Biomedical subjects
B Sepúlveda
Publications and source records attributed to B Sepúlveda.
[Truth and error in medicine].
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[Stages of medical education in Mexico].
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[Advances in the consolidation of the national health system].
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[New orientations in medicine: cellular receptors in therapeutics. I. Introduction].
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[8th symposium on amebiasis. Opening address].
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[Induction of immunity to amebiasis in subhuman primates by lysosomal antigen of Entamoeba histolytica. I. Introduction].
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[Induction of immunity to amebiasis in subhuman primates by lysosomal antigen of Entamoeba histolytica. III. Counterimmunoelectrophoresis and indirect hemagglutination reactions in monkey serum].
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[Induction of immunity to amebiasis in subhuman primates by lysosomal antigen of Entamoeba histolytica. IV. Cytopathogenic effect of monkey serum on trophozoites of Entamoeba histolytica].
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[Induction of immunity to amebiasis in subhuman primates by lysosomal antigen of Entamoeba histolytica. VI. Intrahepatic inoculation of virulent E. histolytica in monkeys].
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[Induction of immunity to amebiasis in subhuman primates by lysosomal antigen of Entamoeba histolytica. VII. Comments and conclusions].
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[Social medicine and community health. I. Introduction].
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[Immunization against Entamoeba histolytica. Current state and conclusive perspectives].
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[Induction of protective anti-ameba immunity with new antigens in the suckling hamster. a. Introduction].
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Activation of the alternative pathway of complement by Entamoeba histolytica.
A cytopathogenic effect was observed when Entamoeba histolytica was exposed to human sera from individuals with no clinical history or laboratory evidence of amoebiasis. Absorption studies showed that the effect was not due to natural antibodies. Studies performed using ethylenediamine tetracetic acid (EDTA), cobra venom factor (CoF) and heat-inactivation at 56 degrees C, indicated that the cytopathogenic effect was complement dependent. Furthermore, by using ethylene glycol tetracetic acid (EGTA) and Mg++, zymosan, heat-inactivation at 50 degrees C to destroy the activity of factor B of the alternative pathway, as well as electrophoretic studies with anti-human factor B, it was possible to determine that E. histolytica activated the properdin pathway. Finally, complement determinations indicated that incubation of E. histolytica with normal human serum consumed complement. The diminution in CH50 correlated with a consumption of C3 but not of C1, C4 and C2. It was concluded from these results that trophozoites of E. histolytica activate the alternative pathway of the human complement system.
[The research programs in the Mexican Institute of Social Security].
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[Induction of protective anti-ameba immunity with new antigens in the suckling hamster. e. Comments and conclusions].
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[Biological contaminants that affect the digestive system].
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