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Biomedical subjects

B Scharf

Publications and source records attributed to B Scharf.

14 recordsLinked to original sources
PubMed1992

Chromophore of sensory rhodopsin II from Halobacterium halobium.

The photoreceptor sensory rhodopsin II (sR-II) was enriched 120-fold from cell membranes of Halobacterium halobium. The final preparation yields sR-II with a specific content of 3 nmol of sR-II/mg of protein. The spectroscopic measurements were performed on the enriched photoreceptor solubilized in digitonin. In the absolute absorption spectrum of the partially purified receptor, the main peak in the visible range corresponded to sR-II with a maximum at 488 nm. Cytochromes contributed to the spectrum only in a minor band at 415 nm. The extinction coefficient of sR-II was estimated from difference spectra during bleaching with hydroxylamine to be 48,000 M-1 cm-1. The reduced chromophore displayed a pronounced fine structure which is due to the coplanarity of the retinyl residue. The isomeric composition of the chromophore from the enriched photoreceptor was determined in retinal extracts in HPLC. The dark-adapted sR-II contains 80% all-trans- and 20% 13-cis-retinal. After illumination, the ratio changed to 1:1, indicating a trans-cis isomerization during the photocycle of sR-II.

Archaeal Proteins↗
PubMed1992

Biochemical and photochemical properties of the photophobic receptors from Halobacterium halobium and Natronobacterium pharaonis.

The phototaxis of Halobacterium halobium is initiated by two photoreceptors, the sensory rhodopsins sR-I and sR-II. An sR-II-like pigment has also been described in Natronobacterium pharaonis. In this work it was shown that N. pharaonis cells are repelled by light with a wavelength of 500 nm. A further comparison of membrane preparations from H. halobium (mutant D1) containing only sR-II and from N. pharaonis [strain SP1(28)] with a chromophoric protein (psR-II) resembling sR-II revealed substantial similarities. The biochemical and photochemical properties of the pigments are quite similar, with psR-II being more stable to external conditions such as pH and ionic strength of the buffer. Both pigments are bleached by low concentrations of hydroxylamine and can be reconstituted by the addition of all-trans-retinal. The absorption spectrum of psR-II is quite similar to sR-II including the shoulder on the short-wavelength side. After light excitation sR-II and psR-II undergo photocycles with at least three intermediates. The earliest intermediate has an absorption maximum above 520 nm and decays to a species which has a characteristic absorption (approximately 380 nm) of a deprotonated Schiff base. The final step is the regeneration of the original ground state via a red-shifted intermediate absorbing around 540 nm. From this cumulative evidence it can be concluded that, not only sR-II, but also the pigment from N. pharaonis is a photophobic photoreceptor.

Archaea↗
PubMed1991

Effective attenuation of signals in noise under focused attention.

When attending to a tone at a given frequency, listeners are most sensitive to that tone and others within a restricted band of frequencies surrounding it. This region of enhanced sensitivity defines the attention band that was measured in two experiments using a modified version of the probe-signal method of Greenberg and Larkin [J. Acoust. Soc. Am. 44, 1513-1523 (1968)]. Experiment 1 showed that at five center frequencies, from 0.25 to 4.0 kHz, the shape of the attention band resembles that of the auditory filter as inferred from notched-noise masking experiments by other investigators. The width of the attention band is close to the critical band at higher frequencies, but only half as wide at 0.25 and 0.5 kHz. Experiment 2 produced psychometric functions for unattended probe tones at least 0.23 kHz away from a fully attended, 1-kHz target tone. From these functions, the effective attenuation, measured as the threshold difference between the 1-kHz target and the probes, was estimated to be 7 dB; the amount of attenuation appeared to be about the same regardless of how far the probe frequency was from the attended band. One interpretation of these results is that bands centered on the unattended tones contribute to the decision process with some small but measurable weight and are not entirely ignored.

Acoustics↗
PubMed1990

The loudness of sounds that increase and decrease continuously in level.

A sound at a low level is heard as much softer after having decreased continuously from higher levels than if presented after a period of silence at that same low level. Canévet [Acustica 61, 256-264 (1986)] demonstrated this phenomenon for a tone that (1) decreased from 65 to 20 dB in 180 s; he also presented a tone that (2) increased from 20 dB, or (3) was presented as pairs of bursts at various levels in random order. Below about 40 dB, loudness changed most rapidly in the decreasing condition so that, at 20 dB, the tone was judged ten times softer than in conditions (2) and (3). In the present experiments, magnitude estimation was used to examine the possible role of judgmental biases and adaptation in this rapid loudness decline, which we call decruitment. Results show that decruitment did not come about because subjects made many successive loudness judgments; loudness declined as much when a tone was judged only twice, at the beginning and end of its 180-s decrease. In contrast, interrupting the decreasing tone so that it was heard only at 70 dB and 160 s later at 30 dB greatly diminished the decruitment. Similarly, pairs of 500-ms tone bursts presented at successively lower levels instead of continously decreasing did not show decruitment, suggesting that sequential biases are irrelevant. The likely cause of decruitment is sensory adaptation.

Attention↗
PubMed1987

On the relation between the growth of loudness and the discrimination of intensity for pure tones.

The intensity jnd is often assumed to depend on the slope of the loudness function. One way to test this assumption is to measure the jnd for a sound that falls on distinctly different loudness functions. Two such functions were generated by presenting a 1000-Hz tone in narrow-band noise (925-1080 Hz) set at 70 dB SPL and in wideband noise (75-9600 Hz) set at 80 dB SPL. Over a range from near threshold to about 75 dB SPL, the loudness function for the tone is much steeper in the narrow-band noise than in the wideband noise. At 72 dB SPL, where the two loudness curves cross, the tone's jnd was measured in each noise by a block up-down two-interval forced-choice procedure. Despite the differences in slope (and in sensation level), the jnd (delta I/I) is nearly the same in the two noises, 0.22 in narrow-band noise and 0.20 in wideband noise. The mean value of 0.21 is close to the value of 0.25 interpolated from Jesteadt et al. [J. Acoust. Soc. Am. 61, 169-176 (1977)] for a 1000-Hz tone that had the same loudness in quiet as did our 72-dB tone in noise, but lay on a loudness function with a much lower slope. These and other data demonstrate that intensity discrimination for pure tones is unrelated to the slope of the loudness function.

Acoustic Stimulation↗
PubMed1986

Loudness reduction and adaptation induced by a contralateral tone.

An intermittent tone in one ear may induce a large decline in the loudness of a continuous tone in the contralateral ear [Botte et al., J. Acoust. Soc. Am. 72, 727-739 (1982)]. To uncover the basis for this induced loudness adaptation, the method of successive magnitude estimations was used to measure the loudness of a test tone in one ear during and after a single presentation of a brief inducer tone in the contralateral ear. Duration and frequency of the inducer were varied. The frequency of the test tone was set at 500, 1000, or 3000 Hz. Both inducer and test tones were at 60 dB SPL. When the inducer lasted 5 s or more and was at the same frequency as the test tone, the loudness of the test tone was reduced by 80% to 100% while the inducer was on. As the inducer frequency moved away from the test tone, the loudness reduction declined gradually except for a more marked drop at the point where the frequency separation exceeded the critical bandwidth. Loudness remained depressed after the inducer went off. Additional measurements showed that the amount of loudness reduction corresponded closely to the measured movement of the inducer's sound image away from the center of the listener's head (decentralization).

Acoustic Stimulation↗
PubMed1986

[Auditory localization and speech perception in noise. Preliminary study concerning 5 cases of perceptual deafness].

The experiments reported here examine the psychoacoustical bases for poor speech perception in noise by persons with sensorineural impairment. Two major hypotheses are tested. First, because persons with cochlear impairment are less able than normal-hearing persons to separate out incoming signals on the basis of spectral differences (a deficit referred to as reduced frequency selectivity), they are less able to localize one sound in the presence of other sounds. Second, this reduced localization ability makes it difficult for the hearing-impaired person to take advantage of the spatial separation of a target speech source and other interfering sources. Such separation is common in real environments and facilitates speech perception by normal-hearing persons. Tests of these hypotheses are conducted by means of detailed psychoacoustical measures of frequency selectivity, of localization and speech perception under masking.

Adolescent↗
PubMed1985

Simple and induced loudness adaptation.

Simple loudness adaptation is the decrease in loudness that takes place when a continuous sound is presented alone for a period of time. Simple adaptation normally occurs only when a sound is soft to begin with, no more than 30 dB above threshold; except for some persons with a retrocochlear lesion, sounds above 30 dB SL do not diminish in loudness over time. However, adaptation can be induced in at least two ways: (1) A steady sound to one ear, presented together with an intermittent sound to the contralateral ear, decreases in loudness by 50-60% within 3 min. (2) An otherwise steady sound that is intermittently increased in level by at least 5 dB becomes softer during its weaker periods. When, for example, a 40-dB tone is increased every 20 s to 60 dB for 15 s, its loudness decreases by about 50% within 3 min. We report measurements of both simple and induced adaptation on 10 persons listening to a 1 000-Hz tone via earphones or from a loudspeaker. The results provide an overview of both types of adaptation. They also permitted a correlational analysis that reveals some of the similarities and differences between the two kinds of adaptation.

Adaptation, Physiological↗
PubMed1978

Comparison of normal and impaired hearing. I. Loudness, localization.

Impaired hearing is characterized by high thresholds and reduced loudness. Loudness, however, may quickly recover as it increases rapidly from an elevated threshold. This rapid growth, known as loudness recruitment, is a sign of cochlear impairment and is generally not seen in conductive or retrocochlear impairment. Loudness recruitment means that the hard-of-hearing person detects small changes in intensity near his elevated threshold but he probably does no better than a normal listener at the same SPLs. Recruitment is often accompanied by reduced loudness summation, which means that the loudness of a band of noise does not increase as much with increasing bandwidth as in normal hearing. This reduced summation of loudness is probably why the cochlearly impaired ear has nearly the same threshold for the acoustic reflex to pure tones as to wide-band noise, whereas the normal ear has a much lower threshold to wide-band noise. Corresponding differences between normal and impaired hearing are not found in auditory localization. Rather, the evidence suggests that persons with residual hearing learn to localize sounds reasonably well. Even the inability of many hearing impaired persons to understand a speaker in a noisy environment may result more from a failure of frequency analysis rather than of localization.

Auditory Threshold↗
PubMed1978

Comparison of normal and impaired hearing. II. Frequency analysis, speech perception.

Frequency analysis covers two separate listening tasks, one involving frequency discrimination, the other frequency selectivity. Discrimination refers to the ability to distinguish one frequency from another. Selectivity refers to the ability to hear one frequency in the presence of other frequencies. Selectivity is critical to the understanding of speech which comprises sounds containing many different frequencies. To understand speech easily, the listener must be able to analyze speech sounds into their component frequencies, especially formants. The hard-of-hearing person is probably less able to make that analysis, but we know surprisingly little about either discrimination or selectivity in hearing impairment. Existing evidence does suggest that both discrimination and selectivity are reduced in cochlear impairment so that such patients need a bigger frequency difference to discriminate between two tones and they have a wider critical band. A widened critical band would be expected to make it very difficult for the severely impaired person to understand speech under all listening conditions; it would make it difficult for the moderately impaired person to understand speech in a noisy background, unless the signal-to-noise ratio is improved as is possible by appropriate amplitude compression in hearing aids.

Audiometry, Pure-Tone↗
PubMed1976

Critical band in auditory lateralization.

A new and powerful procedure for determining frequency analysis in the auditory system, as evidence by the critical band, is described. The onset time difference, delta T, needed to lateralize 30-msec tone bursts toward the leading ear was measured as a function of the frequency difference, delta F, between the brust in one ear and the burst in the other ear. When delta F was less than the critical band, threshold delta T was constant at 100 mu sec or less, depending on center frequency; beyond the critical band, delta T increased with delta F. These dichotically measured critical bandwidths increased from 110 Hz at a center frequency of 500 Hz to 1100 Hz at a center frequency of 6000 Hz. They were unaffected by varying signal level from 25 to 80 dB or signal duration from 10 to 300 msec. The sam e critical-band values have been measured with monaural stimuli in loudness summation, maskin, detection, phase perception, consonance, and so forth.

Acoustic Stimulation↗