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Biomedical subjects

B Samuelsson

Publications and source records attributed to B Samuelsson.

At least 343 records · Page 19Linked to original sources
PubMed1970

Gas--liquid chromatography--mass spectrometry of synthetic ceramides containing 2-hydroxy acids.

Ceramides containing either sphingosine or sphinganine and one of the 2-hydroxy acids, 14h:0, 16h:0, 18h:0, 20h:0, 22h:0, 24h:0, and 26h:0 were prepared and separated by gas chromatography as the 1,3,2'-tri-O-tri-methylsilyl derivatives. Mass spectrometric analyses of these derivatives showed that the ions formed on electron impact can be used to determine unequivocally the structures of the long-chain base and the fatty acid residue in the ceramide. Proposed structures of ions and the mechanisms of reaction of their formation are supported by mass spectra of homologous derivatives, by deuterium labeling experiments, and by high-resolution on mass spectrometry.

Amino Alcohols↗
PubMed1969

Prostaglandins: enzymatic analysis.

By, means of al specific nicotinamide-adeninie dinucleotide-dependent prostaglanidin dehydrogenase from swine lung, an enizymatic method has been developed for the assay of prostaglandins. The method permits analysis with a lower limit of 10(-12) mole of prostaglandin.

Animals↗
PubMed1969

Gas--liquid chromatography-mass spectrometry of synthetic ceramides.

Two series of ceramides with either sphingosine (sphing-4-enine) or sphinganine as base and with one of the saturated fatty acids C(16), C(18), C(20), C(22), C(24), C(26), or oleic acid were analyzed as the 1,3-di-O-trimethylsilyl ether derivatives by gas chromatography-mass spectrometry. The fragments formed on electron impact can be divided into three main groups, namely "molecular weight fragments," "long-chain base fragments," and "fatty acid fragments." The m/e values of these fragments can be used to determine unequivocally the structures of the long-chain base and fatty acid of a ceramide derived from a sphingolipid.

Chromatography, Gas↗
PubMed1969

Separation and identification of ceramides derived from human plasma sphingomyelins.

Sphingomyelins from human blood plasma have been converted into ceramides by enzymatic hydrolysis with phospholipase C. After acetylation the ceramides were fractionated by thin-layer chromatography on silica gel containing silver nitrate. Four main fractions obtained by this method were subsequently converted to di-O-trimethylsilyl ether derivatives and separated by gas-liquid chromatography on 1% OV-1. 2-11 components could be distinguished in each of the four fractions. The major fractions emerging from the gas chromatograph were analyzed by mass spectrometry and their main molecular species were identified. Two of the gas chromatographic fractions contained essentially pure molecular species, namely N-tetracosenoyl sphingosine and N-tetracosenoylsphinga-4, 14-dienine.

Acetates↗