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Biomedical subjects

B Seaton

Publications and source records attributed to B Seaton.

16 recordsLinked to original sources
PubMed1987

Isolation of Haemophilus influenzae genes that suppress Escherichia coli polA mutations.

Haemophilus influenzae was found to produce a DNA polymerase that was similar to polymerase I of Escherichia coli. E. coli polA mutants were used as backgrounds for the selection of H. influenzae polA suppressor genes. Six different H. influenzae fragments were isolated that could suppress E. coli polA mutations. None of the suppressors appeared to encode the H. influenzae equivalent of the E. coli polA gene. One type of clone, represented by pGW41, caused a polymerase I activity to appear in a suppressed polA1 mutant. Plasmids from the pGW41 class contained two genes (pol-2 and pol-3) that were both required for polA suppression. Mutated nonsuppressing derivatives of the pGW41 class were used to create H. influenzae mutants that were deficient in polymerase I.

DNA Polymerase I↗
PubMed1978

Patterns of oestrogen and testosterone excretion during pregnancy in a gorilla (Gorilla gorilla).

Oestrogen and testosterone concentrations in the urine were determined by radioimmunoassay. Total oestrogen concentrations rose from 25 ng/mg creatinine in the non-pregnant animal to a mean of about 2 microgram/mg creatinine in the 50 days before term. There was a significant (P less than 0.001) correlation between oestrogen and testosterone concentrations until 11 days before parturition.

Animals↗
PubMed1978

Conflicts, trade-offs, and preference measurement.

The usefulness of preference measurement is extended by two techniques: the use of collapsed orthogonal arrays of factor combinations to reduce respondent time needed to indicate preferences among many factors and the use of monotonic analysis of variance to establish utility scales for individuals as well as for the group. Only main effects can be measured, but comparison of individual utility scales can aid the decision maker in gaining acceptance of decisions from group members whose preferences are not widely shared by the group. Use of these techniques is illustrated in a situation in which a few vocal members of a hospital medical staff were pressing the administrator for major changes in diagnostic facilities for out-patients.

Analysis of Variance↗
PubMed1975

Continuous determination of the oxygen dissociation curve for whole blood.

We report here the development of a new method that allows continuous determination of the oxygen dissociation curve for microsamples (600 mul) of whole blood under conditions of pH, pCO2, methemoglobin concentration, and 2,3-diphosphoglycerate content closely approaching those found in the circulatory system. The method consists of gradually oxygenating a blood sample by adding H2O2 in the presence of catalase (EC 1.11.1.6), to produce the reaction H2O2 leads to H2O + 1/2 O2. Because the total oxygen content of blood can be derived from the known rate of H202 addition and the pO2 is determined in the liquid phase by an oxygen electrode, the two functions (total O2 content) and (% oxygen saturation) vs. pO2 are simple to calculate. pCO2 and pH are controlled by adding base simultaneously with the gradual oxygenation of blood. The method described thus avoids the direct measurement of oxygen saturation of whole blood.

Autoanalysis↗
PubMedDate not supplied

Noncompliance among oral contraceptive acceptors in rural Bangladesh.

The levels of noncompliance among 175 oral contraceptive acceptors in a rural area of Bangladesh were monitored for three to four months. Unexpectedly high levels of noncompliance were observed, including both overconsumption and underconsumption of oral contraceptive pills. Although the study was designed to investigate only the levels of noncompliance and not the causes and consequences of noncompliance, some useful indications of the factors associated with noncompliance were obtained. The results indicate that noncompliance is a significant factor in family planning programs and, as such, warrants further in-depth investigation.

Bangladesh↗