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Biomedical subjects

B Sarcević

Publications and source records attributed to B Sarcević.

At least 19 recordsLinked to original sources
PubMed1996

Detection of human papillomavirus (HPV) type 6, 16 and 18 in head and neck squamous cell carcinomas by in situ hybridization.

Seventy seven squamous cell carcinomas (10 oral cavity, 15 tongue, 26 pharynx and 26 larynx), with different grading, were analyzed for the presence of HPV DNA by in situ hybridization. Positive signals were found on the nuclei of cancer cells in 25 (32.5%), in the epithelia adjacent to squamous cell carcinomas in 2 (8.7%), and in the resected margins in 1 (4.3%) case. HPV DNA positive signals were obtained in 42% of laryngeal, 34% of pharyngeal, in 20% of oral, and 20% of tongue carcinomas. Out of 25 HPV positive carcinomas a single HPV type was detected in at least 11 (44%), and double or multiple infection in 9 (36%) cases; altogether, HPV 6 DNA was determined in 15 (60%), and HPV 16 and/or 18 DNA in 17 (68%) head and neck tumors. The detection rate of HPV 6 was lower than of HPV 16 and/or 18 for tumors in oral cavity, tongue and larynx. Out of 25 HPV DNA positive carcinomas 21% were graded as G1, 27% as G2, and 44% were G3. The results indicate that HPV may be involved in the pathogenesis of head and neck squamous cell carcinomas.

Adult↗
PubMed1996

Fibromatosis of male breast.

Fibromatosis is a relatively benign, though locally invasive neoplasm. About 100 cases of fibromatosis of the breast have been reported to date, none of them in a male. We present a 60-year-old man who was admitted to the University Hospital for Tumors in Zagreb, Croatia, on May 25, 1992, with a 3-month history of gradually enlarging, painless subcutaneous mass, 6 x 5 cm, that was located in the upper outer quadrant and partly beneath the areola of his left breast. The diagnosis was confirmed by immunoperoxidase studies, that showed positivity for vimentin, whereas the staining for cytokeratin AE1, glial fibrillar acid protein (GFAP) and collagen IV was negative. The tumor was removed by en bloc resection, and the greater pectoral muscle was spared. Today, 3 years after the operation, the patient is alive and in good health.

Breast Neoplasms, Male↗
PubMed1996

Locally recurring primary myxoma of the breast: an evidence of malignant alteration.

Breast is an extremely rare localization of myxoma. To date, only five cases have been reported, including one case of nerve sheath myxoma. In this report, the authors present a 37-year-old female patient who was admitted to the University Hospital for Tumors in Zagreb, Croatia, in 1979, with a 2-month history of gradually enlarging, painless subcutaneous mass in her right breast. On physical examination, a firm, relatively well circumscribed mass measuring 3 x 2 cm was detected in the upper outer quadrant and partly beneath the areola. The mass was freely movable to the overlying skin, causing no retraction. There was no family history of a breast disease or cancer. Patient's past history revealed no major illnesses or trauma. The tumor was removed by simple local excision, but it recurred locally in three episodes between 1981 and 1986. After that, the tumor underwent malignant alteration to myxosarcoma on its fourth (in 1992), and to myxoid liposarcoma on its fifth recurrence (in 1993). No such sequence of events has been reported in the literature so far.

Adult↗
PubMed1995

Medullary carcinoma of the thyroid: histomorphological, histochemical and immunohistochemical analysis of twenty cases.

Histomorphological patterns of twenty primary medullary carcinomas of the thyroid were studied by light and polarized microscopy in relation to the content of calcitonin and thyroglobulin determined by the immunoperoxidase method: Out of 20 tumors, 10 showed classical, 7 glandular and 3 insular histomorphological pattern. In 19/20 cases, the cytoplasm of tumor cells contained various amounts of calcitonin, and the intensity of Immunoreaction was strong in 2/19, moderate in 7/19 and weak in 10/19 cases. Tumor stroma contained calcitonin in 7/20 cases. In 1/20 case, which did not show calcitonin immunoreactivity in the cell cytoplasm, the stroma contained a considerable amount of calcitonin. Thyroglobulin immunoreactivity was found in 4/20 tumors, 2 of them with classic and 2 with glandular histomorphological picture only in the cytoplasm of tumor cells. These tumors are considered medullary carcinomas with thyroglobulin immunoreactivity, since they do not fulfil the WHO criteria for "mixed medullary-follicular carcinomas".

Adult↗
PubMed1993

Immunocytochemical reactivity of a mouse monoclonal antibody CDI 315B raised against human breast carcinoma.

Our previous report revealed the production of monoclonal antibody (MoAb) CDI 315B by immunization of mice with tumor extract proteins of human invasive ductal breast carcinoma. In the present study we report on the immunocytochemical reactivity of this MoAb with formalin or methacarn fixed, paraffin embedded tissue sections and also with cell cultures. Among breast tissues, positive staining was detected in 88% (64 of 73) of primary breast carcinomas, 77% (7 of 9) of metastatic lymph nodes, 24% (8 of 33) of benign breast disease and 15% (2 of 13) of normal breast tissue. No immunostaining was detected with several other tumors, with the exception of melanoma, where 63% (5 of 8) of positive staining was found. On in vitro cell lines, positive reaction was detected only with breast carcinoma and melanoma cells, but not with other examined cell lines. On benign breast disease tissue sections, positive reaction was detected in areas with cell hyperplasia. On normal breast tissue sections MoAb 315B stained the epithelial cells of terminal ductuli. Since the MoAb 315B recognized some antigen present in the cytoplasm of most breast carcinoma cells, this MoAb may have potential application in diagnosis and management of breast cancer.

Adenofibroma↗
PubMed1989

[The significance of certain histologic parameters in the differentiation and diagnosis of Hürthle cell adenoma and carcinoma of the thyroid gland].

Hürthle cell tumors of the thyroid gland are rare. Their natural history remains incompletely understood what results in their different classification and therapy. The differentiation of benign from malignant Hürthle cell tumors is possible with classical, traditional histologic methods. Authors have analysed 53 patients with Hürthle cell tumor of the thyroid gland which were diagnostically evaluated and treated at the Central Institute for Tumors and Allied Diseases in Zagreb from 1975 to 1988.

Adenoma↗
PubMed1988

Estrogen and progesterone receptors as prognostic factors in breast cancer.

The relation between estrogen receptors (ER) and/or progesterone receptors (PgR) and some clinical factors such as tumor size, axillary node involvement, histological tumor grade, and disease-free interval (DFI) in 500 patients with operable (TNM stage I-III) breast cancer was studied. ER-positive (ER+) tumors were commoner in older patients, whereas PgR-positive (PgR+) tumors were similarly distributed within the age groups. The concentration of ER+ protein also increased with age in contrast to PgR+ protein concentration. However, receptor status was not associated with menopausal status independently of age. Axillary node involvement influenced neither ER nor PgR status, but there was a statistically significant relation between tumor size and positivity of ER or PgR. There was no association between histologic tumor grade and either steroid receptor phenotype. DFI was longer in patients with ER+ than those with ER- tumors, independently of axillary nodal status. The positivity of PgR in patients with ER+ tumors contributed to an even longer DFI, suggesting that the combination of ER/PgR is a better indicator of DFI than ER or PgR alone.

Adult↗
PubMedDate not supplied

[Oncogenes and growth factors in human epithelial tumors: their importance in diagnosis and prognosis].

The accumulation of the genetic alterations, the activation of oncogenes and the inactivation of tumor suppressor genes, play a critical role in the induction of the malignant tumours. In human solid tumors, oncogenes and growth factors are found at any of the different stages in carcinogenesis. The clinical results of these observations are the demonstration of some relations between activation and expression of the oncogenes and growth factors and clinical features or prognosis. Furthermore, they seem to permit definition of premalignant conditions or populations of high risk of disease.

Breast Neoplasms↗
PubMedDate not supplied

[Adenomas of the thyroid gland--criteria for pathohistologic diagnosis].

Adenomas are the frequent tumors of the thyroid gland, which have different histological types, distinct criteria for pathohistological diagnosis, and distinct criteria for differentiation the follicular adenoma from follicular carcinoma of the thyroid gland. Authors have analyzed 63 patients with follicular adenomas of the thyroid gland which were diagnosed and treated at the Central Institute for Tumors and Allied Diseases in Zagreb in the period from 1986 to 1987, and on which had been done serial cuttings of the tumors for pathohistological diagnosis.

Adenoma↗
PubMedDate not supplied

[Strumal carcinoid tumor of the ovary--histologic and electron microscopy characteristics of the tumor].

Strumal carcinoid tumor is a very rare primary tumor of the ovary in which carcinoid is admixed with tissue resembling thyroid follicles. We report a 44-year-old women with benign strumal carcinoid tumor of the ovary and without any clinical symptoms. Histochemical and electron microscopical examinations of the tumor showed intracytoplasmic dense core secretory granules, indicating that this neoplasm is a pure carcinoid tumor with acinar differentiation resembling thyroid tissue.

Adult↗
PubMedDate not supplied

[Angiosarcoma of the breast--histopathologic and electron microscopy picture].

The authors describe angiosarcoma of the right breast, together with the pathohistologic and electron microscopic analysis and survey of the literature of this rare tumor. The greatest tumor diameter was 4.5 cms. Mastectomy and the axilla's dissection was performed and additionally, radiotherapy was undertaken. In the first biopsy the wrong diagnosis of ductal invasive carcinoma was made. Three years later a metastasis appeared in the left axilla when angiosarcoma was established. The revision of primary tumor of the right breast showed that previously it was angiosarcoma of the right breast, too. In this article the authors direct attention to the possibility of wrong diagnosis of benign or malignant tumor and list the results taken from the world literature. Following those results, the wrong diagnoses were made in 37% of the cases with breast angiosarcoma.

Breast Neoplasms↗
PubMedDate not supplied

[The importance of clinical and histologic variables in the prognosis of medullary carcinoma of the thyroid gland].

Patients with medullary thyroid carcinoma (MTC) have aroused considerable interest ever since Horn (1951), Hazarde et al. (1959) and Williams (1966) described MTC as a separate clinicopathologic entity, because this tumor occurs both in families and in association with neural and endocrine abnormalities. This interest also underlines our clinicohistologic study of 31 patients with a histologically demonstrated MTC. The patients have been analyzed at the Central Institute for Tumors and Allied Diseases in Zagreb from 1969 to 1988. The youngest patient was 17, and the oldest one 85 (average 45 years). None of the patients had thyroid carcinoma in his/her family history, or any association with pheochromocytoma or multiple skin and mucosal neurinoma. The men to women ratio was 1:0.94. The prognostic significance of some clinical and histologic variables in patients affected by medullary thyroid carcinoma have been studied. Treatment involved total thyroidectomy with or without neck dissection. Some of the patients were subsequently irradiated or administered chemotherapeutic agents. The data obtained in the study were statistically processed and the statistical differences between the survival rates of the various patient groups were determined by the logrank-test. Of the studied variables (sex, age, tumor size, cervical metastases, tumor structure, cell type, stroma, calcification, number of mitoses, necrosis, bleeding, amyloid content), the following were found to have a prognostic significance: age, tumor size and regional lymph node metastases.

Adolescent↗
PubMedDate not supplied

[Solitary extramedullary plasmacytoma of the larynx].

Solitary extramedullary plasmacytoma (EMP) is histologically rare larynx tumor. Of the 620 treated larynx carcinomas, extramedullary plasmacytoma makes up 0.1% of larynx carcinomas recorded in our case reports. The symptoms of larynx EMP are like other tumor symptoms of the same site. Clinically, solitary extramedullary plasmacytoma is like a cyst. It is epithelialized and radiates grey light. Findings obtained by biopsy and extemporary histology did not confirm plasmacytoma. The diagnosis was made by histochemical procedure.

Aged↗
PubMedDate not supplied

[Relation between grades of histologic differentiation and hormone receptors in breast cancer].

The relationship between the grade of histologic differentiation of the tumor and estrogen (ER) and progesterone (PgR) receptor values was analyzed in 261 patients with breast cancer of the invasive duct type. There was a statistically significant difference in concentration and incidence of positive and negative ER and PgR with regard to histologic grade. The concentration and number of positive hormone receptors increased with better differentiation of the tumor. A statistically significant correlation between histologic grade, hormone receptor values and axillary nodal involvement was obtained only in patients with no metastases to axillary lymph nodes.

Breast Neoplasms↗