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Biomedical subjects

B Roe

Publications and source records attributed to B Roe.

At least 73 records · Page 4Linked to original sources
PubMed1995

Cloning a balanced translocation associated with DiGeorge syndrome and identification of a disrupted candidate gene.

DiGeorge syndrome (DGS), a developmental defect, is characterized by cardiac defects and aplasia or hypoplasia of the thymus and parathyroid glands. DGS has been associated with visible chromosomal abnormalities and microdeletions of 22q11, but only one balanced translocation--ADU/VDU t(2;22)(q14;q11.21). We now report the cloning of this translocation, the identification of a gene disrupted by the rearrangement and the analysis of other transcripts in its vicinity. Transcripts were identified by direct screening of cDNA libraries, exon amplification, cDNA selection and genomic sequence analysis using GRAIL. Disruption of a gene in 22q11.2 by the breakpoint and haploinsufficiency of this locus in deleted DGS patients make it a strong candidate for the major features associated with this disorder.

Amino Acid Sequence↗
PubMed1995

A longitudinal primary care program in an urban public medical school: three years of experience.

The experience of the University of Illinois at Chicago's College of Medicine with implementing a pilot generalist program focuses on institutionalization and management. Various features of the program make it an interesting case study: It is inter-disciplinary, comprising pediatricians, general internists, and family practitioners; students join the program in the autumn of their first year; and it is changing from a voluntary to a required, institutionally ingrained course of study. The difficulties and procedures encountered in making room for an interdisciplinary primary care program in a traditional medical school curriculum are discussed.

Chicago↗
PubMed1995

Communication as an essential part of program and institutional development.

The Longitudinal Primary Care Program, an innovative curriculum in generalist medicine at the University of Illinois at Chicago College of Medicine, has been enhanced by a commitment to fostering good communication among all of its participants (who include more than 350 students and nearly 300 office-based generalist preceptors) and between participants and administrators. The diverse and widely scattered group of preceptors is brought together through orientation sessions and faculty development workshops that stress precepting skills and mentoring. Preceptors are regularly surveyed and the survey data are analyzed to help administrators learn how best to recruit and retain these faculty members. Students' input comes through surveys, small-group meetings, and their participation on committees. Program evaluation data and formal notes from committee meetings are promptly compiled and disseminated. Such efforts to promote good communication among preceptors, students, program administrators, and the medical college have both enhanced the generalist program and encouraged other curricular reforms in the college.

Chicago↗
PubMed1992

Reducing nosocomial urinary tract infections in geriatric patients.

1. The urinary tract is the most common site of hospital-acquired infections, accounting for as many as 42% of all such infections. 2. Infection control is a team effort from the emergency room, to the operating room, to each separate nursing unit. A break in technique anywhere along the line poses increased risk. 3. Infection control must be a continuing effort of education, awareness, getting back to basics, and improved infection control techniques.

Aged↗
PubMed1990

Nursing practice for catheter care.

Catheter care forms a frequent part of nursing practice, with the ever important need for nurses to be aware of current research evidence and developments. This paper critically reviews the literature and research evidence for catheter care and presents recommendations for nursing practice.

Catheters, Indwelling↗
PubMed1985

Nucleotide sequence of the streptokinase gene from Streptococcus equisimilis H46A.

The entire nucleotide sequence of a cloned 2568-bp PstI fragment from the genome of Streptococcus equisimilis H46A encoding the streptokinase gene (skc) has been determined. The longest open reading frame comprises 1320 bp which code for streptokinase. The protein is synthesized with a 26-amino acid residue N-terminal extension having properties characteristic of a signal peptide. Comparison of the deduced amino acid sequence with the available amino acid sequence of a commercial streptokinase reveals minor primary structure differences. The nucleotide sequencing of skc does not support the hypothesis that the gene has evolved by duplication and fusion, as suggested by internal twofold amino acid homologies of its product. Furthermore, the skc gene sequence shows no extended regions homologous to the staphylokinase gene. Upstream from the skc gene, the putative skc promoter and the ribosome-binding site sequence have been identified; downstream from the coding region, inverted repeat sequences thought to function as transcription terminators have been detected.

Amino Acid Sequence↗