Search PubMed⌕ Search

Biomedical subjects

B Schnitzer

Publications and source records attributed to B Schnitzer.

At least 73 records · Page 4Linked to original sources
PubMed1986

Site-dependent phenotypic heterogeneity in peripheral T-cell lymphoma.

Peripheral T-cell lymphomas constitute a heterogeneous population of postthymic T-cell malignancies. Characteristically, they present a varied phenotypic expression, which can be helpful in establishing the diagnosis. A case of a peripheral T-cell lymphoma in a 76-year-old man is described. The malignant cells in the skin and bone marrow were of the T4 (helper/inducer) phenotype, yet they did not express pan-T-cell antigens, such as T11, or functional E rosettes. In a biopsy specimen from a lymph node, however, the malignant cells had a helper/inducer phenotype and also expressed the pan-T-cell antigens T11 and Leu-5. Additionally, the malignant cells from the lymph node formed E rosettes. This study demonstrates the phenotypic heterogeneity of malignant T cells, which appears to be site-dependent.

Aged↗
PubMed1986

Storage and transportation of lymphoid tissue for immunophenotyping.

Immunoperoxidase staining of frozen sections is a cost-effective technic for immunophenotyping cells of lymphoid tissue. Because this procedure is not performed in many institutions, a simple method to transport fresh tissue to centers performing these studies is required. Tissues in saline at refrigerator temperature may be successfully transported. In addition, in order to minimize laboratory expenses, lymphoid tissue can be kept refrigerated in saline until permanent sections are examined and immunodiagnostic procedures become necessary. In this study reproducible immunophenotyping of 12 samples of lymphoid tissue stored up to seven days was achieved.

Antibodies, Monoclonal↗
PubMed1986

Comparison of morphologic features and mitotic rate to cytometrically determined DNA content of poorly differentiated lymphocytic lymphomas.

A direct correlation between the percentage of cells in S phase of the cell cycle and the clinical behavior of lymphocytic lymphomas of low, intermediate, and high grade malignancy has been described. The histopathologist has used the mitotic rate and other morphologic criteria such as size of cells and nuclear characteristics as predictors/indicators of the aggressiveness of a tumor. We compared the S phase values of 22 cases of poorly differentiated lymphocytic lymphoma (PDL) of the B cell type, using flow cytometric measurement of DNA content, to morphologic features and mitotic rate (MR). The 22 cases were divided into 3 histologic groups: nodular PDL composed of small, cleaved lymphocytes (11 cases); follicular mantle zone lymphoma and those of intermediate differentiation (6 cases); and "blastic" PDL (5 cases). In Group 1 there was excellent correlation of MR, percentage of cells in S phase, and proportion of large cells (transformed lymphocytes) per high power field (HPF). In Group 2, this correlation was not found between MR and percentage of cells in S phase in five of the six cases. The high S phase in this group did correlate with the large proportion of large cells found primarily in pseudofollicular proliferation centers and in remnants of true follicular centers. These cells may have a prolonged S phase and thus fewer mitoses were seen. In Group 3, although both MR and S phases were high, a direct correlation between them as noted in the Group 1 cases was not seen, but an excellent correlation of the high S phase and the number of blasts was present. The fact that three of the five patients in this group died rapidly (within less than 2 years of presentation) and the two survivors were experiencing rapid progression of disease, supports the concept that this group represents a clearly different, more aggressive subclass of PDL.

DNA↗
PubMed1986

Utility of Leu M1 monoclonal antibody in the differential diagnosis of Hodgkin's disease.

We conducted a retrospective study of the utility of Leu M1 monoclonal antibody staining of paraffin-embedded tissue in the differential diagnosis of Hodgkin's disease (HD). Forty-two cases of HD of various histologic types and 33 cases of non-HD lymphomas and hyperplasias were stained with Leu M1 using the avidin-biotin-peroxidase complex technique. Varying numbers, but not all Reed-Sternberg (RS) and Hodgkin's cells in all 42 cases of HD, were Leu M1 positive. These cases included seven examples of interfollicular HD and nine cases of lymphocyte-predominance HD, eight of which were nodular. Four of five cases of immunologically proved T-cell lymphoma contained Leu M1-positive RS-like cells, and Leu M1-positive RS-like cells were noted in two of five cases of non-HD lymphoma that were not phenotyped but were morphologically consistent with T-cell lymphoma. We concluded that Leu M1 staining is an aid in the diagnosis of HD, but it cannot be used to differentiate HD from T-cell lymphoma containing RS-like cells.

Antibodies, Monoclonal↗
PubMed1985

Flow cytometric analysis of DNA in diagnostic cytology.

Flow cytometric analysis of nuclear DNA content and cell surface immunologic phenotyping were used in the evaluation of cytologic samples obtained from four patients. In each sample, a lymphoid cell population was present, which was difficult to evaluate by traditional cytopathologic methods. In two of the cases, the flow cytometric demonstration of monoclonal populations of lymphoid cells characterized by abnormal amounts of nuclear DNA gave support to the cytologic interpretation of malignancy. In a third sample, a lymphoid cell population that could not be cytologically distinguished from malignant lymphoma cells of small lymphoid cell type was shown to be composed of euploid, polyclonal cells. In the fourth case, the demonstration of euploidy in morphologically distinctive cell populations was helpful in interpreting the fine-needle aspirate of the thyroid gland. The authors conclude that flow cytometry can be used to great advantage in the evaluation of cytologic samples as well as in predicting biologic behavior.

Adult↗
PubMed1985

Esophageal involvement in lymphoma.

Esophageal involvement by lymphoma in three patients, two with non-Hodgkin's lymphoma and one with Hodgkin's lymphoma are reported. In all three patients, there was discrete involvement of the esophagus not directly contiguous with the stomach. Esophageal involvement by lymphoma either as a primary disease or as manifestation of a disseminated disease is distinctly uncommon.

Adult↗
PubMed1984

The preparation of electronically sorted hematopoietic cells for visual analysis.

The authors describe a simple method of collecting and preparing electronically sorted hemic cells for visual analysis. Wright-stained preparations were obtained easily by this method and offered excellent preservation of morphologic features. Flow cytometer-derived data thus can be correlated with the morphologic characteristics of immunologically distinctive subsets of hemic cells.

Cell Separation↗
PubMed1982

Prolonged lifespans in female NZB/NZW mice treated with the experimental immunoregulatory drug frentizole.

Autoimmune female New Zealand Black/New Zealand White mice were treated with frentizole, an experimental immunosuppressive drug. Three groups of "young," 8-week-old mice received high-dose frentizole (80-84 mg/kg/day), low-dose frentizole (8 mg/kg/day), or no drug (controls); these mice were followed until spontaneous death. Three groups of "old," 24-week-old mice with established lupus-like disease were treated with high or low doses of frentizole. Old control mice received no drug. After 12 weeks of therapy, surviving old mice were killed. Beneficial therapeutic response was achieved when high-dose treatment was started at an early age; antiDNA values were suppressed, and longevity was prolonged significantly. Frentizole did not arrest the progression of renal disease in old mice. Glomerulonephritis and vasculitis were the most common causes of death in young and old animals. Twenty-nine percent of young, high-dose-treated mice died with neoplasms. Large glomerular deposits of IgG, IgM, and C3 were present in renal tissue from treated and control mice. Peripheral lymphocyte counts and mitogenic responses of spleen cells were not changed by treatment. The efficacy of frentizole in a murine model of lupus supports its usefulness as an immunoregulatory drug.

Age Factors↗
PubMed1982

Increased numbers of lymphocytes with single class surface immunoglobulins in reactive hyperplasia of lymphoid tissue.

Forty-seven lymph nodes and one stomach exhibiting reactive hyperplasia from 40 patients were examined by morphologic and immunologic methods. Twelve lymph nodes and one stomach from ten patients had a significantly increased number (greater than 25%) of lymphocytes, with single class surface immunoglobulins or plasma cells, and plasmacytoid lymphocytes secreting single class immunoglobulins. In three cases, a diagnosis of malignancy was subsequently made on the basis of morphological and immunological evidence of tumor in other tissue. Five patients had collagen disorders, two had a persistent unexplained hypergammaglobulinemia, and two had no recognized associated disease. The significance of an increase of B lymphocytes with single class surface immunoglobulins in these cases is presently not clear, but patients with similar findings should have extensive investigation and persistent follow-up.

Adult↗
PubMed1982

Splenomegaly without an apparent cause.

Twenty-eight patients undergoing splenectomy for splenomegaly of unclear causation are presented. The cause of splenomegaly was determined at splenectomy in seven patients, none of whom had lymphoma. In three additional patients, disease associated with splenomegaly developed in the follow-up period after splenectomy. During the entire follow-up period, only one instance of lymphoma has developed. Especially in young patients who have a history of recent infection, a delay in performing splenectomy for splenomegaly is indicated.

Adolescent↗
PubMed1980

Resistance to therapy in mature Palmerston North mice treated with cyclophosphamide or hydrocortisone sodium succinate.

Inbred Palmerston North (PN) mice are a newly recognized model of systemic lupus erythematosus. In this study PN mice with established autoimmune disease were treated until death with cyclophosphamide (8 mg/kg/day) or hydrocortisone (10 mg/kg/day). These doses had previously been found to prevent or suppress disease in another lupus model, the NZB/NZW mouse. In the PN strain, autoantibodies, severity of glomerulonephritis, and longevity were not influenced by treatment. Furthermore, the incidence of neoplasms was not increased in PN mice receiving prolonged therapy with immunosuppressive drugs. Unlike NZB/NZW mice, PN mice were resistant to the effects of Cyclophosphamide and hydrocortisone.

Animals↗