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Biomedical subjects

B Stojan

Publications and source records attributed to B Stojan.

31 records · Page 2Linked to original sources
PubMedDate not supplied

[The potentiating effects of azathioprine and azapropazone on the enzyme activity in experimental inflammation].

The influence of prednisolone, azathioprine, and azapropazone on the activity of collagen peptidase, acid phosphatase (complete), and the lactate dehydrogenase of granulation tissue was studied using a model of proliferative inflammation i.e. the cotton-granuloma in rats. The authors observed significant decreases in the activities of the collagen peptidase and acid phosphatase during treatment with prednisone and under combined treatment with azathioprine and azapropazone. In contrast, administration of either azathioprine or azapropazone by itself caused no changes in these enzyme activities as compared with a control group. These studies suggest that the last mentioned drugs potentiate each other in their inhibiting effect upon these enzyme activities. Whether or not these findings are of importance in the clinical treatment of chronic bacterial inflammations must be studied in further trials.

Acid Phosphatase↗
PubMedDate not supplied

[Two-phase course of IgM and rheumatoid factors during D-penicillamine therapy].

16 patients with definite and classical rheumatoid arthritis (A.R.A. criteria) were under regular control for 210 days. 11 of them were treated with D-penicillamine and 5 with gold. They were compared to a group of 34 healthy people. In addition to clinical observations the following investigations were carried out at intervals of 20 to 30 days: Latex-fixation test and Waaler-Rose, IgM, IgA, IgG, C3, C4, C3-proactivation, ESR, coeruloplasmin, iron, complete blood picture, gamma-GT, SGOT, SGPT, alk. phosphatase, creatinine, urea and full urinalysis. Furthermore antinuclear factors (ANF) and C-3 activating ANF were determined by indirect immunofluorescence. The following observations were made: 1. The serum level of immuno-globulins changed in a two-phase fashion during D-penicillamine treatment. Initially IgM decreased significantly until the 60th to 80th day. During the 80th to 210th day it tended to increase. Rheumatoid factors changed in a similar way. 2. There was a significant correlation between the IgM serum level and the average titer of the Latex-fixation and Waaler-Rose tests. 3. The other parameters did not change significantly. 4. In one case the ANF became positive and showed a tendency to increase in titre. With higher titres complement activation was demonstrated. The treatment was discontinued. Thereafter the ANF titres and complement binding decreased gradually.

Antibodies, Antinuclear↗
PubMedDate not supplied

[A benign form of rheumatoid arthritis, associated with low IgG, IgM and IgA and C3-proactivator concentrations in the serum].

In 31 case studies, 29 patients diagnosed with definite rheumatoid arthritis and 2 patients with probable rheumatoid arthritis were investigated. The duration of the disease averaged 8 years with 3 years being the minimum. These patients received either no basic therapy or had not been treated six months previous to the investigation. Patients with low levels of immunoglobulins were shown to have significantly better functional capacity (Steinbrocker) and significantly less synovitis, erosions and joint destructions despite longer duration of the disease than the control group with higher levels of immunoglobulins and C3-proactivator. All patients with this benign development of rheumatoid arthritis had an IgG-level below 1000 mg/100 ml (mean 767 mg/100 ml +/- 166) and low levels of IgA (mean 194 mg/100 ml +/- 73), IgM (mean 111 mg/100 ml +/- 53) and C3-proactivator (mean 23 mg/100 ml +/-6). 50% of these patients had positive rheumatoid factors.

Arthritis, Rheumatoid↗
PubMedDate not supplied

[Activated C3 in the synovial fluid of patients with rheumatoid arthritis and arthrosis (correlations with C3, C4 and C3 proactivator/modification caused by storage)].

Cleavage products of C3 in synovial fluid were determined by the method of 2-dimensional electrophoresis (Laurell). Statistically significant differences were observed in rheumatoid synovial fluid compared with the osteoarthrosis group. Storage is of crucial importance for the in vitro activation of C3. In fresh synovial fluid there was a significant positive correlation between breakdown products of C3 and C3, C4 and C3-proactivator. After storage (even in -70 degrees C) this positive correlation in the rheumatoid synovial fluid changes into a negative one. This phenomenon is not observed in synovial fluid from patients with osteoarthrosis. There is evidence that in the rheumatoid synovial fluid C3-activating fractions in 40S, 19S and 2,5S are responsible for the generation of C3 cleavage products.

Arthritis, Rheumatoid↗
PubMedDate not supplied

[Complement (C3)-activating material in synovial fluid from patients with rheumatoid arthritis].

Synovial fluid from patients with the following diagnoses was investigated: - rheumatoid arthritis (RA) - rheumatoid arthritis with benign development (BRA) - osteoarthrosis (A) The sediment was isolated by ultracentrifugation, its C3 cleaving activity measured by 2-dimensional electrophoresis and its concentration if IgG, IgM, IgA, C3, C4 and C3-proactivator by immunoprecipitation. There is evidence that there is more C3 cleaving activity in RA than in osteoarthrosis. Activity levels of BRA were between those of osteoarthrosis and RA. Although there was no significant difference in the IgG-, IgM- or IgA-level of the 3 mentioned diagnoses after 21 minutes of centrifugation (133 000 g), it was possible to demonstrate more C3 breakdown products in RA than in osteoarthrosis and BRA. Very big molecules seem to be responsible for the generation of C3 cleaving products. These observations are not only of theoretical interest but seem also to be useful diagnostically.

Arthritis, Rheumatoid↗
PubMedDate not supplied

[D-penicillamine and wound healing in patients with rheumatoid arthritis].

The inhibiting effect of D-penicillamine on the collagen metabolism in animal experiments and in some published clinical observations appear to confirm the assumption that this drug might retard the wound healing after surgery. Therefore the authors studied wound healing following 107 orthopedic operations in 49 RA patients: 36 operations on patients without any basic treatment, 48 during D-PA, 18 during gold, 4 during azathioprine and 1 during Resochin. Retarded wound healing (up to 4 weeks after the date of the operation) was observed 6 times: in 2 out of 36 operations (5.5%) in those without basic treatment, in one out of 48 (2.1%) during D-PA (600 mg/day), in 3 out of 18 (16.6%) during gold therapy. Operative re-interventions because of disturbances in the wound healing or infections were not needed. Based on these observations and the fact that authors who have observed an inhibition of the collagen synthesis have used an uncomparibly higher dosage per kilogram of body weight, the authors of this report are so far of the opinion that the dosage they are using (300-600 mg/day) does not significantly influence the speed of wound healing. Thus the basic treatment must not be interrupted before or after an operation.

Adult↗
PubMedDate not supplied

[Elevated C3-proactivator serum levels in patients with chronic polyarthritis].

The C3-Proactivator (C3-PA) and the C3 and C4 complements were determined by radio-immuno diffusion in the serum of 72 patients with definite or classical rheumatoid arthritis, 51 patients with osteoarthrosis and 42 healthy subjects. C3-PA and C4 levles were significantly higher in the serum of patients with rheumatoid arthritis than in the control group (C3-PA in 72%, C4 in 37% of the RA patients). There was also a significant difference between seropositive und seronegative cases. Elevation of both components was more frequently found in patients with seronegative rheumatoid arthritis than in the seropositive cases. However, the C3-PA serum level is not correlated with the ESR. Also an elevation of the C3-PA serum level is not specific for rheumatoid arthritis, it is also found in other inflammatory diseases as well as after surgery.

Arthritis, Rheumatoid↗