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Biomedical subjects

B Roy

Publications and source records attributed to B Roy.

At least 109 records · Page 6Linked to original sources

A comparison of three skating techniques and the diagonal stride on heart rate responses and speed in cross-country skiing.

The purpose of this study was to compare the heart rate responses and the speed of three different skating techniques and the diagonal stride in cross-country skiing. The subjects were ten elite male cross-country ski racers, 16 to 25 years of age. They skied a 3.04-km loop of various terrain four separate times, using a different technique for each trial. A thirty-minute recovery period was allowed between each trial. Heart rate and skiing velocities were analyzed over a flat, an uphill, and a downhill section, as well as for the total loop. No significant differences (p greater than 0.05) were found in heart rate values between the four techniques on all sections of the course, while heart rates were significantly different over the three different sections. Skiing velocities were significantly different (p less than 0.05) between the diagonal stride and all skating techniques, the diagonal stride being the slowest technique, and this, for all sections. Energy costs while skiing were estimated on the basis of individual HR/VO2 curve determined under laboratory conditions. Since no differences in HR were found between the four techniques, no differences in energy costs were found. These energy costs represented between 69 and 73% of max VO2, and between 87 and 89% of max HR for the 3.04-km loop. Thus, for the same estimated energy expenditure, greater speeds (16%) were achieved with the skating techniques than with the diagonal stride.

Acceleration↗

Orientation and moment arms of some trunk muscles.

The orientations and moment arms of nine trunk muscles: latissimus dorsi, iliocostalis, longissimus dorsi, obliquii, rectus and transversalis abdominis, quadratus lumborum, and iliopsoas were obtained on embalmed specimens. Each muscle was considered as one or several, linear or curvilinear, lines of action. Origins, insertions, and points along the length of main bundles were digitized for each muscle using a three-dimensional digitizer. The results obtained from seven male specimens are presented and will permit more refined biomechanical models.

Abdominal Muscles↗

Stimulatory effects of retinoic acid on tumor growth and serum insulin-like growth factor-1 in rats bearing estrogen-responsive pituitary tumor MtT/Se.

MtT/Se is one of 4 cell lines derived from an estrogen-dependent pituitary tumor, MtT/F84. The main difference between these tumor types is that MtT/F84 secretes both growth hormone (GH) and prolactin (PRL) whereas MtT/Se secretes only GH. MtT/Se grew slowly in ovariectomized (ovex) rats, but tumor growth was much faster in estrogen-treated ovex rats. Effects of dietary retinoic acid (RA) on tumor growth, serum GH and insulin-like growth factor-1 (IGF-1) levels were examined in ovex rats. Latency of tumor growth was shortened, and tumor take and weight were promoted by all-trans RA both in the presence and absence of exogenous estrogen. Serum GH and IGF-1 levels became increased in tumor-bearing rats whereas PRL levels remained unchanged. Serum IGF-1 levels exhibited a good correlation with tumor weights (r = 0.84). Our results suggest a close relationship between increase of tumor weight and stimulation of serum IGF-1 level by RA in tumor-bearing rats.

Animals↗

Evaluation of genotoxicity of clofazimine, an antileprosy drug, in mice in vivo. I. Chromosome analysis in bone marrow and spermatocytes.

Clofazimine, an antileprosy drug, was tested for its cytogenetic effect in mouse bone marrow and testis. Bone marrow metaphase analysis in adults treated directly for different periods (1, 2 and 4 weeks, 40 mg/kg/day) and with different doses (4, 20 and 40 mg/kg/day for 7 days) as well as in young animals exposed through lactation for different periods (2, 3, and 4 weeks) revealed significant increases in chromosomal aberrations over the controls. Analysis of diakinesis-metaphase I stages also exhibited a significantly elevated incidence of chromosome aberrations over controls after treatment for different periods. On the basis of the present result the drug may be considered a potential clastogen in mice.

Animals↗

Evaluation of genotoxicity of clofazimine, an antileprosy drug, in mice in vivo. II. Micronucleus test in bone marrow and hepatocytes.

The antileprosy drug, clofazimine, was tested for its possible genotoxicity using micronucleus (MN) tests in mice. A significantly higher incidence of MN in bone marrow erythrocytes, particularly in polychromatic erythrocytes, as well as in regenerated hepatocytes revealed a positive clastogenic effect of the drug. The drug also had a marked antimitotic effect as indicated by a negative correlation with the dose.

Animals↗

Growth-promoting effect of retinoic acid in transplantable pituitary tumor of rat.

MtT/F84 grew well in Fischer rats (F344), but tumor growth was promoted in hyperestrogenized rats. Effects of dietary retinoic acid (RA) on tumor growth, estrogen receptor (ER) and serum growth hormone (GH) level were examined. Tumor latency became shortened, and tumor take and weight were promoted by all-trans RA at dosages of 50 and 200 mg/kg basal diet, but not dose-dependently. ER level was elevated in tumor of RA-treated rats, whereas the retinoic acid-binding protein level remained unchanged. RA also elevated incorporation of 5-bromo-2'-deoxyuridine, a thymidine analogue, into DNA of tumor cells. Average serum GH level was increased in tumor-bearing rats treated with RA and was well correlated with tumor weight. RA may directly affect ER level and enhance estrogenic action, resulting in promotion of tumor growth, or it may act independently for tumor growth and elevation of serum GH level.

Animals↗

Immunologic and survival studies in mice immunised with cryodestroyed ascites fibrosarcoma (AFS) cells.

Adult mice weighing 20 g, divided into 5 groups, were immunised with single and three cycle cryodestroyed ascites fibrosarcoma cells using different modes of immunisation with respect to dose and administration frequency. Survival against subsequent challenge with the same tumour cells and immune response given by leucocyte migration inhibition, were studied in these animals. It appears from the present study that animals with relatively low antigenic load have a significantly (P less than 0.001) high mean survival time and/or survival index compared with controls than those with relatively high antigenic load. Survival in animals immunised with three cycle cryodestroyed tumour cells is significantly (P less than 0.001) higher than that with single cycle even in face of a greater challenging tumour dose with mode of immunisation remaining the same. Preliminary observations on percentage leucocyte migration inhibition showed that there exists no difference in percentage inhibition with either viable or cryodestroyed tumour cell antigens added to migration chamber excepting in animals immunized with three cycle cryodestroyed tumour cells where percentage inhibition was significantly (P less than 0.001) greater with viable than with cryodestroyed tumour cell antigen without altering the percentage inhibition between the two groups. Factors maximising immune response and their modulating effects are discussed.

Animals↗

Application of the least-squares method in the matrix form: simultaneous spectrophotometric determination of rifampicin and isoniazid in binary pharmaceutical formulations.

A least-squares method in the matrix form is described for the simultaneous determination of rifampicin and isoniazid in a mixture. The method allows the rapid analysis of binary pharmaceutical formulations with minimum error. The concentration of each component in the mixture has been determined spectrophotometrically by measuring the absorbance of the mixture at 5-nm intervals from 230 to 290 nm. To calculate the matrix of the proportionality constant a standard mixture was used for each component. All data analyses were performed on a personal computer.

Isoniazid↗

Induction of cataract in methylnitrosourea treated Fischer (F344) rats.

Cataracts were observed in female F344 rats who intravenously received methylnitrosourea (MNU), a potent carcinogen for multiple organs, in one dose of 50 mg/kg body weight. Induction of cataract 40 weeks after MNU treatment was 41% whereas no cataract was observed in control rats. The aggregation of beta H-crystallin fraction by MNU was studied in vitro. HPLC pattern of beta H-crystallin changed when lens protein was incubated for 24 hrs with MNU. HPLC patterns indicated that MNU induced high molecular weight aggregates of beta H-crystallin. This study conveys some indication about the direct interaction of MNU with lens protein in cataract formation.

Animals↗

Increase of estrogen receptor level by thyroxine in estrogen dependent pituitary tumor (MtT/F84) in rats.

A rat transplantable pituitary tumor, MtT/F84, grows much faster in E2 treated rats than in normal females, but is much retarded in thyroidectomized rats. Triiodothyronine (T3) administration in a drinking water increased the tumor growth by the dose dependent manner. The tumor contained both estrogen receptor (ER) and T3 receptor. ER levels both in the nuclei and cytosols elevated 2 to 3 times by the T3 administration compared to those of control. E2 administration promotes the growth of MtT/F84 through elevation of nuclear ER level. T3 may directly elevate cellular ER level and thus it may enhance estrogenic actions including the tumor growth.

Animals↗

Genotoxic potential of diethylcarbamazine, an antifilarial drug.

The mutagenic potential of diethylcarbamazine (DEC) was evaluated by metaphase chromosome analysis and the micronucleus test in bone marrow cells of mice. Both assay systems revealed clastogenicity, although not severe. The time-response study of metaphase chromosomes exhibited an early effect; at 72 h post-treatment the effect came down to the control level. The dose-response analysis showed significantly elevated frequencies of micronuclei (MN) for all the doses tested, but failed to show any influence of the dose on the induction of MN. Data on mitotic index (MI) indicated a lack of cell cycle inhibition. The decrease in aberration frequency with the lapse of time can be probably attributed to the elimination of the drug and its metabolites from the body.

Administration, Oral↗

A three-dimensional digitization method to measure trunk muscle lines of action.

A method is presented to obtain lines of action of nine major trunk muscles acting on the lumbar spine. The muscles are modeled as one or several linear or curvilinear lines of action obtained by digitizing the origins and insertions of main bundles of muscles. For curvilinear lines of action, points were also digitized along the length of the bundles, and a curve-fitting method was used. Lines of action are displayed graphically for one embalmed cadaver. Tabulated data for unit force vectors and unit moment vectors about the L3-L4 and L4-L5 discs are also presented.

Adult↗

A simplified method for micronucleus preparation from hepatic cells.

A simplified method for micronucleus preparation from regenerating hepatocytes has been developed. Small pieces of the regenerating portion of the liver are incubated in 1% sodium citrate solution containing collagenase Type 1A (final concentration 0.005% w/v) at 37 C for 10-15 min with occasional gentle agitation. The larger particles are discarded. Drops of the thick homogeneous citrate suspension of liver cells are put on the slides and drawn back immediately into the pipette, leaving only the drop marks. This simplified method, which gives good preparations with many intact hepatocytes, was validated in a model experiment using mitomycin C. The data revealed a distinct dose-response effect.

Animals↗

Cytogenetic effect of dapsone, an antileprotic drug, in the mouse in vivo system.

Potential genotoxicity of dapsone was evaluated in mice following in vivo cytogenetic assays. Adult male mice treated with different doses (20 mg, 40 mg, or 80 mg/kg/day for 4 weeks) and for different periods (40 mg/kg/day for 2, 4, or 8 weeks) provided bone marrow and testes for mitotic and meiotic chromosome analyses, respectively. A dose-response (20 mg, 40 mg, or 80 mg/kg/day for 2 weeks) analysis was done with a separate set of mice using a micronucleus test (MNT). Untreated mice served as controls. Both the metaphase analysis and MNT in bone-marrow cells revealed significantly higher incidences of clastogenicity for all of the dose levels and treatment periods. Chromosome aberrations, with and without gaps, in bone-marrow metaphases showed a positive correlation with the doses, but not with the treatment periods. Correlation was also lacking in the MNT. In the meiotic cells, the incidences of chromosome aberrations increased significantly with the highest dose and with the longest period of treatment.

Animals↗

[Influence of bandages on the strength of impact of punches in boxing].

In boxing, a punch can be directed to the head or the torso of the opponent; however, because of the gravity of the ensuing injuries, the limit of tolerance of the head and face to repeated impacts (Gurjian, 1955, 1962; Patrick, 1955; Hodgson and Nakamura, 1968) is particularly important to keep in mind. The objective of this research was to compare the impact forces which could be attained barehand versus those obtained with different types of bandages made up of gauze and diachylon. For the purpose of this study, 22 boxers were selected. The impact force was measured with a Kistler force plate, covered with a synthetic mattress. A system of photoelectric cells was used to measure the velocity of the punch. Each boxer had to impact the target with 10 punches while his hand was covered with one of four types of bandages as well as barehand. This study has shown that the bandaged hand increases significantly the impact force of the punch. The force increases in relation to the thickness of the bandage. The use of additional pieces of diachylon has a greater influence than gauzes. Therefore, it can be concluded that the bandaging increases the rigidity of the hand and facilitates the transfer of the force in comparison to a similar punch without a bandage or with a thinner one.

Adolescent↗