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Biomedical subjects

B Singh

Publications and source records attributed to B Singh.

At least 1,045 records · Page 58Linked to original sources
PubMedDate not supplied

Renal dysplasia with multisystem malformation--a study of 9 cases.

Nine cases of renal dysplasia encountered in a review of necropsy material are described. All these cases had associated major and minor malformations of other systems. Three out of four cases identifying with 'prune belly' syndrome had lower urinary tract obstruction. Three cases fulfilled the criteria of Meckel's syndrome. Three of these six cases, in addition, showed overlapping features with other syndromes. Three cases of unilateral dysplasia with contralateral agenesis had associated anomalies not conforming to any definite combination. We suggest that the association of malformations of developmentally unrelated systems may be random, not sharing any feature except being incompatible with life.

Abnormalities, Multiple↗
PubMedDate not supplied

Combined action of lead and lithium on essential and nonessential elements in rat blood.

The effects of lead and lithium ingestion, separately and in combination, on the levels of K, Fe, Cu, Zn, Br, Rb, and As in rat blood were studied by the Energy Dispersive X-ray Fluorescence technique. Two different doses of lead acetate, i.e., 50 and 100 mg/Kg body wt (low and high doses), were administered orally to rats, daily, for 1 and 4 months (short and long terms), whereas lithium in the form of lithium carbonate was given to rats in food (1.1 g/kg diet) for 1 and 4 mo separately, and also to rats receiving lower and higher doses of lead. K levels were found to be depressed significantly with lead treatment, whereas Fe contents were enhanced marginally after 1 mo of treatment when only the higher dose of lead was given. As, Br, and Rb contents were found to be elevated following lead treatment for short and long terms at both the dose levels. However, Cu contents were lowered, whereas Zn contents were raised only after long term treatment with lead. The Fe, Cu, As, and Br contents remained unaltered, whereas K, Rb, and Zn contents were reduced significantly when lithium was administered for short term. Moreover, Cu and Fe levels were also found to be reduced and Br contents were enhanced only after long term treatment. During the combined treatment with lead and lithium for short and long terms, the levels of K, As, and Rb were observed to decrease, whereas Fe contents were enhanced when estimated for both doses of lead. On the contrary, Cu levels were lowered only with the higher dose of lead acetate when given in combination with lithium for 1 and 4 mo. Br contents were only effectively decreased after 4 mo of treatment.

Animals↗
PubMedDate not supplied

Pulse polio immunization in Delhi--1995-96: a survey.

A coverage evaluation survey of the campaign for mass Pulse Polio Immunization (PPI) on 9th December, 1995 and 20th January, 1996 in Delhi was carried out using the modified cluster sampling technique and a pre-structured proforma. Six-hundred-and-nine children of under-3 age group were covered in the survey. Overall coverage for both the doses was found to be 77%. While coverage for 9th December dose was found to be 80%, it rose to 90.2% on 20th January, 1996. Coverage levels for male and female children were similar. Parental literacy was seen as a definite factor, positively affecting the coverage levels. Proportion of not covered under PPI was significantly higher in the 0-6 months age group. Television and health workers were found to be the main sources of awareness about PPI.

Child, Preschool↗
PubMedDate not supplied

Immune responses to self peptides naturally presented by murine class II major histocompatibility complex molecules.

Peptides eluted from murine Major Histocompatibility Complex (MHC) class II molecules are predominantly fragments of self proteins, which include apolipoprotein E, cystatin-c, transferrin receptor, MHC class II and Ii chains. These naturally processed self peptides are expected to be presented during ontogeny. Therefore, immune responses to these peptides in syngeneic hosts may be under physiological control so as to modulate auto-reactivity. As would be expected from our current understanding, T cells reactive to such antigens should be deleted or clonally anergized. To explore this possibility, we investigated the immunogenicity of a number of these self peptides in mice that express MHC class II, from which these peptides were eluted. T cell and antibody responses were measured following immunization of mice with the appropriate peptide. Surprisingly, many of these peptides were highly immunogenic in normal mice. T cells reactive to these self peptides are restricted by syngeneic MHC class II and were blocked by alpha CD4 antibodies. T cells primed with the native protein in vivo could be challenged with the appropriate self peptide in vitro. Some of the self epitopes induce Th1 cells as indicated by IFN-gamma but not IL-4 production and others induce Th2 cells. Antipeptide antibodies were detected only at higher doses of antigen. Our results suggest that T cells specific for many of the naturally processed self peptides are not deleted but tolerance to these peptides is still maintained in vivo. Presumably the high-affinity self-reactive T cells are deleted in the thymus and the low-affinity self peptide reactive T cells remain unresponsive to antigen challenge in vitro. Upon antigen priming in vivo, many of these self-reactive T cells become activated and readily respond to antigen challenge in vitro. These results point to the physiological control of the maintenance of tolerance to naturally processed self peptides.

Amino Acid Sequence↗
PubMedDate not supplied

Post-partum psychoses and the dexamethasone suppression test.

The hypothesis that post-partum psychoses are predominantly mixed affective disorders was tested by administering the dexamethasone suppression test (DST) to seven puerperal psychotics, six puerperal depressives and comparison groups of non-puerperal psychotic and depressed women. The hypothesis received support from the finding that two-third of the puerperal patients had positive DSTs versus one-third of similar non-puerperal patients. An 80% rate of positive DSTs in 19 normal women 5 days post-partum, however, suggested this was an invalid interpretation of this finding, particularly as the majority of these tests returned to normal when repeated several weeks later.

Brief Psychiatric Rating Scale↗
PubMedDate not supplied

Substernal goiter: a clinical review.

Thyroid disease is a very common problem, but indications for surgery are few. We have seen a large number of patients with multinodular goiter. The main indications for surgery in thyroid disease include fear of malignancy, tracheo-esophageal compression, and cosmetic reasons. Tracheo-esophageal compression is most commonly noted in patients with mediastinal goiters. Substernal goiter is defined as those situations in which at least 50% of the gland is in the mediastinal location. Although its incidence has decreased, it remains prevalent in almost every country in the world today. SSG is best diagnosed by a thorough history and physical examination, complemented by airway films, fiberoptic laryngoscopy, and computerized tomography. The most common presenting symptoms are those produced secondary to compression effects. SSG show a poor response to medical treatment. Moreover, given their propensity to cause acute airway symptoms, surgical treatment should be considered in most cases. Extirpation of the gland is best performed through a collar incision, with the addition of a median sternotomy in select few and difficult cases. Median sternotomy is necessary in only 1% to 2% of cases. Operative mortality is negligible, and the incidence of complication is minimized by following strict surgical principles.

Diagnostic Imaging↗
PubMedDate not supplied

The impact of milk-derived unconditioned and conditioned negative feedback on the microstructure of ingestive behavior.

We describe at the microstructural level the impact of unconditioned and conditioned negative feedback on the licking behavior of the rat. Six groups of rats were trained to ingest one of six different dilutions of sweetened milk (0.5:1, 1:1, 2:1, 4;1, 8:1, and 16:1, water to milk ratios) under real feeding conditions until intake was stable. Then they were given five sham-feeding tests with the same solution. We compared the size of the clusters (SC) and their number (NC) during the 15-min period when intake rate was declining in the real-feeding test with SC and NC during the corresponding 15-min period in the following sham-feeding test. Intake increased significantly over the five sham-feeding tests with the two highest concentrations, indicating the extinction of conditioned negative feedback. With these two solutions, we compared the microstructure of licking behavior in the first with that in the fifth sham-feeding test to determine if conditioned negative feedback affected SC or NC or both. The effect of both unconditioned and conditioned negative feedback on licking behavior was to decrease the number of clusters without affecting their size. We conclude that negative feedback derived from the accumulation of milk in the gastrointestinal tract decreases the probability of initiating a bout of licking during a pause. It has no effect on the ability to continue a bout of licking once it has begun.

Animals↗
PubMedDate not supplied

Detection of malaria in Malaysia by nested polymerase chain reaction amplification of dried blood spots on filter papers.

A modified nested polymerase chain reaction (PCR) method for detection of Plasmodium falciparum, P. vivax and P. malariae was combined with a simple blood collection and deoxyribonucleic acid (DNA) extraction method and evaluated in Malaysia. Finger-prick blood samples from 46 hospital patients and 120 individuals living in malaria endemic areas were spotted on filter papers and dried. The simple Chelex method was used to prepare DNA templates for the nested PCR assay. Higher malaria prevalence rates for both clinical (78.2%) and field samples (30.8%) were obtained with the nested PCR method than by microscopy (76.1% and 27.5%, respectively). Nested PCR was more sensitive than microscopy in detecting mixed P. falciparum and P. vivax infections, detected 5 more malaria samples than microscopy on the first round of microscopical examination, and detected malaria in 3 microscopically negative samples. Nested PCR failed to detect parasite DNA in 2 microscopically positive samples, an overall sensitivity of 97.4% compared to microscopy. The nested PCR method, when coupled with simple dried blood spot sampling, is a useful tool for collecting accurate malaria epidemiological data, particularly in remote regions of the world.

DNA, Protozoan↗
PubMedDate not supplied

Non-radioisotopic glucose turnover in children with falciparum malaria and enteric fever.

To determine whether glucose turnover is increased in acute falciparum malaria compared to enteric fever in children, steady-state 6,6-D2-glucose turnover was measured in 9 Malaysian children with uncomplicated malaria (6 males and 3 females; median age 10 years, body weight 22 kg) and in 12 with uncomplicated enteric fever (8 males and 4 females; median age 10 years, body weight 24 kg) in acute illness, after quinine (5 malaria patients) and in convalescence. Baseline plasma glucose concentrations in malaria and enteric fever were similar (all values are medians [ranges in brackets]) 5.6 [3.2-11.3] vs. 5.5 [4.2-8.0] mmol/L), as were serum insulin levels (5.6 [0.4-26.5] vs. 6.8 [1.1-22.5] milliunits/L; P > 0.4). Glucose turnover in the malaria patients was higher than in patients with enteric fever (6.27 [2.71-6.87] vs. 5.20 [4.50-6.08] mg/kg.min; P = 0.02) and in convalescence (4.74 [3.35-6.79] mg/kg.min; P = 0.05 vs. acute malaria study), and fell after quinine together with a rise in serum insulin (P = 0.03). Basal plasma lactate concentrations were higher in enteric fever than in malaria (3.4 [1.8-6.4] vs. 0.8 [0.3-3.8] mmol/L; P < 0.0001) and correlated inversely with glucose turnover in this group (rs = -0.60; n = 12; P = 0.02). These data suggest that glucose turnover is 20% greater in malaria than in enteric fever. This might reflect increased non-insulin-mediated glucose uptake in falciparum malaria and/or impaired gluconeogenesis in enteric fever, and may have implications for metabolic complications and their clinical management in both infections.

Adolescent↗
PubMedDate not supplied

Head and neck manifestations of non-Hodgkin's lymphoma in human immunodeficiency virus-infected patients.

PURPOSE: Non-Hodgkin's lymphoma is the 2nd most common malignancy in human immunodeficiency virus (HIV)-infected patients. However, limited information regarding head and neck manifestations of non-Hodgkin's lymphoma is present in the literature. The aim of this article is to describe the head and neck manifestations of non-Hodgkin's lymphoma in HIV-infected patients and compare it with that seen in noninfected patients. PATIENTS AND METHODS: A case-control study was performed including 124 patients with non-Hodgkin's lymphoma presenting over a 5.5-year period to tertiary care center in a metropolitan location. RESULTS: Overall, the anatomic distribution of non-Hodgkin's lymphoma is not altered in the presence of HIV infection with the head and neck region (63%) most often involved overall. However, within the head and neck region, extralymphatic disease is significantly more common in HIV-infected patients (59%) than noninfected patients (33%; P = .001). Central nervous system (CNS) involvement accounts for 41% of head and neck non-Hodgkin's lymphoma in HIV-infected patients, in contrast to only 12% of noninfected patients. High-grade lymphoma (68%) are more common than intermediate (30%) or low-grade disease (2%) in the HIV-infected population, whereas low (24%) and intermediate (60%) grades are more common than high-grade lymphoma (16%) in noninfected patients (P < .001). The large cell immunoblastic type (48%) is the most common subtype in HIV-infected patients, whereas diffuse large-cell type (32%) was most common in HIV-negative patients (P < .05). Survival is significantly poor for HIV-infected patients (P < .05). The impact of HIV infection on survival remain significant even after controlling for the effects of confounding factors. CONCLUSIONS: Head and neck involvement with non-Hodgkin's lymphoma occurs in a significant number of HIV-infected patients. Our data show that the distribution and course of non-Hodgkin's lymphoma is unique in HIV-infected patients. A high level of suspicion for non-Hodgkin's lymphoma is required in all cases of head and neck lesions in patients with HIV infection to facilitate management.

Adult↗