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Biomedical subjects

B Ruf

Publications and source records attributed to B Ruf.

At least 91 records · Page 5Linked to original sources
PubMed1987

Fatal Legionella pneumonia: retrospective examination of lung tissue using direct and indirect fluorescent-antibody methods.

Lung tissue sections of patients who died of pneumonia within one year were screened retrospectively for Legionnaires' disease bacteria using simultaneously a direct fluorescent antibody test (DFA) and an indirect fluorescent antibody system (IFA). The IFA has shown to provide a rapid screen for the increasing number of known Legionella species and serogroups recently. In 10/168 (6%) of our cases legionellae were the pneumonia causing agents detected with both methods. Although we should have been able to detect 25 serological variants of Legionella species and serogroups with the IFA, we found Legionella pneumophila serogroup 1 only. The IFA test has proven to be a reliable means for the screening of lung tissue sections.

Aged↗
PubMed1986

[Neurologic symptoms in Legionella infection. A case report of the clinical aspects and diagnosis of legionellosis].

In patients with infection from Legionella pneumophila, it must be taken into consideration that the nervous system may be involved. This involvement can precede the pneumonia by several days. Headache, acute mental status changes and cerebellar dysfunctions are the most common neurological symptoms. In the case reported, extrapyramidal disturbances were also observed that are rarely found in association with Legionella infection. There are grounds for the assumption that Legionella toxins cause impairment to the nervous system. The neurological symptoms are not specific enough to allow differentiation of this infection from pneumonia of other etiologies.

Athetosis↗
PubMed1986

Rapid detection of Legionella antigen in tissues and body fluids.

We report on a study in which a modification of the enzyme-linked immunosorbent assay (ELISA) using solid-phase microtiter plates has been applied to detect Legionella pneumophila (serogroup 1) antigen in urine, secretions and tissue homogenates. The radioimmunoassay (RIA) and ELISA techniques were compared with respect to sensitivity and range of detection of individual species' antigens. We present results of adsorption studies in which positive specimens were treated with specific IgG fractions raised in the rabbit against individual Legionella species. The advantage of the rapid ELISA or RIA diagnosis over the more time-consuming diagnostic measures is discussed.

Animals↗
PubMed1982

[Penetration of ceftizoxime (FK 749) into the cerebrospinal fluid (author's transl)].

After the cytology and the chemistry of the CSF had returned to normal, the concentration of ceftizoxime was determined in the CSF and in the serum of 27 patients who had suffered from purulent or serous meningitis. The patients were divided into four groups. The concentration in the CSF was mean = 0.87 mg/l two hours after a bolus injection of 2 g ceftizoxime. After an infusion of 2 g ceftizoxime over 30 minutes, the concentration in the CSF was mean = 0.32 mg/l one hour and mean = 0.99 mg/l two hours after the infusion was started. The highest concentrations in the CSF were obtained six hours after a bolus injection of 2 g ceftizoxime. The peak value was 4.3 mg/l and the mean 1.78 mg/l. Based on these pharmacokinetic data, additional injections could have a cumulative effect on the concentration of ceftizoxime in the CSF. With the exception of Staphylococcus sp., Pseudomonas sp. and some anaerobes, it can be expected that ceftizoxime will be effective against other organisms causing meningitis. The results of clinical applications remain to be seen.

Cefotaxime↗
PubMed1976

[Longtime therapy of congenital factor XIII deficiency using factor XIII concentrate].

Factor XIII was determined by enzymatic and immunochemical methods in 3 patients with congenital factor XIII deficiency. Factor XIII activity measured by trans-glutaminase assay was below 1% of normal value in each of these cases. Immunelectrophoresis determination revealed the absence of the functionally active subunit A, whereas subunit S was only slightly diminished (30 to 50% of the normal value). Substitution with factor XIII concentrate caused a parallel increase of factor XIII activity and subunit A concentration. No uptake of factor XIII activity or of subunit. A by platelets could be demonstrated. Despite discontinuous substitution over a period of six years no antibody against factor XIII activity could be demonstrated in one patient with congenital factor XIII deficiency.

Antibodies↗
PubMedDate not supplied

Effectiveness of the macrolide clarithromycin in the treatment of Mycobacterium avium complex infection in HIV-infected patients.

In a randomized double-blind study, nine mycobacteremic patients with AIDS-related disseminated Mycobacterium avium complex (MAC) infection received clarithromycin or placebo in addition to a basic regimen that included isoniazid, ethambutol and clofazimine. All four patients receiving clarithromycin showed blood culture conversion and clinical response. Of the five patients treated without clarithromycin, two showed resolution of mycobacteremia and clinical response, while another two died without having shown response. The remaining patient deteriorated until a switch from placebo to clarithromycin led to blood culture conversion and rapid clinical improvement. After finishing six weeks of intensive treatment, clarithromycin was given in an open maintenance phase to all patients, initially in combination with rifabutin for 24 weeks and then alone. One patient had a relapse of MAC infection while receiving clarithromycin alone. The relapse was associated with acquired resistance to the drug. Clarithromycin appears to be a promising component of multi-drug therapy for patients with MAC infection. Monotherapy can lead to drug resistance.

AIDS-Related Opportunistic Infections↗