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Biomedical subjects

B Richter

Publications and source records attributed to B Richter.

At least 181 records · Page 10Linked to original sources
PubMed1977

[Determination of heat stable alkaline phosphatase].

In this work the method for determination of serum heat-stable alkaline phosphatase (HSAP)--valuable as a placenta function test in pregnancy--is discussed. There are different informations about the optimal conditions for inactivating the nonplacental heat labile isoenzyms, and therefore the opinions on the clinical efficiency of HSAP vary in different studies. Sera of nonpregnant and pregnant females were heated at 40 degrees to 65 degrees C for 5 to 60 minutes. It is demonstrated, that in opposite to suggested opinions in several reports a temperature of 56 degrees C for 30 minutes is notsufficient to inactivate all nonplacental alkaline phosphatase isoenzymes. At 60 degrees C for 20 minutes and at 65 degrees C for 5 minutes in sera of pregnant and nonpregnant female controls we could find concordant results, so that these two conditions of inactivation are regarded as the best. We prefer a temperature of 60 degrees C and inactivate for 20 minutes, since these conditions are to reproduce more easy and allow a beter precision of the results, than 65 degrees C for 5 minutes.

Alkaline Phosphatase↗
PubMed1977

[Biochemical investigations for interpretation of in vivo measurements of 32P-incorporation into irradiated or nonirradiated experimental tumors (author's transl)].

In view of results of extracorporal measurements of the 32 P-incorporation into irradiated or nonirradiated tumors biochemical investigations of the 32P-incorporation have been performed, aiming at comprehension of the meaning and at clarification of the interpretability of these measurements. The results show that irradiation mostly decreases the content of 32P in nucleic acids. In principle, however, the latter is so low as compared to the total content of 32P, especially three hours after 32P-injection, that variations are hardly to be recorded by means of extracorporal measurements, particularly because they are superposed by changes in the 32P-content of other fractions.

Animals↗