Biomedical subjects

B Rosenbusch

Publications and source records attributed to B Rosenbusch.

59 records · Page 4Linked to original sources

PubMed1988

[In vitro fertilization: a comparison with results of the hamster ova penetration test (HOPT)].

The hamster ova penetration test (HOPT) was performed in 82 patients simultaneously with in vitro fertilization of homologous oocytes (IVF). A penetration rate of greater than 20% of inseminated ova was considered to represent a positive test result. The results of HOPT correlated completely with the IVF in all couples with male factor sterility. In couples with female sterility only and in couples with a combination of female and male factors HOPT was falsely negative in 22 and 20% respectively. Despite a negative HOPT in vitro fertilization with formation of two pronuclei 12 to 18 hours after insemination occurred in these patients. Obviously, the interpretation of HOPT is dependent upon the definition of the cut-off point (in our case 20%). Our results support the opinion that there should be no exclusion from an IVF programme based only on a negative HOPT. It appears that any penetration rate greater than 0% should be considered as a sign of potential fertility.

Animals↗

PubMed1988

[Experiences and successes with intravaginal fertilization and culture of human oocytes].

We present in this study our first experience with intravaginal culture (IVC) of human oocytes as a modified technique for in-vitro fertilization. Up to 4 oocytes and washed, pre-incubated spermatozoa (final concentration: 0.1-0.2 x 10(5)/ml) are pipetted into a plastic capsule. The capsule is closed, avoiding any air inclusion. It is placed into the maternal vagina for up to 50 hours, held in place by a diaphragm. The first 15 patients treated by IVC achieved a fertilization rate of 58% (35/60). Following transfer of the cleavage stages, 3 intact clinical pregnancies were established: 2 in the group with tubal sterility factors and 1 in the group of patients with endometriosis. In the group with andrological causes of sterility, one beta-hCG-positive reaction was detected. However, no intact clinical pregnancy was achieved. Our preliminary results show IVC to be a promising alternative in particular cases of sterility treatment. In contrast to the conventional technique, IVC minimizes exposure of gametes to factors which may affect their early development, such as light or low temperature. Furthermore, there is an important psychological factor to be considered, as the patient actively participates in the incubation period of in-vitro fertilization.

Embryo Transfer↗

PubMed1988

[Detection of Chlamydia trachomatis in routine clinical practice: increased sensitivity of cell culture in comparison with the microimmunofluorescence test].

Recently, direct immunofluorescence staining has been reported as a new tool for detection of Chlamydia trachomatis. This assay is based on monoclonal antibodies directed against a protein present in all serological variations of Chlamydia known to date. In order to evaluate whether immunofluorescence staining could substitute for the time-consuming, expensive tissue culture technique, urethral and cervical swabs of 131 patients treated for sterility were examined for infection with Chlamydia by both methods. The tissue culture technique, based on cultivation of Chlamydia in mammalian cells, was regarded as 100% sensitive and specific. Employing this method, we demonstrated infection with Chlamydia in 20 patients (15.3%). Colonization of both, cervix and urethra was found in 11.5%, whereas solitary colonization of cervix and urethra was found in 2.3 and 1.5% respectively. However, the immunofluorescence assay applied ("Micro-Trak", Syva Company) showed positive results in only 6 out of 20 cases as determined by tissue culture, thus yielding a sensitivity of 30%. Out of 111 patients with negative results in the tissue culture, 108 were detected by immunofluorescence staining, amounting to a specificity of 97.3%. With special regard to the low sensitivity of the direct immunofluorescence assay, our results indicate a superior, yet unequalled role of the tissue culture technique for detection of Chlamydia trachomatis.

Bacteriological Techniques↗

PubMed1988

Cytogenetics of unfertilized human oocytes.

During an in-vitro fertilization programme 150 oocytes from 62 women with a mean age of 31 years (range 24-39) remained unfertilized. Successful chromosome analysis was carried out on 96 oocytes by Q-banding: 59 (61.5%) oocytes bore a normal haploid complement, 8 (8.3%) were diploid and 3 (3.1%) tetraploid. In 26 (27.1%) oocytes aneuploidy was observed; these included 9 (9.4%) nullisomic, 5 (5.2%) double nullisomic, 4 (4.2%) triple nullisomic and 2 (2.1%) disomic oocytes. The remaining 54 (36.0%) oocytes could not be evaluated. A nearly uniform rate of aneuploidy was found for unfertilized oocytes among different donor age groups.

Adult↗

PubMedDate not supplied

[Analysis of the serum concentration of pregnancy protein-1 (SP-1) offers no advantage in comparison with menstrual anamnesis and ultrasound biometry in determining gestational age].

In 69 patients with normal pregnancy the estimated day of confinement (EDC) was determined by three different methods (last menstrual period = LMP, ultrasound, and determination of Schwangerschaftsprotein-1 (SP-1) serum concentration). Ultrasound and LMP were shown to predict the EDC with approximately the same accuracy. While data obtained by LMP were scattered a little bit more around the mean value, data obtained by ultrasound tended to underestimate the EDC slightly. Determination of SP-1 serum concentration had the lowest predictive value in assessing the EDC. Accordingly, we don't consider routine SP-1 determinations to be a useful test in antenatal care.

Female↗