cefuroxime in inflamed rabbit eyes.

The pharmacokinetics of cefuroxime was studied in the inflamed rabbit eye employing subconjunctival, intravitreal and combined intravitreal-intravenous routes of administration to study the intraocular levels and the duration of minimum inhibitory concentrations (MIC) of the antibiotic in the vitreous and in the aqueous. A standard inoculum of viable S. aureus was injected into the vitreous of 36 pigmented rabbits to establish experimental endophthalmitis. A biological method was used for the antibiotic assay. Penetration of systemically and subconjunctivally administered-cefuroxime into the inflamed vitreous was poor. Intraocular inflammation increased the clearance of the intravitreally injected cefuroxime. A dose of 75 mg subconjunctivally produced levels in the aqueous far exceeding MIC for over six hours. Penetration of intravitreally injected cefuroxime into the aqueous was poor, inconsistent and short lasting. Following a single intravitreal injection of 1000 micrograms cefuroxime, levels exceeding the MIC for common ocular pathogens persisted in the vitreous for at least 24 hours but supplementation with intravenous cefuroxime neither increased the intraocular levels nor delayed the clearance of the intravitreally injected antibiotic.

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PubMedDate not supplied

Lactate levels in the rabbit cornea and aqueous humor subsequent to non-gas permeable contact lens wear.

The validity of using the aqueous humor lactate concentration as an indicator of corneal lactate production was tested. For this purpose, ten rabbits wore non-gas permeable contact lenses on one eye for 12 hours. Aqueous humor and corneal extracts were assayed for lactate by means of an enzymatic technique. The contact lens wear caused a limited increment in the corneal lactate concentration, but no change was recorded in the aqueous humor. We therefore conclude that the corneal lactate production has a negligible effect on the lactate concentration in aqueous humor. However, significant individual variations in lactate levels were recorded in the present study, as in earlier ones. Insufficient specificity in the currently used techniques for lactic acid concentration determination may in part explain this.

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